Isolation of a 14 kDa antigen from Taenia solium cyst fluid by HPLC and its evaluation in enzyme linked immunosorbent assay for diagnosis of porcine cysticercosis

Assana, Emmanuel; Kanobana, Kirezi; Tume, Christopher; Zoli, P. A.; Nguekam,; Geerts, S.; Berkvens, Dirk; Dorny, Pierre

doi:10.1016/j.rvsc.2006.09.006

Cited by 12 publications

(10 citation statements)

References 31 publications

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“…The ELISA for the detection of antibodies against T. solium cysticerci (Ab-ELISA) was based on a 14 kDa antigen (F3 antigen) purified from T. solium cyst fluid by a two-step chromatography, as described by Assana et al (2007). The antigen (F3) was diluted at 1 μg/ml in a carbonate buffer (0.06 m , pH 9.6) for coating (1 h at 37°C and overnight at 4°C).…”

Section: Methodsmentioning

confidence: 99%

Pig-farming systems and porcine cysticercosis in the north of Cameroon

et al. 2010

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A survey was conducted in 150 households owning 1756 pigs in the rural areas of Mayo-Danay division in the north of Cameroon. A questionnaire survey was carried out to collect information on the pig-farming system and to identify potential risk factors for Taenia solium cysticercosis infection in pigs. Blood samples were collected from 398 pigs with the aim of estimating the seroprevalence of T. solium cysticercosis. The results showed that 90.7% of the pigs are free roaming during the dry season and that 42.7% of households keeping pigs in the rural areas have no latrine facility. Seventy-six per cent of the interviewed pig owners confirmed that members of the household used open-field defecation. Enzyme-linked immunosorbent assay (ELISA) for antigen and antibody detection showed an apparent prevalence of cysticercosis of 24.6% and 32.2%, respectively. A Bayesian approach, using the conditional dependence between the two diagnostic tests, indicated that the true seroprevalence of cysticercosis in Mayo-Danay was 26.6%. Binary logistic regression analysis indicated that a lack of knowledge of the taeniasis–cysticercosis complex and the absence of a pig pen in the household were associated with pig cysticercosis.

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Section: Methodsmentioning

confidence: 99%

Pig-farming systems and porcine cysticercosis in the north of Cameroon

et al. 2010

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“…Studies have demonstrated the high speciϐicity and sensitivity of the native 14 kDa protein isolated from T. solium cysticerci using the ELISA , Greene et al 2000, Obregon-Henao et al 2001, Assana et al 2007.…”

Section: Detection Of Circulating Antigen Using Ag-elisamentioning

confidence: 99%

“…However, a number of studies have reported poor antigenicity for antigens lacking glycosylation (Obregon-Henao et al 2001). Among the native antigens analyzed, the 14 kDa protein isolated from T. solium cysticerci is considered one of the most promising proteins (Tsang et al 1991, Greene et al 2000, Obregon-Henao et al 2001, Assana et al 2007.…”

Section: Introductionmentioning

confidence: 99%

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection.

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“…The exact origin of these possible cross-reactions, however, remains a question. Assana et al (2007) for instance showed that GP14 does not cross-react with serum from pigs infected with common porcine parasites such as T. hydatigena, T. asiatica and Trichinella spiralis. The fact that none of the two T. hydatigena positive pigs in our study tested positive in EITB also shows that the EITB does not cross-react with this parasite, at least.…”

Section: Eitb Resultsmentioning

confidence: 99%