Production of yellow-green fluorescent pigment by Pseudomonas fluorescens

Silva, Gildo Almeida da; Almeida, Erik Amazonas de

doi:10.1590/s1516-89132006000400009

Cited by 21 publications

(6 citation statements)

References 31 publications

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“…4) and this fluorescent peak is associated with the siderophore pyoverdine (Dartnell et al, 2013;Wasserman, 1965). Pyoverdine is an extracellular ironscavenging metabolite produced by P. aeruginosa and is associated with microbial virulence (da Silva and de Almeida, 2006). The fluorescence intensity of this high molecular weight OM within the P. aeruginosa growth curve, suggests that this Peak Cþ fluorescence could be derived from the building and exporting of pyoverdine.…”

Section: Pseudomonas Aeruginosamentioning

confidence: 99%

The in situ bacterial production of fluorescent organic matter; an investigation at a species level

et al. 2017

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a b s t r a c tAquatic dissolved organic matter (DOM) plays an essential role in biogeochemical cycling and transport of organic matter throughout the hydrological continuum. To characterise microbially-derived organic matter (OM) from common environmental microorganisms (Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa), excitation-emission matrix (EEM) fluorescence spectroscopy was employed. This work shows that bacterial organisms can produce fluorescent organic matter (FOM) in situ and, furthermore, that the production of FOM differs at a bacterial species level. This production can be attributed to structural biological compounds, specific functional proteins (e.g. pyoverdine production by P. aeruginosa), and/or metabolic by-products. Bacterial growth curve data demonstrates that the production of FOM is fundamentally related to microbial metabolism. For example, the majority of Peak T fluorescence (> 75%) is shown to be intracellular in origin, as a result of the building of proteins for growth and metabolism. This underpins the use of Peak T as a measure of microbial activity, as opposed to bacterial enumeration as has been previously suggested. This study shows that different bacterial species produce a range of FOM that has historically been attributed to high molecular weight allochthonous material or the degradation of terrestrial FOM. We provide definitive evidence that, in fact, it can be produced by microbes within a model system (autochthonous), providing new insights into the possible origin of allochthonous and autochthonous organic material present in aquatic systems.

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Section: Pseudomonas Aeruginosamentioning

confidence: 99%

The in situ bacterial production of fluorescent organic matter; an investigation at a species level

et al. 2017

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“…Kings B medium employs the fact that some species of Pseudomonas produces pyoverdine (a yellowish green fluorescent siderophores) in iron deficient environment [27]. Production of pyoverdine/siderophores endows Pseudomonas species with some level of virulence [14]. This may in turn make the bacterium resistant to some antibiotics, as shown in this study.…”

Section: Discussionmentioning

confidence: 72%

“…P. fluorescens and P. putida produce yellowish green pigments [8,10,11]. Pigment production by Pseudomonas species occur in the presence of antibiotics [12,13], in the presence of certain quantities of sulphate and magnesium salts [14], or at low iron concentrations [11]. These facts are made use of in the preparation of culture media for the isolation of Pseudomonas species.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Susceptibility of Resistant Pseudomonas Species Generated Through the Use of Differential and Selective Media for Isolation of Pseudomonas from Environmental Samples

Peekate

Woha

2018

SAJRM

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Water and soil samples were cultured on Cetrimide agar, Kings B agar, and nutrient agar supplemented with 50 μg.mL-1 chloramphenicol for the isolation of greenish pigment producing Pseudomonas species. Greenish pigment producing colonies that grew on the media were subjected to microscopic examination and selected physicochemical/biochemical tests to confirm that they belong to the Pseudomonas genus. Isolates confirmed to belong to the Pseudomonas genus were subjected to antibiotic susceptibility testing to various antibiotics using the disc diffusion method. The antibiotic sensitivity testing showed that two of the Pseudomonas isolates from nutrient agar plates supplemented with chloramphenicol showed resistance to Ampiclox, Rifampicin, and Norfloxacin. Also, one of the isolates showed resistance to Gentamycin, Amoxicillin, and Amoxicillin clavulanate. Two of the isolates from Kings B medium showed resistance to Ampiclox; another isolate showed resistance to Rifampicin, and one of the isolate that showed resistance to Ampiclox also showed resistance to Rifampicin, Chloramphenicol and Norfloxacin. Only one isolate from Cetrimide agar showed resistance to Ofloxacin and Nalidixic acid. Based on the EUCAST Breakpoint Table for P. aeruginosa, 12 out of the 14 Pseudomonas isolates (85.7%) were susceptible to Levofloxacin and Gentamycin, while 8 (57.1%) of the isolates were susceptible to Ciprofloxacin. The results indicate that Pseudomonas species isolated from the environment through the use of differential and selective media can become resistant to some antibiotics, and that Levofloxacin and Gentamycin could be used in the treatment of infections caused by antibiotic resistant Pseudomonas.

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“…The experimental measurements were analysed as a completely randomised design with four treatments and three repetitions. The yeasts were maintained in G7 medium (da Silva and de Almeida, 2006) and must agar (da Silva, 1996).…”

Section: Microorganismmentioning

confidence: 99%

Influence of Brettanomyces custersianus upon the activity of Saccharomyces cerevisiae strains during the tumultuous phase of vinification

Silva

Poletto

Poli

et al. 2011

Braz. arch. biol. technol.

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Production of yellow-green fluorescent pigment by Pseudomonas fluorescens

Abstract: ABSTRACT

Cited by 21 publications

References 31 publications

The in situ bacterial production of fluorescent organic matter; an investigation at a species level

The in situ bacterial production of fluorescent organic matter; an investigation at a species level

Antibiotic Susceptibility of Resistant Pseudomonas Species Generated Through the Use of Differential and Selective Media for Isolation of Pseudomonas from Environmental Samples

Influence of Brettanomyces custersianus upon the activity of Saccharomyces cerevisiae strains during the tumultuous phase of vinification

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