Isolation of Bacteriophages

Charante, Frits van; Holtappels, Dominique; Blasdel, Bob; Burrowes, Benjamin H.

doi:10.1007/978-3-319-40598-8_14-1

Cited by 15 publications

(15 citation statements)

References 124 publications

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“…Then, the phage suspension was filtered (0.45 µm) and propagated to increase the phage titer up to 10 9 –10 10 plaque forming units (pfu)/mL, according to Loessner and Busse (1990) [ 16 ]. For assuring the purification of the phage, this step was carried out 3 times consequently (3 step plaque purification assay) [ 17 ]. Only the purified ΦIZSAM-1 was further assessed for host range, genome/protein analysis and in vitro efficacy test.…”

Section: Methodsmentioning

confidence: 99%

Characterization and In Vitro Efficacy against Listeria monocytogenes of a Newly Isolated Bacteriophage, ΦIZSAM-1

et al. 2021

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Listeria monocytogenes is a bacterial pathogen responsible of listeriosis, a disease that in humans is often related to the contamination of ready-to-eat foods. Phages are candidate biodecontaminants of pathogenic bacteria thanks to their ability to lyse prokaryotes while being safe for eukaryotic cells. In this study, ɸIZSAM-1 was isolated from the drain-waters of an Italian blue cheese plant and showed lytic activity against antimicrobial resistant Listeria monocytogenes strains. This phage was subjected to purification and in vitro efficacy tests. The results showed that at multiplicities of infection (MOIs) ≤ 1, phages were able to keep Listeria monocytogenes at low optical density values up to 8 h, with bacterial counts ranging from 1.02 to 3.96 log10 units lower than the control. Besides, ɸIZSAM-1 was further characterized, showing 25 principal proteins (sodium dodecyl sulfate polyacrylamide gel electrophoresis profile) and a genome of approximately 50 kilo base pairs. Moreover, this study describes a new approach to phage isolation for applications in Listeriamonocytogenes biocontrol in food production. In particular, the authors believe that the selection of phages from the same environments where pathogens live could represent a new approach to successfully integrating the control measures in an innovative, cost effective, safe and environmentally friendly way.

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Section: Methodsmentioning

confidence: 99%

Characterization and In Vitro Efficacy against Listeria monocytogenes of a Newly Isolated Bacteriophage, ΦIZSAM-1

et al. 2021

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“…Bacteriophage Mano was isolated by plaque purification ( 2 ) from a Michigan topsoil sample by plating on nutrient agar lawns of Achromobacter xylosoxidans grown at 37°C. Genomic DNA was purified using the Wizard DNA cleanup kit (Promega) as previously described ( 3 ) and prepared as Illumina TruSeq libraries.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Achromobacter xylosoxidans Myophage Mano

Bartz

Yao

et al. 2021

Microbiol Resour Announc

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“…Phage Salva was isolated in 2019 from a wastewater sample collected in Cincinnati, Ohio, by enriching the sample using a multidrug-resistant S. maltophilia clinical isolate obtained from human sputum in Decatur, GA, as the host strain aerobically grown at 30°C in nutrient broth (BD), followed by plaque purification ( 3 , 4 ). To purify the genomic DNA, the modified Promega Wizard DNA cleanup kit protocol was used ( 5 ).…”

Section: Announcementmentioning

confidence: 99%