Isolation of an asparagus intracellular <i>PR</i> gene (<i>AoPR1</i>) wound‐responsive promoter by the inverse polymerase chain reaction and its characterization in transgenic tobacco

Warner, Simon; Scott, Rod; Draper, John

doi:10.1046/j.1365-313x.1993.t01-11-00999.x

Cited by 84 publications

(75 citation statements)

References 44 publications

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“…The element was positioned next to the Ac-Tpa gene fused to the asparagus (Asparagus officinalis) Pr1 (AoPr1) promoter. By rapidly responding to both wounding and infection (Warner et al, 1993), the AoPr1 promoter was expected to drive early Tpa gene expression and allow Ds excision prior to T-DNA degradation. To select against stable T-DNA integration, an expression cassette for the negative selectable marker gene cytosine deaminase (codA) was placed on the other side of Ds.…”

Section: Production Of Transformed Plantsmentioning

confidence: 99%

Transposition-Based Plant Transformation

Hua

Rommens

2006

Plant Physiology

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Agrobacterium T-DNAs were used to deliver transposable Dissociation (Ds) elements into the nuclei of potato (Solanum tuberosum) cells. A double-selection system was applied to enrich for plants that only contained a transposed Ds element. This system consisted of a positive selection for the neomycin phosphotransferase (nptII) gene positioned within Ds followed by a negative selection against stable integration of the cytosine deaminase (codA) gene-containing T-DNA. Sixteen of 29 transgenic plants were found to contain a transposed element while lacking any superfluous T-DNA sequences. The occurrence of this genotype indicates that Ds elements can transpose from relatively short extrachromosomal DNA molecules into the plant genome. The frequency of single-copy Ds transformation was determined at 0.3%, which is only about 2.5-fold lower than the potato transformation frequency for backbone-free and single-copy T-DNAs. Because of the generally high expression levels of genes positioned within transposed elements, the new transformation method may find broad applicability to crops that are accessible to Agrobacterium T-DNA transfer.

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Section: Production Of Transformed Plantsmentioning

confidence: 99%

Transposition-Based Plant Transformation

Hua

Rommens

2006

Plant Physiology

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“…Recent work in this laboratory on AoPR-1, an intracellular PR protein of Asparagus officinalis (Warner et al, 1992(Warner et al, , 1993) has shown that it is possible to separate the effects of H202 and SA on the induction of defence responses (Mur et al, manuscript submitted). As noted previously (Figure 3a), H202 is a relatively weak inducer of endogenous PR-la expression.…”

Section: Local Expression Of Pr Proteins Is Induced To Suboptimal Levmentioning

confidence: 99%

“…Transgenic tobacco lines used were all T 2 generation derived as described by Warner et al (1993). Chemical treatment of tobacco leaf material involved either injection of intercellular leaf spaces in planta with a 2 ml syringe fitted with a 27G needle, or utilized 1.5 cm diameter leaf discs cored with a cork-borer and treated in Multi-Well dishes (Nunc).…”

Section: Plant Growth Conditions and Treatmentsmentioning

confidence: 99%

“…PR-la-GUS tobacco variety Samsun NN lines 3, 4 and 13 were selected as having 'high', 'medium' and 'low' GUS expression, respectively, in response to treatment with 1 mM SA. AoPR-1-GUS tobacco variety SR1 lines 1 and 14 (Warner et aL, 1993) and Samsun NN line L with varying wound-inducible GUS activities were selected as representative of AoPR-1. Fluorimetric determination of GUS activities followed the Jefferson etal.…”

Section: Gus Assaysmentioning

confidence: 99%

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Hydrogen peroxide does not function downstream of salicylic acid in the induction of PR protein expression

et al. 1995

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SummaryThe roles of salicylic acid (SA) and H202 in the induction of PR proteins in tobacco have been examined. Studies were conducted on wild-type tobacco and plants engineered to express a bacterial salicylate hydroxylase capable of metabolizing SA to catechol (SH-L plants). Wildtype and PR-la-GUS-transformed plants express PR-la following challenge with Pseudomonas syringae pathovar syringae, SA or 2,6-dichloro-isonicotinic acid (INA). In contrast, SH-L plants failed to respond to SA but did express PR-la following INA treatment. H202 and the irreversible catalass inhibitor 3-amino-l,2,4-triazole (3-AT) were found to be weak inducers of PR-la expression (relative to SA) in wild-type tobacco but were unable to induce PR-la in SH-L plants, suggesting that the action of these compounds depends upon the accumulation of SA. A model has been proposed suggesting that SA binds to and inhibits a catalase inducing an increase in H202 leading to PR protein expression. Catalase activity has been measured in tobacco end no significant changes in activity following infection with P. syringae pv. syringee were detected. Furthermore, inhibition of catalass activity in vitro in plant extracts requires pre-incubation and only occurs at SA concentrations above 250 I~M. Leaf disks preincubated with I mM SA do accumulate SA to these levels and PR-la is efficiently induced but there is no apparent inhibition of catalass activity. It is also shown that a SAresponsive gene, PR-le, and a H202-sensitive gene, AoPR-1, are both relatively insensitive to 3-AT suggesting that induction of these genes is unlikely to be due entirely to inhibition of an endogenous catalase.

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“…Characterization of PR-10 proteins and development of transgenic plants overexpressing PR-10 proteins is important step in this direction. Table 1 lists the PR-10 genes which have been used to develop transgenic plants in different crop species [95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110]. Multi-location field trials of transgenic plants expressing PR-10 will likely be next step for further evaluation.…”

Section: Pr-10 Proteins: a Resource For Crop Improvementmentioning

confidence: 99%

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

Jain¹

2015

APAR

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One of the most represented group of Pathogenesis Related (PR) genes are those of the PR-10 class. PR-10 proteins are members of multi genic family, and they often occur in clusters at specific loci following gene duplication and amplification events. To date, large number of PR-10 genes have been cloned and characterized in different species in response to abiotic and biotic stress. This review is focused on recent studies that have described the role, distribution and structure of PR-10 genes in plant genomes. Recent findings have provided insights into the functional roles of PR-10 proteins as ribonuclease, as cytokinin-specific binding proteins, a mammalian lipid transport and plant abscisic acid (ABA) receptor proteins, or as enzyme, (S)-norco claurine synthase. PR-10 proteins are differentially expressed in the presence of different signaling molecules, biotic stresses such as fungal, viral and bacterial pathogens and a number of abiotic stresses. The possibility to use this knowledge for genetic improvement of plant resistance to pathogens through classical breeding approach or transgenic technology is discussed.

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Isolation of an asparagus intracellular PR gene (AoPR1) wound‐responsive promoter by the inverse polymerase chain reaction and its characterization in transgenic tobacco

Cited by 84 publications

References 44 publications

Transposition-Based Plant Transformation

Transposition-Based Plant Transformation

Hydrogen peroxide does not function downstream of salicylic acid in the induction of PR protein expression

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

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