Isolation of Campylobacter from human stool samples

Salim, S. M.; Mandal, Jharna; Sc, Parija

doi:10.4103/0255-0857.124294

Cited by 23 publications

(8 citation statements)

References 18 publications

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“…With regard to the different oxygen atmospheres used in the present work, they were tested because the oxygen levels are different in the human body (14% in the pulmonary alveoli, 12% in the arterial blood, 5.3% in the venous blood) ( Erecińska and Silver, 2001 ; Sharp and Bernaudin, 2004 ; Dubois et al, 2016 ). The microaerophilia formed in the candle jar is a widely used method and is described in the literature as effective for reducing oxygen tension for the growth of some microorganisms in vitro ( Mandal et al, 2014 ; Carr et al, 2015 ). Although the two oxygen tension conditions tested provided a good development of biofilms, in quantitative tests, the condition of microaerophilia was superior to the condition of aerobiosis, particularly in biofilms formed using HAM-F12.…”

Section: Discussionmentioning

confidence: 99%

“…At least 90% viability certified, viable cells were counted, and fungal suspensions were prepared in sterile PBS at 5 × 10 6 céls/mL. Two hundred microliters were added to the 96-well plates (Kasvi), which were incubated for 12 h for pre-adhesion period (pre-established time) in two conditions (aerobiosis, at 37°C with shaking at 150 rpm and in microaerophilia atmosphere formed in candle jar ( Mandal et al, 2014 and Carr et al, 2015 ), at 37°C, without shaking). After pre-adhesion, supernatants were removed, and cells were washed three times with sterile PBS to remove non-adherent cells.…”

Section: Methodsmentioning

confidence: 99%

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Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

et al. 2020

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Histoplasma capsulatum is a dimorphic fungus that causes an important systemic mycosis called histoplasmosis. It is an infectious disease with high prevalence and morbidity that affects the general population. Recently, the ability of these fungi to form biofilms, a phenotype that can induce resistance and enhance virulence, has been described. Despite some efforts, data regarding the impact of nutrients and culture media that affect the H. capsulatum biofilm development in vitro are not yet available. This work aimed to study H. capsulatum biofilms, by checking the influence of different culture media and oxygen atmospheres in the development of these communities. The biofilm formation by two strains (EH-315 and G186A) was characterized under different culture media: [Brain and Heart Infusion (BHI), Roswell Park Memorial Institute (RPMI) with 2% glucose, Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum and nutrient medium HAM-F12 (HAM-F12) supplemented with glucose (18.2 g/L), glutamic acid (1 g/L), HEPES (6 g/L) and L-cysteine (8.4 mg/L)] and oxygen atmospheres (aerobiosis and microaerophilia), using the XTT reduction assay to quantify metabolic activities, crystal violet staining for biomass, safranin staining for the quantification of polysaccharide material and scanning electron microscopy (SEM) for the observation of topographies. Results indicated that although all culture mediums have stimulated the maturation of the communities, HAM-F12 provided the best development of biomass and polysaccharide material when compared to others. Regarding the oxygen atmospheres, both stimulated an excellent development of the communities, however in low oxygen conditions an exuberant amount of extracellular matrix was observed when compared to biofilms formed in aerobiosis, mainly in the HAM-F12 media. SEM images showed yeasts embedded by an extracellular matrix in several points, corroborating the colorimetric assays. However, biofilms formed in BHI, RPMI, and DMEM significantly induced yeast to hyphae reversal, requiring further investigation. The results obtained so far contribute to in vitro study of biofilms formed by these fungi and show that nutrition promoted by different media modifies the development of these communities. These data represent advances in the field of biofilms and contribute to future studies that can prove the role of these communities in the fungi-host interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

et al. 2020

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“…Common long-term complications of campylobacteriosis are Guillain-Barre syndrome, inflammatory bowel disease, bacteremia, rheumatoid arthritis, along with local complications such as cholecystitis, pancreatitis, peritonitis, massive gastrointestinal hemorrhage, thyroiditis, and prosthetic joint infection. Over 2.4 million persons annually or 0.8% of the total population [6] are affected making it a very important organism from a socioeconomic perspective. However, many of the cases go undiagnosed.…”

