Isolation of arbuscular mycorrhizal fungi and Azotobacter chroococcum from local litchi orchards and evaluation of their activity in the air-layers system

Sharma, Samriti; Kumar, Pramod; Raj, Himanshu; Bhardwaj, S. D.

doi:10.1016/j.scienta.2009.07.019

Cited by 37 publications

(12 citation statements)

References 21 publications

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“…Nitrogen fixing Azotobacter strains were isolated by serial dilution agar plate method and 1ml of serially diluted soil suspensions from 10 -3 , 10 -4 , 10 -5 and 10 -6 dilutions were inoculated onto Jenson's N-free media (Ahmad et al, 2008;Sharma et al, 2009). The plates were maintained in triplicates for higher accuracy of the results.…”

Section: Isolation and Identification Of Azotobacter Speciesmentioning

confidence: 99%

“…This report clearly indicates that the isolates has capacity to increase the vigor of the plant and resistant to all the concentrations of pesticides. Sharma et al (2009) reported A. chroococcum strain increased the litchi root and shoot length by 81.39% and was isolated from orchard soils . Similarly , Patil ( 2011 ) reported the impact of Azotobacter culture on root and shoot length of groundnut (38.88 to 49.88) and gram (27.65 to 36.35) seeds.…”

Section: Effect Of Pesticides On Bioassay Activitymentioning

confidence: 99%

“…Many species of Pseudomonas, Bacillus and Azotobacter can grow and survive at extreme environmental conditions (Jimenez et al 2011). Among them, the genus Azotobacter are common nitrogen fixing bacterium found in agricultural soils playing different beneficial roles by secreting vitamins, amino acids, sidephores and auxins (Aquilanti et al, 2004;Sharma et al, 2009). Azotobacter produces indole acetic acid (IAA), gibberllic acid (GA) which are important plant growth hormones and these hormones will helps in seed germination and plant growth considerably (Asma et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of Pesticides on PGPR Activity of Azotobacter sp. Isolated from Pesticide Flooded Paddy Soils

Chennappa¹,

Adkar-Purushothama²,

Naik³

et al. 2014

GJAS

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Azotobacter strains were isolated from paddy soils by serial dilution agar plate method.The colonies were glistening; smooth, slimy, brown to black in morphology on Jenson's N-free agar plates and the cells were Gram-negative bacteria. Biochemically, they were positive for indole production, citrate, catalase and Voges Proskauer test. Further, Nif gene sequence results revealed the presence of seven Azotobacter species. The effect of 1, 3 and 5% pesticides concentration viz., pendimethalin, chloropyrifos, glyphosate and phorate on nitrogen fixation, indole acetic acid, gibberllic acid, phosphate solubilization and bioassay of Azotobacter sp. were studied. Among all the species, GVT-1 strain was found to fix nitrogen at a maximum of 30µgN2 ml -1 day -1 , produced highest quantity gibberllic acid (10µg25ml -1 ) and able to solubilize the phosphate at the rate of 9.8cm by forming the halo zone which was supplemented with 5% phorate. Similarly, GVT-1 strain produced a highest amount of indole acetic acid (31.8µgml -1 ) in 5% pendimethalin. Further bioassay activities of GVT-1 strain found efficient in increasing the root, shoot length and vigor of the plant. From these results it is clear that the Azotobacter sp. not only produces plant growth promoting substances but is also resistant to different pesticides and is not affected by the bacterial activity.

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Section: Isolation and Identification Of Azotobacter Speciesmentioning

confidence: 99%

Section: Effect Of Pesticides On Bioassay Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Pesticides on PGPR Activity of Azotobacter sp. Isolated from Pesticide Flooded Paddy Soils

Chennappa¹,

Adkar-Purushothama²,

Naik³

et al. 2014

GJAS

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“…pH of the samples was determined in soil and water suspension in a 1:2 ratio and dry weight was determined by the weight loss of 10 g after drying in an oven at 105 C for 24 h. [18,19] The heavy metal concentration in the industrial samples was assessed in the previous study ( Table 2). [6] Eight out of 20 samples collected from the steelworks were characterized by the concentration of heavy metals that exceeded the admissible values given in the Regulation of the Minister of Environment of September 9, 2002 (Zn, Cd, Pb, Cu and Cr).…”

Section: Methodsmentioning

confidence: 99%

The molecular marker-based comparison ofAzotobacterspp. populations isolated from industrial soils of Cracow-Nowa Huta steelworks (southern Poland) and the adjacent agricultural soils

Lenart‐Boroń¹,

Wolny-Koładka²,

Boroń

et al. 2014

Journal of Environmental Science and Health, Part A

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The occurrence of Azotobacter spp., which has beneficial effects on plant development, is related to various soil properties, such as pH and fertility. This study evaluated the prevalence of Azotobacter spp. in industrial (H) and agricultural soils (P) in Nowa Huta, Cracow and determined the phenotypic and genetic diversity of these bacteria. The examined bacteria were present in 40% of H and in 50% of P soils. Taxonomic identification of the bacterial isolates indicated the presence of three species--A. salinestris, A. chroococcum and A. vinelandii. The genetic diversity, determined using two fingerprinting methods--Random Analysis of Polymorphic DNA (RAPD) and Rep-PCR (BOX) revealed high level of population diversity. In AMOVA analysis most of diversity was attributed to within-population variation (76-85%), and only 3.78-6.18% was associated with among-group H and P variation. Global test of differences revealed distinct population structure within bacterial strains isolated from H and P areas only for BOX markers (Fst = 0.05732, P = 0.00275). Phenetic analyses: UPGMA and DCA better discriminated H and P groups based on RAPD data. Both BOX and RAPD methods provided an insight into the genetic complexity of Azotobacter spp. variation in soils of different land-use types.

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“…Although this method is strongly influenced by operator skill, it could provide sufficient information to evaluate the mycorrhizal colonization level. In fact, the gridline intersect method has been extensively used in many works to assess and quantify root colonization of mycorrhizal fungi (Sharma et al, 2009;Sharma et al, 2011;Sharma et al, 2012;Singh et al, 2013).…”

Section: Optical Microscopymentioning

confidence: 99%

Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence

2020

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Isolation of arbuscular mycorrhizal fungi and Azotobacter chroococcum from local litchi orchards and evaluation of their activity in the air-layers system

Cited by 37 publications

References 21 publications

Impact of Pesticides on PGPR Activity of Azotobacter sp. Isolated from Pesticide Flooded Paddy Soils

Impact of Pesticides on PGPR Activity of Azotobacter sp. Isolated from Pesticide Flooded Paddy Soils

The molecular marker-based comparison ofAzotobacterspp. populations isolated from industrial soils of Cracow-Nowa Huta steelworks (southern Poland) and the adjacent agricultural soils

Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence

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