Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds

Sá, Daniele Maria Alves Teixeira; Reicher, Fany; Braga, Renata Chastinet; Beltramini, Leila Maria; Moreira, Renato de Azevedo

doi:10.1016/j.phytochem.2009.08.011

Cited by 15 publications

(11 citation statements)

References 38 publications

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“…Other glycoproteins and simple-sugar carbohydrates tested could not inhibit the MPLEC activity even at the maximum concentration used (2500 µg/ml for glycoproteins and 250 nM for simple sugars). The results obtained differ from the observed on M. sloanei and M. derringiana seeds' lectins, both presenting specificity to D-galactose and its derivations (Mo and Goldstein, 1994;Teixeira-Sá et al, 2009). λ-Carrageenan is a sulfated polysaccharide extracted from sea algae, normally constituted by esters of potassium, sodium, calcium, magnesium and ammonium sulfate and repeated units of β-D-galactose-2-sulfate-(1→4)-α-D-galactose-2,6-disulfate.…”

Section: Hemagglutinating Activity and Carbohydrate Inhibitioncontrasting

confidence: 63%

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

Lacerda¹,

Moreira²,

Nascimento³

et al. 2015

J. Med. Plants Res.

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Lectins are proteins present in all living beings capable of interacting specifically and reversibly to carbohydrates or glycoconjugates form. They stand out in this family of lectins legumes, which are of special scientific interest because they have different biological functions. This study investigated the presence of a lectin in Brazilian seeds of Mucuna, proceeding with their isolation and evaluation of its antinociceptive effects, anti-inflammatory and antihemolytic activity. Saline extracts of Mucuna pruriens were investigated for the presence of hemagglutinating activity by serial dilution and applied to Sephacryl S-200HR columns and DEAE Sephacel HiPrepFF to purify their lectin. After assessing the purity and molecular weight by polyacrylamide agarose gel electrophoresis (PAGE), the hemolytic activity on human erythrocytes was determined, as well as its antinociceptive action by models of writhing induced by acetic acid and formalin, in addition to its anti-inflammatory activity by testing the paw edema induced by carrageenan. The results indicated that Brazilian Mucuna seeds had a lectin that was purified from seeds having molecular weight of 60.0 kDa and only able to agglutinate erythrocytes of rabbit. The lectin showed an antihemolytic effect on human erythrocytes by not causing hemolysis in these cells compared to the negative control. The administration of lectin in rodents intraperitoneally inhibited the writhing by 99% (2.5 mg/kg), induced by acetic acid, as well as in the method where the formalin nociceptive stimulus was reduced by 51% in stage one and 77% in stage two of the test, using 2.5 mg/kg lectin. The anti-inflammatory activity demonstrated a decrease in paw edema induced by carrageenan in 72% better result than indomethacin control where there was a reduction of only 48% of edema. The study supports the presence of a lectin in Brazilian seeds of M. pruriens with antinociceptive, anti-inflammatory and antihemolytic activities.

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Section: Hemagglutinating Activity and Carbohydrate Inhibitioncontrasting

confidence: 63%

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

Lacerda¹,

Moreira²,

Nascimento³

et al. 2015

J. Med. Plants Res.

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“…Reticulação: A reticulação seguiu a metodologia de Teixeira-Sá et al (2009), onde usouse 1 g de mucilagem precipitada, 0,3 mL de epicloridrina e 3 mL de solução de hidróxido de sódio 3 M. A mistura foi deixada em estufa por 24 horas a 40 °C, e por 12 horas a 70 °C. O reticulado foi lavado com água destilada até atingir pH neutro e armazenado em solução de NaCl 0,15 N, ácido ascórbico e azida sódica, sob refrigeração até o momento do uso.…”

Section: Métodosunclassified

“…Como podemos verificar houve ótima afinidade entre a mucilagem reticulada e a proteína, pois obtiveram um rendimento na purificação de 100% (5,2 mg.g -1 ). Teixeira-Sá et al (2009), purificaram a lectina por cromatografia de afinidade, onde reticularam com epicloridrina os polissacarídeos extraídos de Mucuna sloanei e utilizaram como adsorvente, com isso obtiveram um rendimento na purificação da lectina de 5.4 mg.g -1 . Biopharm., v. 57, p. 19-34, 2004.…”

Section: Capacidade De Adsorçãounclassified

Extração, Precipitação E Reticulação Da Mucilagem De Jaracatiá (Carica Quercifolia (A. St.-Hil) Hieron) Para Uso Como Adsorvente De Proteínas

Heidemann¹,

Faccio²,

Quadri³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -A mucilagem é composta quimicamente por água, pectinas, açúcares e ácidos orgânicos, tornando-se viscosa na presença de água. O objetivo deste estudo foi a reticulação da mucilagem de jaracatiá (Carica quercifolia (A. St.-Hil.) Hieron) precipitada, a qual é composta por polissacarídeos naturais, para uso como adsorvente de proteínas, aproveitando assim os produtos da biodiversidade brasileira. Após a extração e precipitação com etanol, a mucilagem foi reticulada com epicloridrina. O rendimento da mucilagem de jaracatiá precipitada e da reticulação, os grupos funcionais antes e após a reticulação e a capacidade de adsorção do reticulado utilizando papaína foram avaliados. O rendimento da mucilagem precipitada em relação a massa de polpa do fruto foi de 1-1,5% e, após a reticulação o rendimento ficou em torno de 20% de mucilagem reticulada em relação à massa inicial de mucilagem precipitada. Os espectros FTIR mostraram que houveram mudanças nas bandas características com a reticulação. A capacidade da adsorção de papaína mostrou-se satisfatória, em torno de 50%.

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“…Further, naturally occurring cross-linked polysaccharides, which are inexpensive and simple to prepare with epichlorhydrin or divinyl sulphone, have also been extensively used as adsorbent matrices for isolating galactose-specific lectins (Lis & Sharon, 1981). These include guar gum or guaran (Sigma-Aldrich), a galactomannan polysaccharide that binds lectins with anomeric preference for Ǐ-galactose (Alencar et al, 2010;Santos et al, 2009;Souza et al, 2011;Sultan et al, 2009), a polysaccharide isolated from Spondias purpurea (Teixeira et al, 2007), a crosslinked seed gum matrix prepared from plant Leucaena leucocephala (Seshagirirao et al, 2005), a galactomannan from Adenanthera pavonina (Moreira et al, 1998;Teixeira-Sá et al, 2009;Trindade et al, 2006), and a cross-linked galactoxyloglucan from Mucuna sloanei (Teixeira-Sá et al, 2009). Moreover, adsorbent matrices from yeast glucan or curdlan, a polymer of glucose, were prepared to isolate glucose-specific lectins (Mikes & Man, 2003).…”

Section: Adsorbent Matricesmentioning

confidence: 99%