Essential Oils and Latices as Novel Antiviral Agent Against Potato Leaf Roll Virus and Analysis of Their Phytochemical Constituents Responsible for Antiviral Activity

Iftikhar, Sehrish; Shahid, Ahmad Ali; Javed, Sundus; Nasir, Idrees Ahmad; Tabassum, Bushra; Haider, M. Saleem

doi:10.5539/jas.v5n7p167

Cited by 5 publications

(7 citation statements)

References 53 publications

(40 reference statements)

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“…Foliar treatment with clove oil nanoemulsion gave good results in reducing number of PVY infecting tomato plants. This result is in accordance with Iftikhar et al (2013) who mentioned that spraying potato plants with clove essential oil at conc. 5 and 10% significantly reduced Potato leaf roll virus infection in potato plants and also the effect was found to be concentration dependent.…”

Section: Discussionsupporting

confidence: 92%

“…Clove oil was applied against several microorganisms including plant viruses due to anti-viral activity. The possible anti-microbial action for clove oil is attributed to eugenol which consists about 68.65% of total clove oil content (Iftikhar et al, 2013). Spray application of 50 ppm aqueous solution of silver nano-particles on cluster bean leaves inoculated with Sun hemp rosette virus (SHRV) showed complete suppression of the disease (Jain and Kothari, 2014).…”

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confidence: 99%

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Antiviral Activity of Clove Oil Nanoemulsion Against Potato virus-Y (PVY)

Shafie

2017

Egyptian Journal of Phytopathology

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he inhibitory effect of clove essential oil nanoemulsion on Potato virus-Y (PVY) was studied in vitro and in vivo using two concentrations (5 and 10%). In vitro study, inoculum was mixed with the inhibitor and left to stand for 1, 2 and 3 days at (23±2°C) before plant inoculation. In vivo study, plants were sprayed with the inhibitor 1, 2 and 3 days either after or before inoculation. Chenopodium quinoa plants were used as a local lesion host, while Lycopersicon esculentum seedlings were used as a systemic host. In all cases clove oil nanoemulsion had a significant inhibitory effect on PVY infection either in vitro or in vivo trials. The highest inhibitory effect was achieved in vitro when the inoculum was mixed with the inhibitor at conc. of 10% for three days before inoculation, the local lesion development and the systemic infection were inhibited by 90% and 84.2%, respectively. In the case of in vivo experiments, the highest percentage of inhibition was detected when the plants were sprayed with the inhibitor at the same concentration three days before inoculation (81.6 and 80%, respectively). Moderate inhibitory effect was recorded either in vitro or in vivo, when the inoculum was neither mixed nor sprayed with the inhibitor for two days pre-virus inoculation. Whereas, the lowest inhibitory effect was detected when the inoculum was mixed with the inhibitor for one day before inoculation. ELISA test was used to confirm the results in all cases. Also all treatments gave a significant increase in photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids), total phenols and flavonoids compared with the infected control plants. RT-PCR technique was used to evaluate the inhibitory potential of the two concentrations of clove oil nanoemulsion tested against PVY infection. It was found that the effect of this antiviral agent was concentration dependent. Clove oil nanoemulsion showed strong virucidal activity as band intensity which was the lowest at conc. of 10% when it was mixed with the virus inoculum. The results of this work illustrate, clove essential oil nanoemulsion seems to be a promising tool as an antiviral agent against Potato virus-Y on tomato plants.

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

Antiviral Activity of Clove Oil Nanoemulsion Against Potato virus-Y (PVY)

Shafie

2017

Egyptian Journal of Phytopathology

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“…Foliar treatment with essential oil of clove and fennel showed high activity in reducing the number of PVY-infected pepper plants. Similar results were noticed by Iftikhar et al (2013), who mentioned that foliar treatment with clove and fennel essential oil significantly reduced Potato leaf roll virus infection in potato plants. Lippia nodiflora extracts were highly effective in inducing systemic resistance in potato plants against PVY infection (Al-Ani et al, 2011).…”

