Isolation of CD4&lt;sup&gt;+&lt;/sup&gt; T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry

Byford, Elliot; Carr, Matthew; Pinòn, Lucia; Ahearne, Matthew J.; Wagner, Simon D.

doi:10.3791/58431

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2021

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“…Although T FH cells are predominantly located in the secondary lymphoid organs, circulating T FH cells can be identified in the blood. 13,14 Circulating T FH 13 cells can either be identified using nonspecific activation with PMA/ionomycin or specific activation with allergen. 9,15 While the latter has the advantage of resolving numbers of antigen-specific T FH 13 cells, this is only possible when the antigen is known.…”

Section: Introductionmentioning

confidence: 99%