Isolation of a New Catechin, Tea Catechin II or Gallo-Catechin from Green Tea

Tsujimura, Michiyo

doi:10.1271/bbb1924.10.140

Cited by 4 publications

(2 citation statements)

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“…These catechins have been discovered long before. EC, ECG, and EGC were discovered in the early 20th century [ 13 , 14 , 15 ], and EGCG was first reported by Bradfield et al in 1948 [ 16 ]. These four major catechins have been extensively investigated for decades.…”

Section: Phenolics In Green Teasmentioning

confidence: 99%

Advances in Nanodelivery of Green Tea Catechins to Enhance the Anticancer Activity

Jiang

2021

Molecules

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Cancer is one of the leading causes of death globally. A variety of phenolic compounds display preventative and therapeutic effects against cancers. Green teas are rich in phenolics. Catechins are the most dominant phenolic component in green teas. Studies have shown that catechins have anticancer activity in various cancer models. The anticancer activity of catechins, however, may be compromised due to their low oral bioavailability. Nanodelivery emerges as a promising way to improve the oral bioavailability and anticancer activity of catechins. Research in this area has been actively conducted in recent decades. This review provides the molecular mechanisms of the anticancer effects of catechins, the factors that limit the oral bioavailability of catechins, and the latest advances of delivering catechins using nanodelivery systems through different routes to enhance their anticancer activity.

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Section: Phenolics In Green Teasmentioning

confidence: 99%

Advances in Nanodelivery of Green Tea Catechins to Enhance the Anticancer Activity

Jiang

2021

Molecules

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“…Oxidative stress causes several diseases (Ishizawa et al 2014;Tajima et al 2012;Yamano et al 2015). Catechin polyphenols found in green tea (Tsujimura 1930(Tsujimura , 1934 have health benefits due to antioxidant activity (Higdon and Frei 2003;Williamson and Manach 2005). Among them, (-)-epigallocatechin-3gallate (EGCG) is the major catechin in green tea, and it has been suggested to be responsible for the majority of the potential health benefits attributed to green tea consumption (Nagle et al 2006).…”

Section: Introductionmentioning

confidence: 99%

EGCG improves recombinant protein productivity in Chinese hamster ovary cell cultures via cell proliferation control

Yamano

Ômasa

2018

Cytotechnology

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Chinese hamster ovary cell lines are good manufacturing practice-certified host cells and are widely used in the field of biotechnology to produce therapeutic antibodies. Recombinant protein productivity in cells is strongly associated with cell growth. To control cell proliferation, many approaches have previously been tested including: genetic engineering, chemical additives such as cell cycle inhibitors, and temperature shift of the culture. To be widely adopted in the biopharmaceutical industry, the culture methods should be simple, uniform and safe. To this end, we examined the use a natural compound to improve the production capacity. In this study, we focused on the antioxidants, catechin polyphenols, which are found in green tea, for cell proliferation control strategies. (-)-Epigallocatechin-3-gallate (EGCG), the major catechin that induces G0/G1 cell cycle arrest, was investigated for its effect on recombinant protein production. Adding EGCG to the cell culture media resulted in slower cellular growth and longer cell longevity, which improved the specific productivity and total yield of recombinant IgG1 in batch cultures by almost 50% for an extra 2 or 3 days of culture. A lower L-glutamine consumption rate was observed in cells cultured in EGCG-containing media, which may be suggesting that there was less stress in the culture environment. Additionally, EGCG did not affect the N-glycan quality of IgG1. Our results indicated that adding EGCG only on the first day of the culture enhanced the specific productivity and total amount of recombinant protein production in batch cultures. This approach may prove to be useful for biopharmaceutical production.

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Catechins prevent monoclonal antibody fragmentation during production via fed‐batch culture of Chinese hamster ovary cells

Yamaguchi,

Ishikawa,

Fukuda

et al. 2024

Biotechnology Progress

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Chinese hamster ovary (CHO) cells are widely used for the industrial production of therapeutic monoclonal antibodies (mAbs). To meet the increasing market demands, high productivity, and quality are required in cell culture. One of the critical attributes of mAbs, from a safety perspective, is mAb fragmentation. However, methods for preventing mAbs fragmentation in CHO cell culture are limited. In this study, we observed that the antibody fragment content increased with increasing titers in fed‐batch cultures for all three cell lines expressing recombinant antibodies. Adding copper sulfate to the culture medium further increased the fragment content, suggesting the involvement of reactive oxygen species (ROS) in the fragmentation process. Though antioxidants may be helpful to scavenge ROS, several antioxidants are reported to decrease the productivity of CHO cells. Among the antioxidants examined, we observed that the addition of catechin or (−)‐epigallocatechin gallate to the culture medium prevented fragmentation content by about 20% and increased viable cell density and titer by 30% and 10%, respectively. Thus, the addition of catechins or compounds of equivalent function would be beneficial for manufacturing therapeutic mAbs with a balance between high titers and good quality.

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Isolation of a New Catechin, Tea Catechin II or Gallo-Catechin from Green Tea

Abstract: Nos. 7-9]145 pure specimen no depression of melting point was observed.Analysis:-12. Absorption Spectra.

Cited by 4 publications

References 0 publications

Advances in Nanodelivery of Green Tea Catechins to Enhance the Anticancer Activity

Advances in Nanodelivery of Green Tea Catechins to Enhance the Anticancer Activity

EGCG improves recombinant protein productivity in Chinese hamster ovary cell cultures via cell proliferation control

Catechins prevent monoclonal antibody fragmentation during production via fed‐batch culture of Chinese hamster ovary cells

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