Isolation of Carbazole Alkaloids and Coumarins from Aegle marmelos and Murraya koenigii and Their Antioxidant Properties

Rou, Chian Ng; Kassim, Nur Kartinee; Soon, Yunie Yu Yeap; Cheng, Gwendoline Lian Ee; Yazan, Saiful Latifah; Musa, Khalid Hamid

doi:10.17576/jsm-2018-4708-14

Cited by 20 publications

(10 citation statements)

References 15 publications

(20 reference statements)

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“…Thus, spectrophotometric variation of the solution's color (a decrease in absorbance at approximately 470 nm) is representative of the generation of lipid peroxides. The procedure, advantages, and disadvantages of this system have been extensively discussed in the literature [130][131][132][133][134][135][136][137][138], and some examples of the latest LPIP research employing this method are suggested [139][140][141][142][143][144][145][146][147][148][149].…”

Section: Watermentioning

confidence: 99%

Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage

et al. 2020

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Lipid peroxidation is a chemical reaction known to have negative impacts on living organisms’ health and on consumer products’ quality and safety. Therefore, it has been the subject of extensive scientific research concerning the possibilities to reduce it, both in vivo and in nonliving organic matrices. It can be started by a variety of oxidants, by both ROS-dependent and -independent pathways, all of them reviewed in this document. Another feature of this reaction is the capacity of lipid peroxyl radicals to react with the non-oxidized lipids, propagating the reaction even in the absence of an external trigger. Due to these specificities of lipid peroxidation, regular antioxidant strategies—although being helpful in controlling oxidative triggers—are not tailored to tackle this challenge. Thus, more suited antioxidant compounds or technologies are required and sought after by researchers, either in the fields of medicine and physiology, or in product development and biotechnology. Despite the existence of several laboratory procedures associated with the study of lipid peroxidation, a methodology to perform bioprospecting of natural products to prevent lipid peroxidation (a Lipid Peroxidation Inhibitory Potential assay, LPIP) is not yet well established. In this review, a critical look into the possibility of testing the capacity of natural products to inhibit lipid peroxidation is presented. In vitro systems used to peroxidize a lipid sample are also reviewed on the basis of lipid substrate origin, and, for each of them, procedural insights, oxidation initiation strategies, and lipid peroxidation extent monitoring are discussed.

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Section: Watermentioning

confidence: 99%

Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage

et al. 2020

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“…Naturally occurring compounds with anticancer effects have gained attention due to their low toxicity [ 3 ]. Murrayanine is a carbazole alkaloid derived from Murraya koenigii, which has been used in traditional Chinese medicine in the treatment of cancer [ 4 ] . Carbazole alkaloids have been shown to exhibit anticancer effects against a range of cancers [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Murrayanine Induces Cell Cycle Arrest, Oxidative Stress, and Inhibition of Phosphorylated p38 Expression in A549 Lung Adenocarcinoma Cells

Zhang

Gao

et al. 2019

Med Sci Monit

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BackgroundMurrayanine is a carbazole alkaloid derived from Murraya koenigii, which has been used in traditional Chinese medicine in the treatment of cancer. This study aimed to investigate the effects of murrayanine on human lung adenocarcinoma cells in vitro and to investigate the mechanisms of its action.Material/MethodsA549 human lung adenocarcinoma cells and MRC-5 human lung fibroblasts were grown in culture, and an MTT assay determined cell viability. Cells were treated for 24 h with increasing doses of murrayanine (0, 9, 18, and 36 μM). Fluorescence, using 4′, 6-diamidino-2-phenylindole (DAPI), acridine orange, ethidium bromide, and propidium iodide (PI), were used for the detection of apoptosis. The cell cycle was studied with fluorescence-activated cell sorting (FACS), and Western blot evaluated protein expression.ResultsMurrayanine treatment resulted in significant dose-dependent inhibition of the growth of A549 cells (p<0.05), with an IC50 of 9 μM, and arrested the cells at the G2/M phase of the cell cycle, reduced the expression of cyclin D and E, CDK2, 4, and 6, and increased the expression of p21 and p27. Murrayanine treatment increased apoptosis of the A549 cells and increased cleaved of caspase-3 and caspase-9, and the Bax/Bcl-2 ratio. Murrayanine treatment increased levels of reactive oxygen species (ROS), disrupted the mitochondrial membrane potential, inhibited invasion, and inhibited phosphorylation of p38 mitogen-activated protein kinase (MAPK) of the A549 cells.ConclusionsMurrayanine induced cell cycle arrest, oxidative stress, and inhibited the expression of phosphorylated p38 in A549 adenocarcinoma cells.

