Isolation of a sulfur-oxidizing Streptomyces sp. from deteriorating bridge structures and its role in concrete deterioration

Vupputuri, Sravanthi; Fathepure, Babu Z.; Wilber, Gregory G.; Sudoi, Elias; Nasrazadani, S.; Ley, M. Tyler; Ramsey, Joshua D.

doi:10.1016/j.ibiod.2014.11.002

Cited by 22 publications

(7 citation statements)

References 32 publications

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“…A few members of the genus Alicyclobacillus have been described as sulfur- and ferrous-oxidizing [ 24 ]. A study by Vupputuri et al [ 25 ], analyzing the microbial diversity on concrete surfaces from deteriorated bridge structures, revealed that Alicyclobacillus spp. was the most dominant sulfur oxidizing acid producer that reduced the pH value of the culture medium from 6.7 to 2.8.…”

Section: Discussionmentioning

confidence: 99%

Characterization of sulfur oxidizing bacteria related to biogenic sulfuric acid corrosion in sludge digesters

et al. 2016

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BackgroundBiogenic sulfuric acid (BSA) corrosion damages sewerage and wastewater treatment facilities but is not well investigated in sludge digesters. Sulfur/sulfide oxidizing bacteria (SOB) oxidize sulfur compounds to sulfuric acid, inducing BSA corrosion. To obtain more information on BSA corrosion in sludge digesters, microbial communities from six different, BSA-damaged, digesters were analyzed using culture dependent methods and subsequent polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). BSA production was determined in laboratory scale systems with mixed and pure cultures, and in-situ with concrete specimens from the digester headspace and sludge zones.ResultsThe SOB Acidithiobacillus thiooxidans, Thiomonas intermedia, and Thiomonas perometabolis were cultivated and compared to PCR-DGGE results, revealing the presence of additional acidophilic and neutrophilic SOB. Sulfate concentrations of 10–87 mmol/L after 6–21 days of incubation (final pH 1.0–2.0) in mixed cultures, and up to 433 mmol/L after 42 days (final pH <1.0) in pure A. thiooxidans cultures showed huge sulfuric acid production potentials. Additionally, elevated sulfate concentrations in the corroded concrete of the digester headspace in contrast to the concrete of the sludge zone indicated biological sulfur/sulfide oxidation.ConclusionsThe presence of SOB and confirmation of their sulfuric acid production under laboratory conditions reveal that these organisms might contribute to BSA corrosion within sludge digesters. Elevated sulfate concentrations on the corroded concrete wall in the digester headspace (compared to the sludge zone) further indicate biological sulfur/sulfide oxidation in-situ. For the first time, SOB presence and activity is directly relatable to BSA corrosion in sludge digesters.

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Section: Discussionmentioning

confidence: 99%

Characterization of sulfur oxidizing bacteria related to biogenic sulfuric acid corrosion in sludge digesters

et al. 2016

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“…When these substances come into contact with the surface of cementitious material, they cause the formation of ettringite, which leads to the increase in internal pressure in the material and the subsequent formation of cracks. Further acidification leads to the accumulation of gypsum (CaSO 4 ) formed in different hydration states that significantly weaken the composite (Okabe et al 2007;Vupputuri et al 2015;Augustyniak et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Biofilms in the gravity sewer interfaces: making a friend from a foe

Augustyniak

Sikora

Grygorcewicz

et al. 2021

Rev Environ Sci Biotechnol

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Sewer systems are an integral part of our modern civilization and are an imperative underground infrastructure asset that our society relies on. In Western Europe alone, 92% of the resident pollution is connected to sewer systems. This extensive coverage of sewerage systems presents an ideal habitation for microorganisms to strive. Sewers can be considered continuous flow bioreactors. They are always colonized by bacteria, either in a planktonic state traveling along the pipe with the water flow or dragged in sediment, or organized as biofilms. Many studies have been devoted to the detrimental effects of microorganisms on sewer systems made of concrete. However, their metabolic activity can also be beneficial, lead to more effective wastewater treatment, or be beneficial for concrete pipes. This aspect has not been thoroughly studied to date and requires further investigation. Therefore, in this Review, we highlighted the positive and negative activity of biofilms and their participation in five proposed mass exchange points in gravity sewers. Furthermore, we systematized and reviewed state of the art regarding methods that could be potentially used to remove or engineer these biological structures to increase the sustainability of sewers and achieve a better pre-treatment of wastewater. We have also indicated research gaps that could be followed in future studies.

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“…These aggressive agents can easily deteriorate the structures, causing the reduction in material durability and performance [2,3]. This is thus a challenging problem mainly because of significant maintenance costs [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…At low pH, strong sulphur-oxidising bacteria such as Acidithiobacillus thiooxidans and Acidithiobacillus intermedius, start colonising the surface of concrete material. These bacteria are capable of further reducing the pH by oxidising thiosulphate and elemental sulphur to sulphuric acid [4]. Microbiological sulphate attack include a series of interactions occurring within the cement matrix as sulphates penetrate through it [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Calcium Extraction from Blast-Furnace-Slag-Based Mortars in Sulphate Bacterial Medium

2018

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Abstract:Wastewater structures, such as treatment plants or sewers can be easily affected by bio-corrosion influenced by microorganisms living in waste water. The activity of these microbes results in deterioration and can cause the reduction in structural performance of such structures. In order to improve the durability of mortar and concrete, different admixtures are being used and the best impact is observed in cement based materials combined with blast furnace slag. In this study, mortar samples with blast furnace slag were exposed to bacterial sulphate attack for 90 and 180 days. The leaching of calcium ions from the cement matrix and equivalent damaged depths of studied mortar samples were evaluated. The results showed more significant leaching of samples placed in bacterial environment, compared to the samples placed in non-bacterial environment. Similarly, the equivalent damaged depths of mortars were much higher for the bacteria-influenced samples. The slag-based cement mortars did not clearly show improved resistance in bacterial medium in terms of calcium leaching.

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Isolation of a sulfur-oxidizing Streptomyces sp. from deteriorating bridge structures and its role in concrete deterioration

Cited by 22 publications

References 32 publications

Characterization of sulfur oxidizing bacteria related to biogenic sulfuric acid corrosion in sludge digesters

Characterization of sulfur oxidizing bacteria related to biogenic sulfuric acid corrosion in sludge digesters

Biofilms in the gravity sewer interfaces: making a friend from a foe

Calcium Extraction from Blast-Furnace-Slag-Based Mortars in Sulphate Bacterial Medium

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