Sequential extraction of red button ginger ( Costus woodsonii ): Phytochemical screening and antioxidative activities

Abstract

Costus plant is considered as a medicinal plant especially in therapeutic intervention. The current study reports the composition, phytochemical, and antioxidant properties of red button ginger (Costus woodsonii) using sequential extraction. Solvents of different polarities (hexane, ethyl acetate, methanol, and water) were used to sequentially extract the C. woodsonii bract, at increasing polarities. Phytochemical screening showed positive presence of flavonoids (all types of extracts), terpenoids (only in methanolic extract), and tannins (except aqueous extract). Overall, methanolic extracts showed the significantly (p ≤ .05) highest ascorbic acid content (75.76 ± 1.47 mg/100 ml), total phenolic content (249.64 ± 2.83 mg GAE/100 g), DPPH free radical-scavenging activity (78.76 ± 0.97%), ferric reducing antioxidant power (22.55 ± 0.96 mg TEAC/g), superoxide anion scavenging activity (46.60 ± 1.57%) and hydroxyl radical-scavenging activity (50.35 ± 0.96%). In short, sequential extraction provides understanding of polarity specificity of C. woodsonii plant and result indicated high polarity solvent (methanol and water) recovered higher bioactive compounds. Practical applications Sequential extraction procedures have shown a rapid increase in applications, in a wide range of sample types. This study proposed the use of sequential extraction with solvents of different polarities to elute maximum amount of compounds present in Costus woodsonii plant. Many studies indicated that Costus genus plant exhibited high bioactivities such as anticancer, apoptotic effect, anti-inflammatory, antidiabetics, antifungal, and antibacterial properties which revealed the potential values of these plants for nutraceutical application. However, the study on C. woodsonii is very limited. This report would reveal the assessment of sequential extraction performance and its relationship with the properties of C. woodsonii. This would generate considerable interest to the research community to improve the understanding of this plant. Overall, this report is very beneficial to impart a new information to the food industry as well as the pharmaceutical field, which may broaden the application of this plant as a potential functional crop and bioactive ingredient.

Assessment of the Phytochemical Analysis and Antimicrobial Potentials of Zingiber zerumbet

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The Antimicrobial Efficacy Against Selective Oral Microbes, Antioxidant Activity and Preliminary Phytochemical Screening of Zingiber officinale

“…The formation of the red-brown precipitate indicated the presence of the terpenoids. 24 For glycosides, a few drops of 2% ferric chloride, 2 mL of 95% glacial acetic acid, and 1 mL H 2 SO 4 were added in 6 test tubes containing 5 mg extract and 5 ml of 6 different solvents, respectively. Glycoside presence was confirmed by forming the brown or violet coloured ring just at the interface of the two liquids.…”

A Comprehensive Review with Future Prospects on the Medicinal Properties and Biological Activities of Curcuma caesia Roxb.

“…Tere were various antioxidant assays tested on C. caesia, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing activity (FRAP), total antioxidant capacity, thiobarbituric acid reactive species (TBARS) assay, and total phenolic content (TPC). Tese diferent assays measure diferent antioxidant mechanisms; for example, the DPPH assay detects the scavenging activity by donating an electron (primary antioxidant), whereas the FRAP assay determines the ability to reduce ferric to ferrous by donating an electron (primary and secondary antioxidant) [63,64]. Secondary metabolite content in plants is claimed to be the reason for the efectiveness of the plants as herbal medicine.…”