Midgut Bacterial Microbiota of 12 Fish Species from a Marine Protected Area in the Aegean Sea (Greece)

Kormas, Konstantinos Ar.; Nikouli, Eleni; Kousteni, Vasiliki; Damalas, Dimitrios

doi:10.1007/s00248-022-02154-x

Cited by 6 publications

References 73 publications

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The intestinal digesta microbiota of tropical marine fish is largely uncultured and distinct from surrounding water microbiota

Soh,

Tay,

Lee

et al. 2024

npj Biofilms Microbiomes

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Studying the gut microbes of marine fishes is an important part of conservation as many fish species are increasingly threatened by extinction. The gut microbiota of only a small fraction of the more than 32,000 known fish species has been investigated. In this study we analysed the intestinal digesta microbiota composition of more than 50 different wild fish species from tropical waters. Our results show that the fish harbour intestinal digesta microbiota that are distinct from that of the surrounding water and that location, domestication status, and host intrinsic factors are strongly associated with the microbiota composition. Furthermore, we show that the vast majority (~97%) of the fish-associated microorganisms do not have any cultured representative. Considering the impact of the microbiota on host health and physiology, these findings underpin the call to also preserve the microbiota of host species, especially those that may be exposed to habitat destruction.

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The intestinal digesta microbiota of tropical marine fish is largely uncultured and distinct from surrounding water microbiota

Soh,

Tay,

Lee

et al. 2024

npj Biofilms Microbiomes

View full text Add to dashboard Cite

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The effect of dietary fish oil replacement by microalgae on the gilthead sea bream midgut bacterial microbiota

Katsoulis-Dimitriou,

Nikouli,

Gkalogianni

et al. 2024

Preprint

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The aim of this work was to evaluate the effects of dietary microalgae blends as fish oil replacers οn the midgut bacterial microbiota of gilthead sea bream (Sparus aurata). The control diet (FO) contained only fish oil as source of lipids, EPA and DHA fatty acids, while three experimental diets were used where fish oil was replaced at 67% by one of the following microalgae biomass blends: Michrochloropsis gaditana and Isochrysis sp. (MI), Phaeodactylum tricornutum and Isochrysis sp. (PI) and Schizochytrium sp. and P. tricornutum (SP). The midgut bacterial community composition and the dominant OTUs indicated that the sea bream midgut bacterial communities were altered compared to the control feed. Additional evidence from the presumptive bacterial functional pathways suggests that the microalgal-containing aquafeed resulted in one overexpressed and one underexpressed pathway. The overexpressed pathway was related to the metabolism of fucose, a major carbohydrate of these microalgae species. This suggests that a new gut microbiota profile was selected because of the microalgae inclusion in the provided aquafeed. All these data combined with the absence of mortality in fish, shows that the gilthead sea bream gut microbiome can smoothly adapt its function according to the metabolic capacity of the microalgae combinations that we used. The MI feed seems to promote several beneficial bacteria with potential probiotic abilities in the fish gut.

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Geographic origin and host’s phylogeny are predictors of the gut mucosal microbiota diversity and composition in Mediterranean scorpionfishes (Scorpaena sp.)

Lilli,

Sirot,

Campbell

et al. 2023

Preprint

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Background The gut microbiome holds an important role in the health and homeostasis of fishes. However, despite the large diversity and distribution of this vertebrate group, only the intestinal microbiome of a limited number of freshwater and marine fish species has been well characterized to date. Mediterranean scorpionfishes (Scorpaena sp) have a high commercial value in the Mediterranean Sea and Scorpaena scrofa is a candidate to become an aquaculture-reared species in a near future. However, this fish group is underrepresented in studies about fish gut microbiome. Results In this study, we characterized the gut mucosal microbial communities of three Scorpaena sp (n = 125) by using a comprehensive comparative dataset including 16S rRNA gene amplicon data from four different locations in the Mediterranean Sea. We report that in this fish group, geographical origin influences the diversity and the composition of the gut microbial communities more than host’s phylogenetic relatedness. Moreover, we observe a positive correlation between the composition of the gut microbiota and the phylogenetic distance between the hosts (i.e. phylosymbiosis). Finally, the core microbiota of each species was described both regionally and across the Mediterranean Sea. When investigated regionally, the core microbiota was characterized by bacterial genera holding important functions for the health and homeostasis of the host. By expanding the analysis to a broader geographical scale, a reduction in the size of the core microbiota of each species was observed and Clostridium senso stricto 1 appeared to be the only resident bacterial genus in the core gut microbiota of Scorpaena sp in the Mediterranean Sea. Conclusion This study reveals that the microbiota associated with the gut mucosa of scorpionfishes is not stable across the Mediterranean Sea and its composition depends on the fish population analyzed. Therefore, it highlights the importance of investigating the gut microbiome through the geographical range of a species and it suggests this as a general procedure to better characterize the gut microbial ecology of each fish species

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Midgut Bacterial Microbiota of 12 Fish Species from a Marine Protected Area in the Aegean Sea (Greece)

Cited by 6 publications

References 73 publications

The intestinal digesta microbiota of tropical marine fish is largely uncultured and distinct from surrounding water microbiota

The intestinal digesta microbiota of tropical marine fish is largely uncultured and distinct from surrounding water microbiota

The effect of dietary fish oil replacement by microalgae on the gilthead sea bream midgut bacterial microbiota

Geographic origin and host’s phylogeny are predictors of the gut mucosal microbiota diversity and composition in Mediterranean scorpionfishes (Scorpaena sp.)

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