Isolation of an antileishmanial and antitrypanosomal flavanone from the leaves of Baccharis retusa DC. (Asteraceae)

Grecco, Simone dos Santos; Reimão, Juliana Quero; Tempone, André G.; Sartorelli, Patrı́cia; Romoff, Paulete; Ferreira, Marcelo J. P.; Fávero, Oriana A.; Lago, João Henrique G.

doi:10.1007/s00436-010-1771-8

Cited by 56 publications

(37 citation statements)

References 21 publications

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“…Ghosh et al (2011 recently described the antileishmanial activity of roots of 'Indian Valerian' Valeriana wallichii (Ghosh et al 2011). A new antileishmanial flavanone has also been purified from the leaves of Baccharis retusa (Grecco et al 2010). Essential oil of Cymbopogon citratus exhibited in vitro antileishmanial activity on Leishmania amazonensis and its major component, citral, was identified as the main active principle (Santin et al 2009).…”

Section: Resultsmentioning

confidence: 94%

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

Sawadogo

Douaron²,

Maciuk³

et al. 2011

Parasitol Res

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After ethnobotanical surveys in central and western regions of Burkina Faso, five plants namely Lantana ukambensis (Verbenaceae), Xeoderris sthulmannii (Fabaceae), Parinari curatellifollia (Chrysobalanaceae), Ozoroa insignis (Anacardiaceae), and Ficus platyphylla (Moraceae) were selected for their traditional use in the treatment of parasitic diseases and cancer. Our previous studies have focused on the phytochemical, genotoxicity, antioxidant, and antiproliferative activities of these plants. In this study, the methanol extract of each plant was tested to reveal probable antileishmanial and antitrypanosomal activities. Colorimetric and spectrophotometric methods were used for the detection of antileishmanial and antitrypanosomal activities. Leishmania donovani (LV9 WT) and Trypanosoma brucei brucei GVR 35 were used to test the antileishmanial and antitrypanosomal activities, respectively. All extracts of tested plants showed a significant antitrypanosomal activity with minimum lethal concentrations between 1.5 and 25 μg/ml, the L. ukambensis extract being the most active. In the antileishmanial test, only the extract from L. ukambensis showed significant activity with an inhibitory concentration (IC(50)) of 6.9 μg/ml. The results of this study contribute to the promotion of traditional medicine products and are preliminary for the isolation of new natural molecules for the treatment of leishmaniasis and trypanosomiasis.

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Section: Resultsmentioning

confidence: 94%

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

Sawadogo

Douaron²,

Maciuk³

et al. 2011

Parasitol Res

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“…The viability of parasites was measured by the trypomastigote cellular conversion of MTT solution -bromide 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide) -in insoluble formazan by mitochondrial enzymes. 33 The extraction of formazan was performed with 10% (v/v) SDS for 18 h (100 mL/well) at 24 °C. 34 MTT and the test compound (without parasites) was added to an internal control to investigate possible oxidative reaction.…”

Section: Anti-trypanosomal Activitymentioning

confidence: 99%

Anti-trypanosomal activity of 1,2,3,4,6-penta-O-galloyl-β -D-glucose isolated from Plectranthus barbatus Andrews (Lamiaceae)

Santos¹,

Hiramoto²,

Lago³

et al. 2012

Quím. Nova

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Recebido em 25/5/12; aceito em 17/9/12; publicado na web em 9/11/12MeOH extract from the leaves of Plectranthus barbatus Andrews (Lamiaceae), showed in vitro anti-trypanosomal activity. The bioassay-guided fractionation resulted in the isolation of a gallic acid derivative, identified as 1,2,3,4,6-penta-O-galloyl-b-D-glucose (PGG), after thorough NMR and MS spectral analysis. Finally, this compound was tested against trypomastigote forms of T. cruzi and displayed an EC 50 value of 67 µM, at least 6.6-fold more effective than the standard drug benznidazole. This is the first occurrence of PGG in the Plectranthus genus and the first anti-parasitic activity described for PGG in the literature.