Section: Discussionsupporting

confidence: 87%

“…Spraying the foliage by the extracts exhibited a protection period to the plants against PVY infection up to one month in the presence of virus source with the vector Myzus persicae (Al-Ani et al, 2011). Essential oil of clove caused maximum inhibition of Potato leaf roll virus replication (Iftikhar et al, 2013). The essential oil of Artemisia and lemongrass and ginger resulted in more than 50% inhibition of TMV on tobacco plants (Lu et al, 2013).…”

mentioning

confidence: 98%

Induction of Resistance in Pepper Plants Against Potato Virus Y (PVY)NTN by Two Medicinal and Aromatic Plant Essential Oils and Their Major Components

Shafie¹,

Kheder²,

Farghaly³

2017

Egyptian Journal of Phytopathology

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he effects of essential oils of clove and fennel seeds and their major components as foliar spray were screened for inducing resistance against Potato Virus Y in vivo in both systemic and local lesion hosts at different concentrations. Clove oil at conc. of 15% gave the highest inhibitory effect against PVY infection than fennel oil. The inhibition percentages were 85, 80 % in pepper as a systemic host and up to 90, 87 %, respectively in Chenopodium amaranticolor as a local lesion host when applied 48 hrs before virus inoculation. Whereas, the inhibitory effect was less pronounced when essential oils were sprayed on the tested plants 24 hrs before virus inoculation. However, the severity of symptoms was assessed by visual inspection. ELISA test was used to confirm the results in all cases. When the major components of essential oils were applied individually, Eugenol gave the highest inhibitory effect (90%) on PVY-systemically infected pepper plants and 91.8% on Ch. amaranticolor as local infected plants when applied 48 hrs before virus inoculation followed by Anethol. While, Limonene was less effective in reduce PVY infection. Considerable increase in total protein content in pepper plants sprayed with essential oils and their major components before virus inoculation was recorded compared with healthy and infected control plants. Moreover, SDS-PAGE revealed a new protein band 20 KDa which was found only in pepper plants sprayed with clove oil at conc. of 15%. Also new proteins 19, 20 KDa and 25 KDa were found only in pepper plants sprayed with Eugenol. It has been suggested that, the induced proteins may help to limit virus spread or multiplication.

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“…Antiviral effects of plant extracts and plantderived compounds against a number of plant viruses have been reported (Iftikhar et al, 2013;Milad et al, 2014;Rasoulpour et al, 2017). Here, we first demonstrated the antiviral effects of LCE against TMV.…”

Section: Discussionmentioning

confidence: 83%

Investigation of Antiviral Potential of Licorice (Glycyrrhiza Glabra L.) Crude Extract Against Tobacco Mosaic Virus

2020

THE JAPS

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Tobacco mosaic virus (TMV) is a devastating plant RNA virus infecting a wide range of plants causing considerable losses to agriculture products. Various dissemination means of TMV makes it particularly difficult to control. Application of plant-derived antiviral compounds has gained attention as one of the new control measures against viral diseases. This study was conducted to examine the antiviral effects of licorice (Glycyrrhiza glabra L.) crude extract (LCE) against TMV. To this end, TMV particles were purified and treated with different LCE concentrations (0.5, 1.0, 2.5 and 5.0%). Two hosts of TMV (Nicotiana glutinosa L. and N. tabacum L.) were mechanically inoculated with the LCE-treated TMV preparations and antiviral effects were assessed by measuring different disease parameters in the plants including infection rate, number of local lesion, symptom index, and virus accumulation. These parameters were significantly reduced when LCE was used to treat the TMV preparation or it was applied 24 h before TMV inoculation. An inverse relationship was found between LCE concentrations and various studied parameters. Application of LCE 24 h after TMV inoculation had no significant effect on disease parameters suggesting that LCE directly affects TMV rather than activating plant defence system. The results showed that licorice can be a promising source of novel antiviral compounds againts TMV.

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Essential Oils and Latices as Novel Antiviral Agent Against Potato Leaf Roll Virus and Analysis of Their Phytochemical Constituents Responsible for Antiviral Activity

Cited by 5 publications

References 53 publications

Antiviral Activity of Clove Oil Nanoemulsion Against Potato virus-Y (PVY)

Antiviral Activity of Clove Oil Nanoemulsion Against Potato virus-Y (PVY)

Induction of Resistance in Pepper Plants Against Potato Virus Y (PVY)NTN by Two Medicinal and Aromatic Plant Essential Oils and Their Major Components

Investigation of Antiviral Potential of Licorice (Glycyrrhiza Glabra L.) Crude Extract Against Tobacco Mosaic Virus

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