Isolation, identifications and antimicrobial susceptibility pattern of coagulase positive &lt;i&gt;Staphylococcus&lt;/i&gt; from subclinical mastitic dairy cattle in and around Haramaya University

Tafa, Firaol; Terefe, Yitagele; Tamerat, Nateneal; Zewdu, Endrias

doi:10.4314/evj.v19i2.8

Cited by 13 publications

(6 citation statements)

References 13 publications

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“…The present study has demonstrated the presence of high level of multidrug resistance of isolated S. aureus, 98.39% (61/62), especially among commonly used drugs like penicillin and tetracycline. The current study is consistent with Tafa et al [ 85 ], Taddesse et al [ 86 ] and Abera et al [ 57 ] who reported 87.6%, 90% and 94.4% of S. aureus resistance to multiple drugs, respectively. But the results of this study were higher than the findings reported in Brazil [ 87 ] and Ethiopia [ 88 ] where 64.4% and 45.1% of S. aureus isolates, respectively, resisted three or more antibiotics.…”

Section: Discussionsupporting

confidence: 93%

Isolation, Identification, and Susceptibility Profile of E. coli, Salmonella, and S. aureus in Dairy Farm and Their Public Health Implication in Central Ethiopia

Geletu¹,

Usmael

Ibrahim

2022

Veterinary Medicine International

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A cross-sectional study was conducted from November 2018 to May 2019 in Bishoftu and Dukem in central part of Ethiopia. The objectives of the present study were to isolate and identify S. aureus, E. coli, and Salmonella from dairy cattle, personnel, and equipment at farms. In addition to this, antimicrobial resistance profiles of the isolates were determined. A total of 607 samples consisting of fresh cow milk (125), fecal sample (211), nasal swab (211), pooled milkers’ hand swabs (20), pooled floor swabs (20), and tank milk (20) samples were collected from 20 dairy farms, which included 211 animals. Structured questionnaire was designed and administered to dairy farm owners and dairy food consumers to assess their consumption behavior and antibiotics usage. The samples were examined for the presence of S. aureus, E. coli, and Salmonella following standard techniques and procedures outlined by the International Organization for Standardization. Subsequently, 62 (15.7%) of S. aureus were isolated from 396 of the totals analyzed samples for S. aureus. Out of the 62 isolated S. aureus, 35/211(16.7%), 19/125(15.2%), 6/20(30%), 2/20(10%), and 0/20(0%) were from nasal swabs, udder milk, bulk milk, pooled hand swab, and floor swabs, respectively. On the other hand, 30 (7.6%) of E. coli were isolated from 396 of the totals analyzed samples for E. coli. Out of the 30 isolated E. coli, 17/211(8.1%), 12/125(9.6%), 0/20(0%), 0/20(0%), and 1/20(5%) were from faeces, udder milk, bulk milk, pooled hand swab, and floor swabs, respectively. In line with this, 13 (4.8%) of Salmonella were isolated from 271 of the totals analyzed samples for Salmonella. Out of the 13 isolated Salmonella, 10/211(4.7%), 2/20(10%), 0/20(0%), and 1/20(5%) were from faeces, bulk milk, pooled hand swab, and floor swabs, respectively. Subsequently, 62 of S. aureus, 30 of E. coli, and 13 of Salmonella isolates were subjected to antimicrobial susceptibility testing, and all isolates were resistant to at least one or more antimicrobials tested. Penicillin, methicillin, and trimethoprim/sulfamethoxazole are drugs to which a large proportion of isolated S. aureus were highly resistant, which range from 90% to 100%. From 30 tested E. coli, they showed (83%) resistance to Tetracycline and 80% to Vancomycin. The resistance level of 13 isolated Salmonella was 69% to Nalidixic acid and 54% to Vancomycin. Multiple drug resistance was detected in high (98.4%) for S. aureus, (56.7%) for E. coli, and (53.9%) for Salmonella. High proportion of multiple drug resistant in the dairy farm alerts concern for animal and public health as these drugs are used widely for treatment and prophylaxis in animals and humans.

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Section: Discussionsupporting

confidence: 93%

Isolation, Identification, and Susceptibility Profile of E. coli, Salmonella, and S. aureus in Dairy Farm and Their Public Health Implication in Central Ethiopia

Geletu¹,

Usmael

Ibrahim

2022

Veterinary Medicine International

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“…Generally, in this study, possible knowledge gaps were identified in dairy farmers regarding the occurrence of mastitis and its possible risk factors. From the respondents, about 21.2% wash their hands between consecutive milking of cows and around 75.8% did not use a towel to clean the udder, which agrees with several findings [ 14 , 18 , 19 ]. When milk is consumed unpasteurized, as it often occurs in developing countries where regulation and oversight of the dairy industry are lacking, dairy can serve as a vector for zoonotic transmission of disease and can contain adulterants such as antibiotic residues [ 5 ].…”

Section: Discussionsupporting

confidence: 89%

Prevalence and Risk Factors of Bovine Mastitis in Southern Zone of Tigray, Northern Ethiopia

