Isolation of a novel Carica papaya α-amylase inhibitor with deleterious activity toward Callosobruchus maculatus

Farias, Luciana Ramalho de; Costa, Fábio T.; Souza, Lucas Anjos; Pelegrini, Patrícia B.; Grossi-de-Sá, Maria Fátima; Neto, Simone Maria; Bloch, Carlos; Laumann, R. A.; Noronha, Eliane Ferreira; Franco, Octávio L.

doi:10.1016/j.pestbp.2006.08.004

Cited by 46 publications

(24 citation statements)

References 46 publications

(54 reference statements)

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“…Various resources have been analysed in order to find a remedy for diabetes (ALI et al, 2006;BHANDARI et al, 2008;CHETHAN et al, 2008;LOIZZO et al, 2008;AGUILAR-SANTAMAR A et al, 2009;FRED-JAIYESIMI et al, 2009;SHOBANA et al, 2009). It has been reported that some of the compounds related to the defence mechanisms of plants may act as an inhibitors of hydrolytic enzymes such as alpha-amylase (FARIAS et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Alpha-amylase inhibition kinetics by caulerpenyne

2010

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Many algae have important secretions which are generally used for defensive purposes. These secretions have caught the attention of a number of researchers, as to whether or not these metabolites can be used in medical research. Among these metabolites, caulerpenyne (CYN) which is the main metabolite of the Caulerpa species, has had an important place in Caulerpa research since the beneficial effects related to its determined properties (such as cytotoxic, antiviral, antiproliferative and apoptotic) have been demonstrated in many scientific reports. In the present study, the inhibitory effect of CYN isolated from C. prolifera on alpha-amylase was investigated. The inhibition experiments were done with CYN by a spectrophotometric determination method. In order to evaluate the type of inhibition a Lineweaver-Burk plot was produced. The results obtained from enzyme kinetic studies exhibited a non-competitive type of inhibition, which is characterized by the difference of V max and K M from those of the free enzyme, of alpha-amylase in the presence of CYN. The present study showed that Caulerpa species may be a potential target for the production of diabetic drugs in the light of the results obtained for CYN.

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Section: Introductionmentioning

confidence: 99%

Alpha-amylase inhibition kinetics by caulerpenyne

2010

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“…In the light of accumulated published data, most authors hold the hypothesis of a defensive role of latices towards insects and phytopathogens. This belief is mainly supported by the phytochemical profile found in distinct latices (Konno et al, 2004;Taira, Ohdomari, Nakama, Shimoji, & Ishihara, 2005;Farias et al, 2007). It is not only of physiological importance but also of botanic interest to understand the metabolic activities of latices and their implications in plant protection.…”

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confidence: 95%

Essential Oils and Latices as Novel Antiviral Agent Against Potato Leaf Roll Virus and Analysis of Their Phytochemical Constituents Responsible for Antiviral Activity

Iftikhar¹,

Shahid²,

Javed³

et al. 2013

JAS

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The present work was aimed to limit the practice of using pesticides in the managing program of the potato leaf roll disease on potato plants as the misuse of some pesticides had caused severe problems. The study was focused to find compounds nontoxic and safe for the biome. The antiviral activity of essential oils and latices tapped from different plants was investigated against potato leaf roll virus (PLRV). Essential oils from Eucalyptus citriodora leaves, Clove buds, and Fennel seeds were extracted. Latex of Aloe vera, Calotropis procera and Ficus elastic were collected. Essential oil and latex spray solutions were prepared at 5 and 10% concentration. All the sprayings were done at 7 days interval. Inhibition of viral replication was monitored by amplification of PLRV capsid protein gene and mRNA expression using RT-PCR technique. Results depicted inhibitory potential of all the tested essential oils and latices against PLRV infestation and also the effect was found concentration dependent. Our data showed that Aloe vera latex and clove essential oil caused maximum inhibition viral mRNA at 10% concentration. Qualitatively analyzed Tannin, Phlobactanins, Saponin, Flavonoids, Sterols and Terpens from essential oils and latices gave positive results in most of the oils and lesser content in plant latices. In the GC-MS analysis, high contents of some bioactive phytochemical compounds identified were Eugenol in Clove buds, Eucalyptol, D-Limonene and L-Limonene both in Eucalyptus leaves and Fennel seed oils and Fenchone in Fennel seeds. As this research work illustrates, there is innumerable potential of plants essential oils/latex waiting to be evaluated and exploited against genetically and functionally diverse plant viruses.

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“…These particular structures have been defined as laticifers (Kekwick, 2001). Laticifer cells perform intense metabolic activity and in addition to the presence of typical Eukaryotic sub-cellular structures they synthesize and accumulate, under pressure, numerous distinct metabolites (Selvaraj et al, 1984;Han et al, 2000;Kalita and Saikia, 2004;Freitas et al, 2007;Farias et al, 2007). Studies performed aiming at understanding the biochemical profile of latex have offered convincing evidence for a defensive role played by these saps (Azarkan et al, 1997;Freitas et al, 2007;Ramos et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Latex fluids are endowed with insect repellent activity not specifically related to their proteins or volatile substances

Ramos

Araújo

Oliveira

et al. 2011

Braz. J. Plant Physiol.

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Latex is an endogenous milky fluid synthesized and accumulated under pressure in a network of laticifer cells. In this study, latices from five plant species were examined for deterrent effect on oviposition of two Coleoptera (Bruchidae) pests. The latex from Euphorbia tirucalli, Calotropis procera and Plumeria rubrae exhibited deterrent activity on oviposition of both Callosobruchus maculatusand Zabrotis subfasciatus beetles. The latex from Cryptostegia grandiflora and Himathantus drasticus were less effective to C. maculatus and Z. subfasciatus oviposition, respectively. Eggs laid on latex-treated seeds were not affected.The emergence and the mean time of development and weight of larvae grown in treated seeds were similar to the controls. The deterrent activity of C. procera and P. rubra was dose and time-dependent for Z. subfasciatus rather than to C. maculatus. The deterrent effect was completely eliminated if the whole latices were fractionated in protein fractions, rubber and small metabolites. Exposing insects to crude latices did not alter ovipostion on untreated seeds. These results suggest that latices possess deterrent activity on insect oviposition mediated by a repellent effect, but that proteins and volatile substances are probably not involved. The repellent-like activity can be considered as a defensive role played by these fluids that relies on the combined action of their components.

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Isolation of a novel Carica papaya α-amylase inhibitor with deleterious activity toward Callosobruchus maculatus

Cited by 46 publications

References 46 publications

Alpha-amylase inhibition kinetics by caulerpenyne

Alpha-amylase inhibition kinetics by caulerpenyne

Essential Oils and Latices as Novel Antiviral Agent Against Potato Leaf Roll Virus and Analysis of Their Phytochemical Constituents Responsible for Antiviral Activity

Latex fluids are endowed with insect repellent activity not specifically related to their proteins or volatile substances

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