Isolation of a novel siderophore from Pseudomonas cepacia

Abstract

Summary.A novel iron-binding compound was identified in ethyl acetate extracts of the supernates from Pseudornonas cepacia cultures. This compound, named azurechelin, was produced by 88% of P . cepacia strains isolated from the respiratory tract. Production of azurechelin was regulated by the iron concentration in the culture medium. Azurechelin enhanced the growth of P . cepacia in a medium containing transferrin 200 mg/L. Azurechelin released iron from transferrin in an equilibrium dialysis assay, suggesting that it could compete with transferrin for iron. Azurechelin could also stimulate iron uptake by P . cepacia. This siderophore appeared to have a novel structure with neither the typical characteristics of catechol nor of hydroxamate compounds.

The pobA gene of Burkholderia cenocepacia encodes a Group I Sfp-type phosphopantetheinyltransferase required for biosynthesis of the siderophores ornibactin and pyochelin

“…2(e). The absence of an orange zone around the growth of AHA27 on CAS plates confirms a previous observation that salicylate is not active as a siderophore in the CAS assay, although it has been proposed as a siderophore for B. cenocepacia (Sokol et al, 1992(Sokol et al, , 1999Visca et al, 1993).…”

Production of yellow-green fluorescent pigment by Pseudomonas fluorescens

“…This peak almost coincided with one of those peaks for pyochelin at 252 nm found in supernatant of P. aeruginosa (Meyer et al, 1992) or at 250 nm for P. cepacia (Sokol et al, 1992). The commercial salicylic acid solution (50 µg mL -1 ) spectrum gave two peaks, with maximum at 242 nm and 308 nm (Fig 1) and exhibited a completely different spectrum compared to those obtained with the G7 medium.…”

Purification and characterization of a novel chitinase from Burkholderia cepacia strain KH2 isolated from the bed log of Lentinus edodes, Shiitake mushroom.

“…The organism has been employed in biocontrol (Kang et al, 1998;Sfalanga et al, 1999) and bioremediation (Peres et al, 1999;Snyder et al, 2000). Various strains of B. cepacia have been reported as the source of a large variety of antifungal compounds, such as cepacin (Parker et al, 1984), pyrrolnitrin (Jayaswal et al, 1992), and siderophores (Sokol et al, 1992). Moreover, this strain can act as an effective biocontrol agent for Pythium-induced damping off, Aphanomyces-induced root rot of pea (Parke et al, 1991), and other fungal diseases (Fridlender et al, 1993).…”