Section: Introductionmentioning

confidence: 99%

Virulence typing and antibiotic susceptibility profiling of thermophilic Campylobacters isolated from poultry, animal, and human species

et al. 2018

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Background and Aim:Campylobacteriosis finds its place among the four important global foodborne illnesses. The disease, though self-limiting, needs antibacterial therapy in extraintestinal complications. Therefore, the present study was designed to estimate the prevalence of thermophilic Campylobacters in poultry, animals, and humans of the Kumaon region of Uttarakhand.Materials and Methods:A total of 609 samples comprising of poultry ceca (n=116), poultry droppings (n=203), and feces of pigs (n=71), cattle (n=61), sheep (n=19), goat (n=17), human beings (n=88), and laboratory animals (n=34) (rats, rabbits, and guinea pigs) were collected. The thermophilic Campylobacters, Campylobacter jejuni and Campylobacter coli were confirmed using multiplex polymerase chain reaction. The isolates were also screened for the presence of virulence genes, and their antibiotic susceptibility testing was done against eight antibiotics.Results:An overall prevalence of 6.24% was revealed with highest from poultry ceca (15.52%), followed by poultry droppings (5.91%), cattle feces (4.92%), human stools (3.40%), and pig feces (2.82%). The virulence genes, namely cadF, flaA, virB11, and pldA, were present in 38 (100%), 37 (97.37%), 7 (18.42%), and 14 (36.84%) isolates, respectively. All the isolates were resistant to nalidixic acid, while all were sensitive to erythromycin and co-trimoxazole.Conclusion:It was concluded that the animals and humans in the region harbored the thermophilic Campylobacters which may contribute to the human illness. Resistance shown among the isolates may complicate the antimicrobial therapy.

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“…These include oral drugs like TMP-SMX, nitrofurantoin, ciprofloxacin, oral cephalosporins as well as injectables like cephalosporins, aminoglycosides and carbapenems. Hospital acquired strains are known to be more resistant than community acquired ones [5,6].…”

Section: Introductionmentioning

confidence: 99%

Increasing antibiotic resistance amongst Uropathogenic E. coli isolated from inpatients with symptomatic UTI - a three year study

Verma¹,

Shah²,

Hemachander³

2017

Trop J Pathol Microbiol

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Introduction: Escherichia coli (E.coli) is one of the most common uropathogen worldwide. In the past few years, uropathogenic E.coli has become increasingly resistant to many drugs. Materials and Methods: This prospective observational study was conducted from January 2014 to December 2016. Semiquantitative urine culture was done on blood agar and MacConkey agar using standard technique. Cultures showing a significant growth of 10 5 CFU/ml or more were further processed. Identification and analysis of antibiotic susceptibility patterns was done by VITEK ® 2 (bioMerieux, USA). Results: A total of 674 E. coli samples were included in the final analysis. The isolates showed highest overall sensitivity to imipenem (75.82%) and nitrofurantoin (72.25%) whereas highest resistance was observed in case of amoxicillin-clavulinic acid (84.12%) and cephalosporins (71.96-83.23%), followed by ciprofloxacin (72.70%), amikacin (64.39%) and TMP-SMX (64.24%). Over three years duration, a trend of increasing resistance was observed in the case of cephalosporins, ciprofloxacin and trimethoprim-sulfamethoxazole (TMP-SMX). Percentage of MDR E.coli also increased over three years. Conclusion: There is an increase in antibiotic resistance amongst uropathogenic Escherichia coli over the past three years. Routine surveillance of resistance patterns should continue in the hospitals and antibiotic policy should be constantly updated. Judicious use of antibiotics is the need of the hour.

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Isolation of Campylobacter from human stool samples

Abstract: Isolation by culture was as sensitive as that of the PCR.

Cited by 23 publications

References 18 publications

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

Biofilm Formation by Histoplasma capsulatum in Different Culture Media and Oxygen Atmospheres

Virulence typing and antibiotic susceptibility profiling of thermophilic Campylobacters isolated from poultry, animal, and human species

Increasing antibiotic resistance amongst Uropathogenic E. coli isolated from inpatients with symptomatic UTI - a three year study

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