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“…2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical‐scavenging activity was measured by adding samples (1 ml) with 2.9 ml of 0.15 mM methanolic DPPH solution, vortexed, and incubated in the dark for 2 hr at room temperature (Ng et al., 2018). Absorbance measurements were recorded at 517 nm in a 96 well microplate using a microplate spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

“…methanolic DPPH solution, vortexed, and incubated in the dark for 2 hr at room temperature (Ng et al, 2018). Absorbance measurements were recorded at 517 nm in a 96 well microplate using a microplate spectrophotometer.…”

Section: Dpph Free Radical Scavenging Activitymentioning

confidence: 99%

Sequential extraction of red button ginger ( Costus woodsonii ): Phytochemical screening and antioxidative activities

Jin

Chang

Khalid

et al. 2020

J. Food Process. Preserv.

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Costus plant is considered as a medicinal plant especially in therapeutic intervention. The current study reports the composition, phytochemical, and antioxidant properties of red button ginger (Costus woodsonii) using sequential extraction. Solvents of different polarities (hexane, ethyl acetate, methanol, and water) were used to sequentially extract the C. woodsonii bract, at increasing polarities. Phytochemical screening showed positive presence of flavonoids (all types of extracts), terpenoids (only in methanolic extract), and tannins (except aqueous extract). Overall, methanolic extracts showed the significantly (p ≤ .05) highest ascorbic acid content (75.76 ± 1.47 mg/100 ml), total phenolic content (249.64 ± 2.83 mg GAE/100 g), DPPH free radical-scavenging activity (78.76 ± 0.97%), ferric reducing antioxidant power (22.55 ± 0.96 mg TEAC/g), superoxide anion scavenging activity (46.60 ± 1.57%) and hydroxyl radical-scavenging activity (50.35 ± 0.96%). In short, sequential extraction provides understanding of polarity specificity of C. woodsonii plant and result indicated high polarity solvent (methanol and water) recovered higher bioactive compounds. Practical applications Sequential extraction procedures have shown a rapid increase in applications, in a wide range of sample types. This study proposed the use of sequential extraction with solvents of different polarities to elute maximum amount of compounds present in Costus woodsonii plant. Many studies indicated that Costus genus plant exhibited high bioactivities such as anticancer, apoptotic effect, anti-inflammatory, antidiabetics, antifungal, and antibacterial properties which revealed the potential values of these plants for nutraceutical application. However, the study on C. woodsonii is very limited. This report would reveal the assessment of sequential extraction performance and its relationship with the properties of C. woodsonii. This would generate considerable interest to the research community to improve the understanding of this plant. Overall, this report is very beneficial to impart a new information to the food industry as well as the pharmaceutical field, which may broaden the application of this plant as a potential functional crop and bioactive ingredient.

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Isolation of Carbazole Alkaloids and Coumarins from Aegle marmelos and Murraya koenigii and Their Antioxidant Properties

Cited by 20 publications

References 15 publications

Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage

Evaluating the In Vitro Potential of Natural Extracts to Protect Lipids from Oxidative Damage

Murrayanine Induces Cell Cycle Arrest, Oxidative Stress, and Inhibition of Phosphorylated p38 Expression in A549 Lung Adenocarcinoma Cells

Sequential extraction of red button ginger ( Costus woodsonii ): Phytochemical screening and antioxidative activities

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