Baraki

Teklue

Atsbha³

et al. 2021

Veterinary Medicine International

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Mastitis is the most common disease of adult dairy cattle. The disease continues to be one of the most perplexing and costly diseases of dairy cattle. The present study was conducted to detect bovine mastitis cows and identify potential risk factors for the distribution of bovine mastitis of smallholder dairy farmers using the California mastitis test. A cross-sectional study was conducted from September 2013 to May 2015 in the southern zone of Tigray, Northern Ethiopia, on 146 head of randomly selected cattle, of which 99 (67.8%) were crossed zebu and 47 (32.2%) pure local zebu using the California mastitis test and questionnaire. The overall prevalence was 65 (45.5%), of which 72.3% of crossed local and 27.69% of local zebu were found positive for the CMT test. The investigated risk factors were the season of lactation (OR = 0.510, CI = 0.208–1.247, P = 0.140 ), washing hands between consecutive milking events (OR = 0.354, CI = 0.146–0.856, P = 0.021 ), and history of previous mastitis (OR = 0.441, CI = 0.226–0.858, P = 0.016 ), which had significant ( P < 0.05 ) effect on the present prevalence of bovine mastitis in the study area. A high prevalence of bovine mastitis was observed in the southern zone of Tigray. The summer season of lactation and not washing hands between consecutive milking of cows were significant mastitis causation risk factors in addition to the history of previous mastitis disease history.

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“…The finding of 9.9% prevalence of clinical mastitis and 31.8% prevalence of subclinical mastitis in the present study is in accordance with the view of scholar's that subclinical mastitis is 3 to 4 times more frequent than clinical mastitis (Radostits et al, 2007). The study closely agrees with the reports of Demelash Biffa et al (2005), Birhanu Mekibib et al (2010), Mesele Abera et al (2013) and Rigbe Haftu et al (2012), however it was lower than the report of Firaol Tafa et al (2015) and Ararsa Duguma et al (2014). The higher prevalence of subclinical mastitis might be the strong cows' udder defense mechanism (Jha et al, 2010), whereas the lower prevalence of clinical mastitis is attributed to treatment after appearance of the clinical signs.…”

Section: Discussionsupporting

confidence: 90%

Prevalence and risk factors of bovine mastitis in Ambo town of West Shewa Zone, Oromia, Ethiopia

Kebebew¹,

Sarba²

2016

Ethiop. Vet. J.

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A cross-sectional study was conducted to estimate the prevalence and associated risk factors of mastitis in dairy cows from November, 2012 to July, 2013 in Ambo town of West Shewa Zone, Oromia Regional State. Thorough clinical examination was made on all lactating cows for evidence of signs of clinical mastitis followed by collection of milk sample for examination of gross changes of milk secretion and screening using California Mastitis Test (CMT). Mastitis was categorized as clinical if lactating cows exhibited clinical features of mastitis, or subclinical based on degree of coagulation up on examination using CMT. A total of 151 dairy cows were selected from all volunteer dairy farms in Ambo town of West Shewa Zone of Oromia region state. Sixty three (41.7%) cows had mastitis, of which 9.9% (15/151) were clinical and 31.8% (48/151) were subclinical mastitis cases. The quarter level prevalence was 44.4% (268/604), comprising 9.3% (56/604) clinical and 31.8% (198/604) subclinical forms of mastitis. In addition, 5.5% (33/604) of teats were found to be blind up on clinical examination of udder and teat. The Chi-square (χ 2) analysis of intrinsic risk factors revealed significantly (P<0.05) higher prevalence of mastitis in crossbred cattle (47.2%) than indigenous (15.4%), in cattle above 7 years (75%) than less than 2-6 years of age (28%) and cows given more than 4 calves (81.3%) than those with less than 4 calves (31.1%) irrespective to their lactation stage. There was also significantly (P<0.05) higher mastitis prevalence in larger (46.6%) than smaller herds (24.2%) and among the farming systems in semi-intensive (47.1%) and intensive (42.3%) than extensive (8.1%) management system. The present study indicated higher prevalence of mastitis linked with several risk factors. Thus, early diagnosis and regular screening of cows for subclinical mastitis together with treatment of clinical cases are of paramount importance. Moreover, control and prevention strategies should be designed and implemented 124 Getachew Kebebew et al.,

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Isolation, identifications and antimicrobial susceptibility pattern of coagulase positive <i>Staphylococcus</i> from subclinical mastitic dairy cattle in and around Haramaya University

Cited by 13 publications

References 13 publications

Isolation, Identification, and Susceptibility Profile of E. coli, Salmonella, and S. aureus in Dairy Farm and Their Public Health Implication in Central Ethiopia

Isolation, Identification, and Susceptibility Profile of E. coli, Salmonella, and S. aureus in Dairy Farm and Their Public Health Implication in Central Ethiopia

Prevalence and Risk Factors of Bovine Mastitis in Southern Zone of Tigray, Northern Ethiopia

Prevalence and risk factors of bovine mastitis in Ambo town of West Shewa Zone, Oromia, Ethiopia

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