Isolation of carboxylesterase (esterase FE4) from  Apis cerana cerana  and its role in oxidative resistance during adverse environmental stress

Ma, Manli; Jia, Hongyu; Cui, Xuepei; Zhai, Na; Wang, Hongfang; Guo, Xingqi; Xu, Baohua

doi:10.1016/j.biochi.2017.10.022

Cited by 29 publications

(26 citation statements)

References 59 publications

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“…In insects, esterase proteins are involved in many BPs, such as detoxification of environmental toxins (e.g., plant materials), development, courtship behaviour and reproduction (Bloch, Heifetz, & Wolfner, 2003;Mackert, do Nascimento, Bitondi, Hartfelder, & Simões, 2008;Mane, Tompkins, & Richmond, 1983). Interestingly, it has been shown that low temperature (4ºC) affects esterase enzyme activity in A. cerana (Ma et al, 2018). In D. melanogaster, the reproductive fitness of males is strongly affected by temperature and the esterase-6 alleles TA B L E 2 (Continued) they carry.…”

Section: Identification Of Outlier Snps and Candidate Regions Of Sementioning

confidence: 99%

The genomic basis of adaptation to high‐altitude habitats in the eastern honey bee (Apis cerana)

et al. 2019

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The eastern honey bee (Apis cerana) is of central importance for agriculture in Asia. It has adapted to a wide variety of environmental conditions across its native range in southern and eastern Asia, which includes high‐altitude regions. eastern honey bees inhabiting mountains differ morphologically from neighbouring lowland populations and may also exhibit differences in physiology and behaviour. We compared the genomes of 60 eastern honey bees collected from high and low altitudes in Yunnan and Gansu provinces, China, to infer their evolutionary history and to identify candidate genes that may underlie adaptation to high altitude. Using a combination of FST‐based statistics, long‐range haplotype tests and population branch statistics, we identified several regions of the genome that appear to have been under positive selection. These candidate regions were strongly enriched for coding sequences and had high haplotype homozygosity and increased divergence specifically in highland bee populations, suggesting they have been subjected to recent selection in high‐altitude habitats. Candidate loci in these genomic regions included genes related to reproduction and feeding behaviour in honey bees. Functional investigation of these candidate loci is necessary to fully understand the mechanisms of adaptation to high‐altitude habitats in the eastern honey bee.

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Section: Identification Of Outlier Snps and Candidate Regions Of Sementioning

confidence: 99%

The genomic basis of adaptation to high‐altitude habitats in the eastern honey bee (Apis cerana)

et al. 2019

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“…In addition, the ROS generated gets scavenged by the low-molecular-weight free-radical scavengers and defensive enzymes including PPO, POD, CAT, and SOD (Qin, Lu, Zheng, & Du, 2016). These enzymes are the determiners of oxidative resistance under adverse environmental stress conditions (Chen et al, 2014;Ma et al, 2017;Paithankar, Raghu, & Patil, 2018). Thus, a study to understand the role of entomic antioxidants in resistance to pesticides could be helpful.…”

Section: Discussionmentioning

confidence: 99%

Variation in the pH of experimental diets affects the performance of Lymantria dispar asiatica larvae and its gut microbiota

Zeng

Shi

Guo

et al. 2020

Arch Insect Biochem Physiol

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To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5.00 in DPB6, 6.05 in control, and 6.50 in DPB8. After feeding on the diets with different pH values for 84 hr, fourth‐instar caterpillars were randomly collected. Growth and various physiological traits were determined and 16S recombinant DNA sequencing was performed using the intestinal microflora of surviving larvae. Results showed that the mortality was 30% in DPB6, and 10% in DPB8, while no mortality was observed in control. The partial least squares discriminant analyses suggested that diets prepared with PB of different pH resulted in different food intake, amount of produced feces, weight gain, digestive enzyme activities, and antioxidant enzyme activities in larvae. Interestingly, both the highest weight gain and the lowest total antioxidant capacities were seen in control larvae. Results also showed that the larval gut microbiota community structure was significantly affected by dietary pH. Moreover, linear discriminant analysis effect size suggested that the family Acetobacteraceae in control, genus Prevotella in DPB8, and genus Lactococcus, family Flavobacteriaceae, family Mitochondria, and family Burkholderiaceae in DPB6 contributed to the diversity of the larval gut microbial community.

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“…Thus, it appears that carboxylesterases are modulated by gamma rays in the honeybee, but in a way difficult to understand from a biological point of view. Conversely, in Apis cerana cerana, a more obvious response can be observed, with an increase in the expression of carboxylesterase after UV radiation (Ma et al, 2018). Carboxylesterases seem to be particularly sensitive to pollutants or radiation in insects, molluscs and rodents with responses that are very specific because they are not associated to a modulation of tissue protein content (Auda et al, 1987;Badiou-Bénéteau et al, 2012a;Carvalho et al, 2013a;Fleming et al, 2016;Franco et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Physiological effects of gamma irradiation in the honeybee, Apis mellifera

Gagnaire

Bonnet²,

Tchamitchian³

et al. 2019

Ecotoxicology and Environmental Safety

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Terrestrial ecosystems are exposed to various kinds of pollutants, including radionuclides. The honeybee, Apis mellifera, is commonly used in ecotoxicology as a model species for evaluating the effects of pollutants. In the present study, honeybees were irradiated right after birth for 14 days with gamma rays at dose rates ranging between 4.38x10-3 and 588 mGy/d. Biological tissues (head, intestine and abdomen) were sampled at D3, D10 and D14. Ten different physiological markers involved in nervous (acetylcholinesterase (AChE)), antioxidative (catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione-S-transferase (GST)), immune system (phenoloxidase (PO)) and metabolism (carboxylesterases (CaEs) and alkaline phosphatase (ALP)) were measured. Univariate analyses were conducted to determine whether each individual biomarker response was positively or negatively correlated with the dose rate. Then, multivariate analyses were applied to investigate the relationships between all the biomarker responses. Although no mortality occurred during the experiment, several biomarkers varied significantly in relation to the dose rate. Globally, the biomarkers of antioxidant and immune systems decreased as the dose rate increased. Reversible effects on the indicator of the neural system were found. Concerning indicators of metabolism (carboxylesterases), variations occurred but no clear pattern was found. Taken altogether, these results help better understand the effects of ionizing radiation on bees by identifying relevant physiological markers of effects. These results could improve the assessment of the environmental risk due to ionizing radiation in terrestrial ecosystems.

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Isolation of carboxylesterase (esterase FE4) from Apis cerana cerana and its role in oxidative resistance during adverse environmental stress

Cited by 29 publications

References 59 publications

The genomic basis of adaptation to high‐altitude habitats in the eastern honey bee (Apis cerana)

The genomic basis of adaptation to high‐altitude habitats in the eastern honey bee (Apis cerana)

Variation in the pH of experimental diets affects the performance of Lymantria dispar asiatica larvae and its gut microbiota

Physiological effects of gamma irradiation in the honeybee, Apis mellifera

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