Isolation of Anaerobic Bacteria from Human Gingiva and Mouse Cecum by Means of a Simplified Glove Box Procedure1

Aranki, Alexander; Syed, Salam A.; Kenney, E. Barrie; Freter, Rolf

doi:10.1128/aem.17.4.568-576.1969

Cited by 243 publications

(123 citation statements)

References 11 publications

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“…This dental plaque was harvested under a stream of nitrogen gas, placed into a prereduced transport fluid, and immediately placed into an anaero bic glove box. 16 These plaque samples were then dis persed with an ultrasonic probe and a bacterial count made using a Petroff-Hauser counting chamber viewed at x 1,000 magnification. 16 Serial dilutions were made and plaque bacteria were plated on modified Huntoons media.…”

Section: Anaerobic Bacteria In Dental Plaquementioning

confidence: 99%

The Effect of Cigarette Smoking on Anaerobiosis in the Oral Cavity

Kenney

Saxe

Bowles

1975

Journal of Periodontology

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Nineteen young adult male smokers were compared to 19 adult male nonsmokers to determine if cigarette smoking resulted in any changes to anaerobic bacteria in dental plaque, to intraoral oxidation-reduction potential (Eh) levels, or to intraoral pH. There was no statistical difference between smokers and nonsmokers in the proportion of anaerobic bacteria found in dental plaque. There were no significant differences between smokers and nonsmokers with respect to the resting Eh in the floor of the mouth nor in the Eh in the region of buccal surface of the upper molars. The smoking of one cigarette resulted in a dramatic fall in Eh in both intraoral regions and this occurred in all subjects, both smokers and nonsmokers. The magnitude of this fall in Eh was similar in both groups. There were also uniform increases in pH, but these pH changes were much smaller in magnitude.

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Section: Anaerobic Bacteria In Dental Plaquementioning

confidence: 99%

The Effect of Cigarette Smoking on Anaerobiosis in the Oral Cavity

Kenney

Saxe

Bowles

1975

Journal of Periodontology

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“…This may not be recognized in the sample since determination of the location ofthe apical plaque border and sampling itself through the pocket depend on the tactile sensation only. Aranki et al (1969) mentioned the distortions in counts of the original microflora due to sampling technique. Henry et al (1974) used a sterile plastic micropipette with slight negative pressure to avoid contamination with supragingival plaque.…”

Section: Discussionmentioning

confidence: 99%

False results associated with darkground microscopy of subgingival plaque

Omar

Newman

1986

J Clinic Periodontology

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Abstract. This study considers false results which may arise due to problems in the preparation or examination of specimens for darkground microscopy of subgingival plaque. Subgingival plaque samples obtained with a sterile curette were placed in 0.1–0.3 ml sterile full or 1/4 strength Ringer's solution: 0.85% saline, 1% gelatin in 0.85% saline, formal saline or pyrogen‐free water for injection. Test slides were prepared from the original dispersion, and control slides from the corresponding sterile solution. Optimal dispersion solution, syringe dispersion frequency and the effect on motility of delay in processing samples were tested. Slides were also prepared from dispersions of 11 representative subgingival “periodontopathic” organisms. Problems in sampling included variability in counts between sites with comparable pocket depths, contamination of the sample and reduction of the sample volume after scaling. Problems in dispersion included contamination, uneven distribution of the different morphotypes and destruction of delicate organisms. Problems in slide preparation included slide contamination, limitation in the number of samples that can be assessed by one examiner at a given time without loss of activity of motile cells, and preparation of a cell monolayer. Problems in identification and counting included confusion of Brownian movements with motility, coccoid particles with cocci, spirochetes with campylobacter, flagella with flagella‐like structures, size of cocci, counting of fragmented spirochetes and non‐motile flagellated organisms and motile cells, and also bias in counting. Problems in morpbotype grouping included the observation that many (10 of the 11 representative) periodontitis‐related organisms were in the non‐motile groups and not all cells of the motile species (Campylobacter, Capnocytophaga) showed motility. The results indicate that each stage of subgingival plaque darkground microscopy, sampling, dispersion, slide preparation, counting, morphotype grouping and interpretation may lead to false results if not representative or reproducible. Procedures are suggested for the minimisation of problems in the preparation and examination of subgingival plaque specimens for darkground microscopy.

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“…Faecal samples were collected, diluted and plated into appropriate agar plates. They were exposed to air for 1 h to preselect for microorganisms that were not extremely oxygen sensitive [20], prior to incubation at 37³C for 3 days under anaerobic conditions in a Freter anaerobic glove box [21]. Antibiotics (Coger and Sigma) were added as required at ¢nal concentrations of 10 Wg ml 3I tetracycline (Tc), 100 Wg ml 3I rifampicin (Rif), 10 Wg ml 3I clindamycin (Cc), 100 Wg ml 3I erythromycin (Em), 140 Wg ml 3I neomycin (Neo), 50 Wg ml 3I Nal, 100 Wg ml 3I streptomycin (Sm), 50 Wg ml 3I kanamycin (Kn) and 25 Wg ml 3I ampicillin (Ap).…”

Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

Transfer of the shuttle vector pRRI207 between Escherichia coli and Bacteroides spp. in vitro and in vivo in the digestive tract of axenic mice and in gnotoxenic mice inoculated with a human microflora

Garrigues-Jeanjean

1999

FEMS Microbiology Ecology

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Transfer of the shuttle vector pRRI207 mediated by the helper plasmid pRK2013 from Escherichia coli to Bacteroides spp. was possible in vitro and in vivo in the digestive tract of axenic mice associated with Bacteroides uniformis 1004 or Bacteroides vulgatus of human origin. In vivo, transfer frequencies were nearly identical for B. uniformis (2U10 3U ) and B. vulgatus (4U10 3U ) and the transconjugant strains of B. uniformis and B. vulgatus became established in the digestive tract of mice at densities ranging from 10 P^1 0 Q to 10 R CFU g 3I of faeces, respectively. Transfer from E. coli to Bacteroides strains in gnotoxenic mice associated with human faecal flora (HFF) was not successful. Transconjugant-like clones appeared among the HFF of gnotoxenic mice after they were inoculated with B. uniformis TBUA, a transconjugant strain of B. uniformis 1004 obtained from triparental mating and which harboured the shuttle vector. Hybridisation showed that the shuttle vector pRRI207 was not present in these clones, and it is suggested that they could result from the transfer of a conjugative transposon ERL contained in B. uniformis 1004. Moreover, clones believed to have lost the shuttle vector hybridised with a specific probe to B. thetaiotaomicron and therefore did not originate from B. uniformis TBUA. z

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Isolation of Anaerobic Bacteria from Human Gingiva and Mouse Cecum by Means of a Simplified Glove Box Procedure1

Cited by 243 publications

References 11 publications

The Effect of Cigarette Smoking on Anaerobiosis in the Oral Cavity

The Effect of Cigarette Smoking on Anaerobiosis in the Oral Cavity

False results associated with darkground microscopy of subgingival plaque

Transfer of the shuttle vector pRRI207 between Escherichia coli and Bacteroides spp. in vitro and in vivo in the digestive tract of axenic mice and in gnotoxenic mice inoculated with a human microflora

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