Isolation of Antibody-like Gammaglobulin from Lupus Glomeruli

Freedman, Paul D.; Markowitz, A. S.

doi:10.1136/bmj.1.5286.1175

Cited by 47 publications

(14 citation statements)

References 6 publications

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“…The infrequent demonstration of in %ivo nuclear y-globulin or fl10-globulin in the kidneys of our patients argues against a major autoaggressive pathogenetic role in renal lesions of antinuclear antibodies, although these antibodies may combine with nuclei of damaged cells. DNA and other tissue antigens may be components of antigen-antibody complexes localized in the kidney (36). Attempts to localize DNA antigen with human sera containing anti-DNA antibodies have been unsuccessful (32).…”

Section: Discussionmentioning

confidence: 99%

Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus.

Paronetto¹,

Koffler²

1965

J. Clin. Invest.

125

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Section: Discussionmentioning

confidence: 99%

Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus.

Paronetto¹,

Koffler²

1965

J. Clin. Invest.

125

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“…In the present study, the postmortem kidneys of six patients with systemic lupus erythematosus (SLE), and six control patients 569 4, J.T., 10-year-old Negro male Proteinuria, RBC, leukocytes, Proliferative and membranous glomerulitis with SLE and casts in sediment for 1 year. 5, E.S., 52-year-old Negro female RBC, leukocytes in sediment, Membranous glomerulitis with "wire loops" with SLE severe proteinuria, and proand focal fibrinoid necrosis gressive azotemia for 2 months.…”

Section: Introductionmentioning

confidence: 99%

Immunopathologic Studies of Systemic Lupus Erythematosus. II. Antinuclear Reaction of γ-Globulin Eluted from Homogenates and Isolated Glomeruli of Kidneys from Patients with Lupus Nephritis *

Krishnan¹,

Kaplan²

1967

J. Clin. Invest.

202

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Summary. The yG-globulin eluted at acid pH from kidney cortex homogenates and isolated glomeruli of five of six patients with lupus nephritis was found to exhibit antinuclear activity, which was not dependent on presence of fresh human serum. Specificity, as demonstrated by absorption of antinuclear activity, was related to nucleoprotein in three glomerular acid eluates and to DNA in two acid eluates as well as in a deoxyribonuclease digest of disrupted glomeruli in one patient. Antinuclear activity was not found in acid eluates of kidneys from two patients with chronic liver disease and chronic discoid lupus, respectively, and one with lupus nephritis. These patients had a low titer of serum antinuclear factor and lesser amounts of kidney bound immunoglobulins. The presence of antinuclear activity in eluates of kidneys appeared to correlate with the amount of glomerular bound immunoglobulin and the level of antinuclear antibodies in serum. These findings suggest that in lupus nephritis, part of the glomerular bound immunoglobulin is derived from serum antinuclear factors possibly deposited as immune complexes. IntroductionThe definition of immunoglobulin deposits in lupus kidneys has been approached by techniques applicable to dissociation of antigen-antibody complexes. Sections of lupus nephritis kidneys washed at acid pH (3.0 to 3.2) were found to lose considerable staining for y-globulin in glomerular and vas-

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“…The presence of immunoglobulins and complement has been demonstrated in glomerular lesions by immunofluorescence techniques (4)(5)(6)(7), and antinuclear antibodies have been found in eluates from the glomeruli (8)(9)(10)(11). Fluorescein-labeled, highly purified antibody to DNA obtained from the serum of a lupus patient has localized in the glomeruli of two patients with SLE nephritis.…”

mentioning

confidence: 99%

Localization of fluorescein-labeled antinucleoside antibodies in glomeruli of patients with active systemic lupus erythematosus nephritis

Andres¹,

Accinni²,

Beiser³

et al. 1970

J. Clin. Invest.

129

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A B S T R A C T Renal tissues from two groups of patients were studied with fluorescein-labeled (Fl-) antibodies (Abs) to immunoglobulins, complement, and antibodies prepared in rabbits against BSA conjugate of 5-methyluridine (T) and cytidine (C), the latter two of which react specifically with denatured DNA. The first group consisted of 13 SLE patients, and the second consisted of 53 patients with non-SLE nephropathies. The data obtained from the two groups of patients were used for comparison, and they showed the following:(a) Fl-Abs to immunoglobulins and complement were bound in the glomeruli of tissues from all patients with active SLE glomerulonephritis characterized by deposits of foreign material in glomerular capillary walls (GCW). The fluorescent pattern was granular, corresponding to the distribution of the glomerular deposits, as seen by electron microscopy. Fl-Abs reactive with thymine and cytosine were bound in the GCW of eight of the nine patients with active SLE glomerulonephritis and showed the same granular distribution. The capacity of these latter Fl-Abs to stain the GCW was removed by absorption with the homologous antigen or denatured DNA.(b) Fl-Abs to immunoglobulins, complement, and pyrimidine bases of DNA did not react with the GCW of two SLE patients without clinical and histologic evidence of glomerulonephritis or with the sclerotic glomeruli of two uremic patients with chronic "burned out" lupus nephritis.(c) The glomeruli of 47 of the 53 patients with other nephropathies bound Fl-Abs to immunoglobulins and

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Isolation of Antibody-like Gammaglobulin from Lupus Glomeruli

Cited by 47 publications

References 6 publications

Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus.

Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus.

Immunopathologic Studies of Systemic Lupus Erythematosus. II. Antinuclear Reaction of γ-Globulin Eluted from Homogenates and Isolated Glomeruli of Kidneys from Patients with Lupus Nephritis *

Localization of fluorescein-labeled antinucleoside antibodies in glomeruli of patients with active systemic lupus erythematosus nephritis

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