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“…Amastigotes were harvested from spleens of infected hamsters by differential centrifugation (Corrêa et al 2011). Trypanosoma cruzi trypomastigotes (Y strain) were maintained in LLC-MK2 (ATCC CCL 7) cells using RPMI-1640 medium supplemented with 2% FBS at 37 C in a 5% CO 2 -humidified incubator (Grecco et al 2010;Morais et al 2014). …”

Section: Parasite Maintenancementioning

confidence: 99%

“…NCTC (clone 929) cells were maintained in RPMI-1640 without phenol red and supplemented with 10% calf serum at 37 C under humidified atmosphere containing 5% CO 2 (Grecco et al 2010). …”

Section: Mammalian Cellsmentioning

confidence: 99%

Antiprotozoal activity of extracts and isolated triterpenoids of ‘carnauba’ (Copernicia prunifera) wax from Brazil

Almeida

Araújo

Carvalho

et al. 2016

Pharmaceutical Biology

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Context: 'Carnauba' wax is a natural product obtained from the processing of the powder exuded from Copernicia prunifera (Miller) H. E. Moore (Arecaceae). This material is widely used in the Brazilian folk medicine, including the treatment of rheumatism and syphilis. Objective: To investigate the antiprotozoal activity of hexane and EtOH extracts from the 'carnauba' wax as well as from the isolated compounds from the bioactive extracts. Material and methods: Two different samples of 'carnauba' (C. prunifera) waxes -types 1 and 4 -were individually extracted using hexane (EH) and EtOH (EE). Aliquots of hexane (type 1 -EH-1 and EH-4) and EtOH (type 4 -EE-1 and EE-4) extracts were tested against promastigote (2-200 lg/mL in DMSO during 48 h at 24 C) and amastigote (3-150 lg/mL in DMSO during 120 h at 37 C) forms of Leishmania infantum as well as against trypomastigote (3-150 lg/mL in DMSO during 24 h at 37 C) forms of Trypanosoma cruzi. Bioactive extracts EH-1 and EE-4 were subjected to a bioactivity-guided fractionation to afford three dammarane-type triterpenoids (1-3). The in vitro antiprotozoal activities of the obtained compounds were evaluated as described above. Additionally, the cytotoxicity activity of compounds 1-3 against mammalian conjunctive cells (NCTC -2-200 lg/mL in DMSO during 48 h at 37 C) was determined. Results: From the bioactive hexane and EtOH extracts from the 'carnauba' (C. prunifera) wax, were isolated three dammarane-type triterpenoids: (24R Ã )-methyldammar-25-ene-3b,20-diol (carnaubadiol, 1), (24R Ã )-methyldammara-20,25-dien-3-one (2) and (24R Ã )-methyldammara-20,25-dien-3a-ol (3). These compounds were identified based on the analysis of NMR and MS spectroscopic data. Compounds 1-3 were effective against the intracellular amastigotes of L. infantum, with IC 50 values ranging from 8 to 52 lM, while compounds 1 and 3 displayed activity against trypomastigote forms of T. cruzi with IC 50 values of 15 and 35 lM, respectively. The mammalian cytotoxicity assay demonstrated no damage to NCTC conjunctive cells up to 200 lM, except for compound 1, which demonstrated a CC 50 value of 34 lM. Conclusion: Based on the results, it was possible to conclude that the detected antiprotozoal bioactivity of 'carnauba' (C. prunifera) wax extracts could be related to the presence of the natural dammarane triterpenoid derivatives. The results suggested that these compounds could be used as promising scaffolds for drug design studies for leishmaniasis and Chagas disease. ARTICLE HISTORY

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Isolation of an antileishmanial and antitrypanosomal flavanone from the leaves of Baccharis retusa DC. (Asteraceae)

Cited by 56 publications

References 21 publications

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

Anti-trypanosomal activity of 1,2,3,4,6-penta-O-galloyl-β -D-glucose isolated from Plectranthus barbatus Andrews (Lamiaceae)

Antiprotozoal activity of extracts and isolated triterpenoids of ‘carnauba’ (Copernicia prunifera) wax from Brazil

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Isolation of an antileishmanial and antitrypanosomal flavanone from the leaves of Baccharis retusa DC. (Asteraceae)

Cited by 56 publications

References 21 publications

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

In vitro antileishmanial and antitrypanosomal activities of five medicinal plants from Burkina Faso

Anti-trypanosomal activity of 1,2,3,4,6-penta-O-galloyl-&#946; -D-glucose isolated from Plectranthus barbatus Andrews (Lamiaceae)

Antiprotozoal activity of extracts and isolated triterpenoids of ‘carnauba’ (Copernicia prunifera) wax from Brazil

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Anti-trypanosomal activity of 1,2,3,4,6-penta-O-galloyl-β -D-glucose isolated from Plectranthus barbatus Andrews (Lamiaceae)