Isolation of a novel strain of Candida shehatae for ethanol production at elevated temperature

Tanimura, Ayumi; Nakamura, Toshihide; Watanabe, Ikumu; Ogawa, Jun; Shima, Jun

doi:10.1186/2193-1801-1-27

Cited by 40 publications

(26 citation statements)

References 36 publications

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“…Edgardo et al [19] used glucose for the selection of thermotolerant yeast strains and obtained a good potential thermotolerant yeast S. cerevisiae that was able to produce approximately 75% of the theoretical ethanol yield at 40˝C. In addition to using glucose as a substrate, other carbon sources, such as sugarcane juice [13], xylose [41], sugarcane blackstrap molasses [42], and inulin [43] have also been used for screening and selecting thermotolerant yeasts.…”

Section: Isolation and Selection Of Thermotolerant Yeast Strainsmentioning

confidence: 99%

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

et al. 2016

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Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of thermotolerant yeasts capable of growth and ethanol production at high temperatures were successfully isolated. Among these isolates, the newly isolated thermotolerant yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great potential for ethanol production from sweet sorghum juice (SSJ) at high temperatures. The maximum ethanol concentrations produced by this newly isolated thermotolerant yeast at 37˝C and 40˝C under the optimum cultural condition were 106.82 g¨L´1 and 85.01 g¨L´1, respectively, which are greater than values reported in the literatures. It should be noted from this study with SSJ at a sugar concentration of 250 g¨L´1 and an initial pH of 5.5 without nitrogen supplementation can be used directly as substrate for ethanol production at high temperatures by thermotolerant yeast S. cerevisiae DBKKU Y-53. Gene expression analysis using real-time RT-PCR clearly indicated that growth and ethanol fermentation activities of the thermotolerant yeast S. cerevisiae DBKKU Y-53 at a high temperature (40˝C) were not only restricted to the expression of genes involved in the heat-shock response, but also to those genes involved in ATP production, trehalose and glycogen metabolism, and protein degradation processes were also involved.

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Section: Isolation and Selection Of Thermotolerant Yeast Strainsmentioning

confidence: 99%

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

et al. 2016

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“…Jing-Ping Ge also reported the same yield of ethanol i.e., 0.31 g/g with corncob hydrolysate using Candida shehatae ACCC 20335 (Jing-Ping et al 2011). According to Tanimura, Candida shehatae strain ATY839 has produced 16.8 g/L i.e., 71.6 % of the maximum theoretical ethanol yield at 24 h (Ayumi et al 2012). …”

Section: Resultsmentioning

confidence: 99%

Enhanced bioethanol production from wheat straw hemicellulose by mutant strains of pentose fermenting organisms Pichia stipitis and Candida shehatae

et al. 2016

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The main aim of the present study was to mutate yeast strains, Pichia stipitis NCIM 3498 and Candida shehatae NCIM 3501 and assess the mutant’s ability to utilize, ferment wheat straw hemicellulose with enhanced ethanol yield. The organisms were subjected to random mutagenesis using physical (ultraviolet radiation) and chemical (ethidium bromide) mutagens. The mutant and wild strains were used to ferment the hemicellulosic hydrolysates of wheat straw obtained by 2 % dilute sulphuric acid and enzymatic hydrolysis by crude xylanase separately. Among all the mutant strains, PSUV9 and CSEB7 showed enhanced ethanol production (12.15 ± 0.57, 9.55 ± 0.47 g/L and yield 0.450 ± 0.009, 0.440 ± 0.001 g/g) as compared to the wild strains (8.28 ± 0.54, 7.92 ± 0.89 g/L and yield 0.380 ± 0.006 and 0.370 ± 0.002 g/g) in both the hydrolysates. The mutant strains were also checked for their consistency in ethanol production and found stable for 19 cycles in hemicellulosic hydrolysates of wheat straw. A novel element in the present study was introduction of chemical mutagenesis in wild type as well as UV induced mutants. This combination of treatments i.e., UV followed by chemical mutagenesis was practically successful.

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“…We also succeeded in isolating a yeast strain, ATY839, capable of ethanolic fermentation at temperatures above those previously reported for yeasts able to ferment both glucose and xylose (Tanimura et al 2012 ). Strain ATY839 produced a substantial amount of ethanol at up to 37 °C from 2 % glucose or 2 % xylose.…”

Section: High-temperature-tolerant Yeastsmentioning

confidence: 96%

“…To achieve high-effi ciency ethanol production, it is desirable to use both the glucose and xylose contained in the cellulose and hemicellulose (Kuhad et al 2011 ). However, a few types of yeast, such as Scheffersomyces stipitis (formerly known as Pichia stipitis ), Scheffersomyces shehatae (formerly known as Candida shehatae ), and Spathaspora passalidarum , have been found capable of xylose fermentation (Hou 2012 ;Jeffries et al 2007 ;Tanimura et al 2012 ); the simultaneous utilization of these sugars has been problematic. The yeast strain most generally used in current bioethanol production processes, S. cerevisiae , can ferment glucose derived from cellulose to ethanol; however, it normally lacks the ability to produce ethanol by fermenting the xylose present in hemicellulose (Jeffries and Jin 2004 ;Hasunuma and Kondo 2012 ;Kuhad et al 2011 ).…”

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confidence: 99%

Environmental Stresses to Which Yeast Cells Are Exposed During Bioethanol Production from Biomass

Shima

Nakamura

2015

Stress Biology of Yeasts and Fungi

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Bioethanol is one of the most important renewable fuels for the reduction of global warming effects and environmental damage caused by the worldwide utilization of fossil fuels. Yeasts such as Saccharomyces cerevisiae are frequently used for bioethanol production from mono-or disaccharides derived from biomass, including sugar cane, corn, and lignocellulosic materials. During bioethanol production, yeast cells are exposed to various environmental stresses including chemical, temperature, oxidative, and acid stresses. The development of yeast strains tolerant to such environmental stresses must improve the bioethanol production process. This chapter focuses on the environmental stresses to which yeast cells are exposed during bioethanol production. We also discuss the exploration and breeding of stress-tolerant yeast strains and their application to bioethanol production.

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Isolation of a novel strain of Candida shehatae for ethanol production at elevated temperature

Cited by 40 publications

References 36 publications

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Enhanced bioethanol production from wheat straw hemicellulose by mutant strains of pentose fermenting organisms Pichia stipitis and Candida shehatae

Environmental Stresses to Which Yeast Cells Are Exposed During Bioethanol Production from Biomass

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