Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence

Romano, Ida; Ventorino, Valeria; Pepe, Olimpia

doi:10.3389/fpls.2020.00006

Cited by 83 publications

(49 citation statements)

References 111 publications

(141 reference statements)

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“…Rhizosphere samples were collected according to Romano et al (2020) from wheat plants grown, under drought stress and nitrogen deficiency, in a greenhouse at the experimental station of the University of Naples Federico II (Bellizzi, Italy; 43°31′N, 14°58′E, 60 m a.s.l.). Ten grams of samples were shaken for 30 min in 90 mL of quarter strength Ringer’s solution (Oxoid, Milan, Italy) containing tetrasodium pyrophosphate (16% w/v) as previously described ( Ventorino et al, 2012a ).…”

Section: Methodsmentioning

confidence: 99%

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

Romano

Ventorino

Ambrosino³

et al. 2020

Front. Microbiol.

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The use of beneficial microbes as inoculants able to improve fitness, growth and health of plants also in stress conditions is an attractive low-cost and eco-friendly alternative strategy to harmful chemical inputs. Thirteen potential plant growth-promoting bacteria were isolated from the rhizosphere of wheat plants cultivated under drought stress and nitrogen deficiency. Among these, the two isolates TL8 and TL13 showed multiple plant growth promotion activities as production of indole-3-acetic acid (IAA), siderophores, ammonia, and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase production, the ability to solubilize phosphate as well as exerted antimicrobial activity against plant pathogens as Botrytis spp. and Phytophthora spp. The two selected strains were identified as Kosakonia pseudosacchari by sequencing of 16S rRNA gene. They resulted also tolerant to abiotic stress and were able to efficiently colonize plant roots as observed in vitro assay under fluorescence microscope. Based on the best PGP properties, the strain K. pseudosacchari TL13 was selected to develop a new microbial based formulate. A sustainable and environmentally friendly process for inoculant production was developed using agro-industrial by-products for microbial growth. Moreover, the application of K. pseudosacchari TL13- based formulates in pot experiment improved growth performance of maize plants.

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Section: Methodsmentioning

confidence: 99%

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

Romano

Ventorino

Ambrosino³

et al. 2020

Front. Microbiol.

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“…In the past decade, the advances of High-Throughput Sequencing (HTS) approaches have been extensively used to investigate bacterial diversity in various systems [23,24]. These approaches have revealed a vast inter-and intraspecies diversity of 16S rRNA gene sequences and have suggested the existence of highly complex functions and biotic interactions within the microbiomes of plants [25], previously undetected with classical culture techniques [26]. The HTS tools have been used to assess and reveal the whole bacterial diversity of the rhizosphere [27] and the phyllosphere [28] of various plants, indicating that the phyllospheric communities were overall less diverse than the rhizospheric ones [15].…”

Section: Introductionmentioning

confidence: 99%

Bacterial Communities in the Rhizosphere and Phyllosphere of Halophytes and Drought-Tolerant Plants in Mediterranean Ecosystems

et al. 2020

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The aim of the study was to investigate the bacterial community diversity and structure by means of 16S rRNA gene high-throughput amplicon sequencing, in the rhizosphere and phyllosphere of halophytes and drought-tolerant plants in Mediterranean ecosystems with different soil properties. The locations of the sampled plants included alkaline, saline-sodic soils, acidic soils, and the volcanic soils of Santorini Island, differing in soil fertility. Our results showed high bacterial richness overall with Proteobacteria and Actinobacteria dominating in terms of OTUs number and indicated that variable bacterial communities differed depending on the plant’s compartment (rhizosphere and phyllosphere), the soil properties and location of sampling. Furthermore, a shared pool of generalist bacterial taxa was detected independently of sampling location, plant species, or plant compartment. We conclude that the rhizosphere and phyllosphere of native plants in stressed Mediterranean ecosystems consist of common bacterial assemblages contributing to the survival of the plant, while at the same time the discrete soil properties and environmental pressures of each habitat drive the development of a complementary bacterial community with a distinct structure for each plant and location. We suggest that this trade-off between generalist and specialist bacterial community is tailored to benefit the symbiosis with the plant.

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“…Another important issue will be to assess the persistence and competitiveness of beneficial microbes when there is a positive interaction with plants, particularly when microbial inoculants are used to improve plant performance ( Romano et al , 2020 ). In parallel, fundamental research needs to be conducted to characterize the interactions regarding N acquisition between plants and beneficial microorganisms at the physiological and molecular levels.…”

Section: Discussionmentioning

confidence: 99%

Beneficial soil-borne bacteria and fungi: a promising way to improve plant nitrogen acquisition

Dellagi

Quilleré

Hirel

2020

Journal of Experimental Botany

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Nitrogen (N) is an essential element for plant productivity, thus, it is abundantly applied to the soil in the form of organic or chemical fertilizers that have negative impacts on the environment. Exploiting the potential of beneficial microbes and identifying crop genotypes that can capitalize on symbiotic associations may be possible ways to significantly reduce the use of N fertilizers. The best-known example of symbiotic association that can reduce the use of N fertilizers is the N2-fixing rhizobial bacteria and legumes. Bacterial taxa other than rhizobial species can develop associative symbiotic interactions with plants and also fix N. These include bacteria of the genera Azospirillum, Azotobacter, and Bacillus, some of which are commercialized as bio-inoculants. Arbuscular mycorrhizal fungi are other microorganisms that can develop symbiotic associations with most terrestrial plants, favoring access to nutrients in a larger soil volume through their extraradical mycelium. Using combinations of different beneficial microbial species is a promising strategy to boost plant N acquisition and foster a synergistic beneficial effect between symbiotic microorganisms. Complex biological mechanisms including molecular, metabolic, and physiological processes dictate the establishment and efficiency of such multipartite symbiotic associations. In this review, we present an overview of the current knowledge and future prospects regarding plant N nutrition improvement through the use of beneficial bacteria and fungi associated with plants, individually or in combination.

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Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence

Cited by 83 publications

References 111 publications

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

Development and Application of Low-Cost and Eco-Sustainable Bio-Stimulant Containing a New Plant Growth-Promoting Strain Kosakonia pseudosacchari TL13

Bacterial Communities in the Rhizosphere and Phyllosphere of Halophytes and Drought-Tolerant Plants in Mediterranean Ecosystems

Beneficial soil-borne bacteria and fungi: a promising way to improve plant nitrogen acquisition

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