“…Examples include hexamer [16], [17], [20], [26], [30], [34], [35], [36], [37], [38], octamer [19], [22], [23], [39], [40], [41], [42], nonamer [24], [43], [44], [45] and decamer random 3′ annealing parts [46]. Stangegaard and colleagues [47] studied the impact of different random primers (without 5′ specific amplification tag sequence) on the yield and quality of synthesized cDNA, concluding that reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA compared with hexa- and nonamers.…”