Isolation of Biofilm Producing Methicillin-Resistant Staphylococcus aureus from Hospitalized Orthopaedic Patients in Kano State, Nigeria

Oche, Dominic Agbo; Abdulrahim, Umar; Oheagbulem, Amaka Sylvia; Olayinka, Busayo Olalekan

doi:10.4314/njbas.v28i1.9

Cited by 3 publications

(3 citation statements)

References 21 publications

(22 reference statements)

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“…Even though S. aureus is a normal flora of the skin, the prevalence of S. aureus in this study is significantly high for a hospital setting despite the aseptic measures put in place during hospital procedures. The frequency in which S. aureus was isolated in this study is more than 14.8% reported by Oche et al [20]. Using oxacillinresistance screening agar (ORSA), this study had an MRSA prevalence of 31 (70.5%).…”

Section: Discussionmentioning

confidence: 52%

Detection of PBP2a and PVL Genes among Staphylococcus aureus and Their Methicillin-Resistant Strains Isolated from a Hospital in Sokoto Town

Ohaegbulem

Oche²,

Akinnibosun

et al. 2022

Microbes and Infectious Diseases

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Background: Methicillin-resistant Staphylococcus aureus (MRSA) has increasingly been implicated to be a causal organism of nosocomial infection. This study aimed at detecting PBP2a and PVL gene in Staphylococcus aureus (S. aureus) and MRSA isolated from in-patients and patients' caregivers in a Sokoto Hospital. Methods: This cross-sectional study involved 201 samples comprising 129 swabs from patients with surgical wounds on admission and 72 swabs from their caregivers/relatives. The samples were screened for S. aureus and MRSA using standard cultural methods, and they were further screened for PBP2a protein and PVL gene using a rapid PBP2a kit and polymerase chain reaction techniques respectively. Results: Findings showed a 21.9% recovery of S. aureus from the samples and a 70.5% prevalence rate of MRSA among the S. aureus isolates. S. aureus and MRSA were more prevalent in the wound swabs. The PBP2a protein was detected in 27.3% of the S. aureus isolated. It was interesting to note that 61.3% of the MRSA isolates lacked the PBP2a which is known to be an integral part of the mecA gene. The PBP2a was mostly detected in the isolates that came from the wound swabs. The PVL gene was detected in 32.3% of the S. aureus isolates and the PVL-positive isolates were all from wound swabs. Conclusions: The significant linkage of S. aureus isolates under study to methicillin resistance and the PVL gene is a call for caution. Therefore, the trends of MRSA in hospital-related infections should be recurrently investigated to avoid indiscriminate spread.

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Section: Discussionmentioning

confidence: 52%

Detection of PBP2a and PVL Genes among Staphylococcus aureus and Their Methicillin-Resistant Strains Isolated from a Hospital in Sokoto Town

Ohaegbulem

Oche²,

Akinnibosun

et al. 2022

Microbes and Infectious Diseases

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“…"Occurrence of biofilm forming strain reported in this study could be linked to the slow progression in wound healing process among orthopaedic patients. The biofilm matrix is known to be a vital factor in preventing antibiotics from reaching the infecting organisms within the matrix" [38], thereby conferring resistance on the bacteria within the biofilm matrix. Biofilm forming MRSA was identified consisting of high proportion 21.8%frompost-surgical wound followed by fracture wound sample recording 11.6%.…”

Section: Table 3 Multidrug Resistant (Mdr) Index Of Biofilm-forming M...mentioning

confidence: 99%

“…In this study, the Spearman's Rho Correlation statistical tool showed association between MRSA production and biofilm formation to be statistically significant at p< .05. This may be a sign that "due to the proximity or adherence of bacteria (MRSA) cells to each other within biofilms, resistant genes that confer resistance such as the mecAgene are easily transferred from one cell to other cells through HGT, thereby making the whole biofilm community resistant to methicillin and other antibiotics" [38].…”

Section: Table 3 Multidrug Resistant (Mdr) Index Of Biofilm-forming M...mentioning

confidence: 99%

Identification and Antibiotic Resistance Profile of Biofilm-forming Methicillin Resistant Staphylococcus aureus (MRSA) Causing Infection among Orthopedic Wound Patients

