Isolation of Acremonium species producing cephalosporine C (CPC) from forest soil in Gilan province, Iran

Abstract

Cephalosporin C (CPC) is a major precursor of cephalosporin antibiotics which is produced by a group of deuteromycets. After biosynthesis in optimized fermentation media, CPC is recovered and converted to a variety of potent cephalosporin antibiotic drugs. This study was performed to determine the mycoflora producing CPC in various parts of Iran soil. Soil samples were collected and cultured on selective media and Acremonium sp. were isolated. The presence of CPC antibiotic in the fermentation broths of these species was determined by a combination of biological, HPLC and mass spectrometry methods. From 350 fungi isolated, 21 (6%) were Acremonium spp, which one strain was able to produce CPC. This species was recognized as Acremonium persicinum according to its macroscopic and microscopic criteria. It is possible to apply different characteristics of the isolated species in strain optimization processes such as protoplast fusion.

Soil microbiota respond to green manure in organic vineyards

“…). Acremonium persicinum also hydrolyses cellulose and produces cephalosporin C, which is a major precursor of semisynthetic cephalosporin antibiotics used to treat a wide range of bacterial infections (Sarookhani and Moazzami ).…”

Influence of film mulching on soil microbial community in a rainfed region of northeastern China

“…Among fungi, the most dominant species were assigned to the genus Penicillium , which includes species that have been shown to play critical roles in the production of secondary metabolites or in the decomposition of organic matter 38 . Obvious differences in particular fungal taxa were also apparent across the soil samples from the three treatments.…”

Identification of Acremonium isolates from grapevines and evaluation of their antagonism towards Plasmopara viticola

“…The species A. persicinum has been reported as forest soil fungus in Iran (Sarookhani and Moazzami 2007), but never as endophytic fungus. The species A. sclerotigenum is known to be a common soil fungus, but its presence as endophyte has only been described in Quercus ilex (Collado et al 1999).…”