Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level

Abstract

This study isolated a novel erythritol-producing yeast strain, which is capable of growth at high osmolarity. Characteristics of the strain include asexual reproduction by multilateral budding, absence of extracellular starch-like compounds, and a negative Diazonium blue B color reaction. Phylogenetic analysis based on the 26S rDNA sequence and physiological analysis indicated that the strain belongs to the species Pseudozyma tsukubaensis and has been named P. tsukubaensis KN75. When P. tsukubaensis KN75 was cultured aerobically in a fed-batch culture with glucose as a carbon source, it produced 245 g/L of erythritol, corresponding to 2.86 g/L/h productivity and 61% yield, the highest erythritol yield ever reported by an erythritol-producing microorganism. Erythritol production was scaled up from a laboratory scale (7 L fermenter) to pilot (300 L) and plant (50,000 L) scales using the dissolved oxygen as a scale-up parameter. Erythritol production at the pilot and plant scales was similar to that at the laboratory scale, indicating that the production of erythritol by P. tsukubaensis KN75 holds commercial potential.

An Effective Method of Continuous Production of Erythritol from Glycerol by Yarrowia lipolytica MK1

“…Parameters of erythritol production by Yarrowia lipolytica MK1 in a chemostat culture in media with diff erent composition Table 2 It is worth noting that erythritol has been produced commercially from glucose (3). The available data obtained when using diff erent microorganisms on glucose media showed that the highest value of erythritol productivity in the fed-batch culture was 2.86 g/(L·h) with Pseudozyma tsukubaensis (8), whereas the highest value of erythritol production yield, 0.63 g/g, was noted by Lin et al (4) with Moniliella sp. N61188-12, when compared to the present results.…”

“…(6), Torula sp. (7) or Pseudozyma tsukubaensis (8). Moreover, erythritol may be produced at low pH values also from glycerol by the oleaginous yeast Yarrowia lipolytica (9,10).…”

Selective Hydrodeoxygenation of Cyclic Vicinal Diols to Cyclic Alcohols over Tungsten Oxide–Palladium Catalysts

“…In addition, 1,4-AHERY is easily produced by the dehydration of erythritol, [19] which is derived from glucose by fermentation. [20] The target product is 3-hydroxytetrahydrofuran (3-HTHF). 3-HTHF can be converted into 1,3-butanediol (1,3-BuD) by selective hydrogenolysis catalyzed by Ir-ReO x /SiO 2 (78 % selectivity), [18] and 1,3-BuD is used as an organic solvent for food flavoring agents and a raw material for polyurethane and polyester resins.…”

Synthesis of 2‐Butanol by Selective Hydrogenolysis of 1,4‐Anhydroerythritol over Molybdenum Oxide‐Modified Rhodium‐Supported Silica

“…[10] It was reported previously that the hydrogenolysis of erythritol over Ir-ReO x /SiO 2 gave 2-butanol as a byproduct, [14] however, the maximum yield of 2-butanol was 17 %. Erythritol, which can be produced easily by the fermentation of sugars, is regarded as a promising C 4 intermediate from biomass, [15][16][17][18][19][20] and the transformation of erythritol [21][22][23][24][25][26][27][28][29][30][31][32] and its derivatives, which include 1,4-anhydroerythritol (1,4-AHERY), to various C 4 chemicals such as butanediols, [14] tetrahydrofuran, [32,33] 2,5-dihydrofuran, [34] and 3-hydroxytetrahydrofuran (3-HTHF) [35] has been investigated. Therefore, the development of effective heterogeneous catalysts for the transformation of erythritol and its derivatives to 2-butanol is desirable.…”