Isolation of Arcobacter spp from poultry carcasses, in Brazil

Abstract

Fourty eight isolates of Arcobacter spp were obtained from 37 poultry carcasses, from abattoir, among 80 carcasses examined. Attempts for culturing were made from the skin and muscle, resulting on 25 positive cultures from muscle and 23 from skin. Classification was achieved by phenotypic characterization and PCR and multiplex PCR, resulting 41 samples of Arcobacter butzleri and 07 Arcobacter sp. This is the first report on the occurrence of Arcobacter in animal carcasses in Brazil.

Isolation of Arcobacter spp from the milk of dairy cows in Brazil

“…The forward and reverse primers used in this study were ARC-1: 5´-AGA GAT TAG CCT GTA TTG TAT C -3´ ARC-2: 5´-TAG CAT CCC CGC TTC GAA TGA -3´. Specific primers for A. butzleri were ARC-5: 5´-TTC GCT TGC GCT GAC AT -3´ and ARC-6: 5´CTA TCC AGC GTA GAA GAT G -3´, amplifying a 686 bp fragment from the gene 23S rRNA (HARMON & WESLEY, 1996;OLIVEIRA et al, 2001).…”

“…A. cryaerophilus have been isolated from aborted pig fetuses from some countries, including Brazil (SCHROEDER-TUCKER et al, 1996;OLIVEIRA et al, 1997;1999). A. butzleri have been detected from enteritis in humans (LERNER et al, 1994;LAWERS et al, 1996;LAU et al, 2002;FERNANDEZ et al, 2004;VANDENBERG et al, 2004) and also the microorganisms were isolated from poultry and pig carcasses (RIDSDALE et al, 1998;OLIVEIRA et al, 2001;.…”

Isolation, identification and genotypic and phenotypic characterization of >i<Arcobacter>/i< spp. isolated from Minas frescal cheese and raw milk

“…In Brazil, there are specific studies about the occurrence of Arcobacter in in slaughterhouses and processing plants of chicken and pig. There are some studies concerning the occurrence of bacteria in retail products and commercialization points (Oliveira, 2016;Oliveira et al, 2001), but no publications are available in dairy products . analyzed 188 raw bovine milk samples collected from 11 dairy farms located in different cities of Rio Grande do Sul, Brazil, and results showed a prevalence of 5%.…”