Peter

Okolo²,

Uzoeto³

et al. 2022

AJRIMPS

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Background and Objectives: The biofilm-forming ability of Methicillin-Resistant Staphylococcus aureus(MRSA) strains have demonstrated the involvement of MRSA biofilm in antibiotic resistance, recalcitrant and persistent infections in humans. Despite a deeper understanding of the biofilm-forming ability of MRSAstrain, it is still essential to extend the research on the identification and antibiotic resistance profile of biofilm-forming MRSA causing infection among orthopedic wound patients. Methodology: A total of three hundred and thirty (303) patient-isolate of non-repeatable Staphylococcus aureus strains were obtained during the period of 2021 until 2022 from fracture and post-surgical orthopedic wound patients with wound duration >2months at the National Orthopedic Hospital, Enugu (NOHE). S. aureus were identified using conventional microbiological cultures Technique followed by confirmation of MRSA strain through Brilliance MRSA 2 Agar. Antibiotic Susceptibility testing (AST) of biofilm-forming MRSA was performed using the Kirby–Bauer disk diffusion method and the results were interpreted using the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Multidrug Resistance (MDR) was determined for biofilm-forming MRSA. Result:Of the 303 isolate of S. aureus, MRSA strain accounted 86(28.4 %) and 78(25.7 %) from post-surgical wound and fracture wound respectively while biofilm forming MRSA was identified in 101(33.4%) MRSA strain consisting of high proportion 66(21.8 %) fromPost-surgical wound followed by fracture wound samples recording 35(11.6 %). Association between MRSA production and biofilm formation was considered statistically significant at P< .05. The proportion of biofilm-forming MRSA resistance to β-lactam accounted 71.4-100%, macrolide resistance recorded 65.7-92.4 %, lincosamideresistance 74.3-100 %, glycopeptide resistance proportion ranged from 62.8-100 % while low level of resistance to fluoroquinolones 19.7-42.9 % and Aminoglycoside 8.6-10.6 % was observed. Biofilm-forming MRSA isolate were MDR to one or more antibiotic antimicrobial agents in at least three categories withMDRIndex range ≥ 0.3 but majority of the isolate were 91.4% and 100% susceptible to Gentamicin and Imipenem. Conclusion: The invitro expression of biofilm formation among MRSA strain and their antibiotic resistance profile in this study makes them a potential threat and challenging pathogens with the ability to cause persistent infections in humans, especially among orthopedic wound patients. Thus the development of an antimicrobial stewardship program and regular detection of biofilm production is needed for timely intervention while judicious use of Imipenem and Gentamicin as a drug of choice for effective treatment of infection caused by biofilm-forming MRSA among orthopedic patients will avert the severity of infection. Further research of these sort should investigate the genotyping expression of a biofilm gene variant in other human diseases, different bacteria species, and orthopedic medical implant devices.

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Detection of rifampicin resistance in methicillin-resistant Staphylococcus aureus isolates from the medical centers at Ahmadu Bello University

Akpudo,

Oche,

Adikwu

et al. 2023

One Health Bulletin

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Objective: The aim of this study was to evaluate the prevalence of rifampicin resistance among methicillin-resistant Staphylococcus (S.) aureus (MRSA) isolates obtained from patients in two health facilities at Ahmadu Bello University, Zaria. Methods: A total of 200 non-duplicate presumptive Staphylococcal isolates were collected from the health facilities. The isolates were confirmed using mannitol salt agar, and biochemical tests were performed to identify S. aureus. The confirmed S. aureus isolates underwent antibiotic susceptibility tests, and the results were interpreted according to the guidelines provided by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Polymerase chain reaction (PCR) was used to investigate virulence and antibiotic resistance determinant, such as Spa, mecA, and rpoB. Results: This study revealed a S. aureus isolation rate of 22 (11.0%), and 18.2% of these S. aureus isolates were methicillin-resistant, with the highest prevalence of resistance (9.1%) found in wound swab. The results further showed that 75.0% of the MRSA isolates were resistant to rifampicin. The molecular analysis showed that all the isolates carried the virulence gene (Spa), with a prevalence of 10.0% for mecA and 20.0% for rpoB genes, respectively. Conclusions: This study underscores the prevalence of rifampicin resistance among MRSA isolates in medical centers, advocating for the development of alternative strategies to combat the current trends in antibiotic resistance and control of rifampicin-resistant MRSA.

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Isolation of Biofilm Producing Methicillin-Resistant Staphylococcus aureus from Hospitalized Orthopaedic Patients in Kano State, Nigeria

Cited by 3 publications

References 21 publications

Detection of PBP2a and PVL Genes among Staphylococcus aureus and Their Methicillin-Resistant Strains Isolated from a Hospital in Sokoto Town

Detection of PBP2a and PVL Genes among Staphylococcus aureus and Their Methicillin-Resistant Strains Isolated from a Hospital in Sokoto Town

Identification and Antibiotic Resistance Profile of Biofilm-forming Methicillin Resistant Staphylococcus aureus (MRSA) Causing Infection among Orthopedic Wound Patients

Detection of rifampicin resistance in methicillin-resistant Staphylococcus aureus isolates from the medical centers at Ahmadu Bello University

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