Isolation, identification of entomopathogenic nematodes, and preliminary study of their virulence against the great wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae)

Khashaba, Etr H. K.; Moghaieb, Reda E. A.; Azim, Amany M. Abd El; Ibrahim, S. A.

doi:10.1186/s41938-020-00257-6

Cited by 6 publications

(3 citation statements)

References 18 publications

(17 reference statements)

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“…The recovery rate for EPNs at this region was expected to be high due to several factors, as the nature of the soil sample is sandy clay loam and the temperature ranging from 23 to 25°C. This agrees with the findings of Valadas et al (2013) and Khashaba et al (2020); also, the sample size increase the probability of positive samples.…”

Section: Discussionsupporting

confidence: 92%

Genetic variability among three Egyptian isolates of Heterorhabditis indica using a new marker technique (SCoT)

Azim¹,

Khashaba²

2021

Egypt J Biol Pest Control

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Background Entomopathogenic nematodes (EPNs) are a group of nematode families, have the ability to search for their hosts, and are considered as promising biological control candidates for insect pests, providing protection to non-target organisms and the environment. Results This study was conducted to isolate indigenous EPN isolates from Egyptian agricultural soils for further use in biological control programs and study their genetic polymorphism among the previously isolated isolates under accession no. MH553167 and MK300683 and the new isolate (MH496627), using the start codon targeted (SCoT) marker. One out of 15 soil samples obtained from a banana cultivated field was positive for the presence of EPNs, using the Galleria baiting method. Morphological analysis and sequencing of the internal transcribed spacer (ITS) region suggested that the isolate obtained belongs to Heterorhabditis indica. The sequence of the ITS was submitted to the National Center for Biotechnology Information (NCBI) and registered under accession no. MH496627. Ten SCoT primers were used in the study; the polymorphic bands were 68 out of 76 with 89% as polymorphism percentage. The highest numbers of bands were 10 bands generated by SCoT 1 and SCoT 18 while SCoT 48 and SCoT 60 recorded the lowest band number (5 bands). Conclusions The present study is considered as a preliminary study to demonstrate the effectiveness of the SCoT marker for the first time in assessing genetic relationships in EPNs.

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Section: Discussionsupporting

confidence: 92%

Genetic variability among three Egyptian isolates of Heterorhabditis indica using a new marker technique (SCoT)

Azim¹,

Khashaba²

2021

Egypt J Biol Pest Control

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“…At each site, 32 soil samples (500 g each) were collected using a hand shovel, placed into a plastic container, labelled with vegetation and date, and then transferred under ambient temperature to the laboratory for the isolation of EPNs. The soil collection process was performed as described by Khashaba et al [ 39 ]. The soil samples were processed using a baiting technique [ 40 ] with Galleria mellonella larvae followed by a modified White trap [ 41 ] to isolate EPNs.…”

Section: Methodsmentioning

confidence: 99%

Isolation, Identification, and Biocontrol Potential of Entomopathogenic Nematodes and Associated Bacteria against Virachola livia (Lepidoptera: Lycaenidae) and Ectomyelois ceratoniae (Lepidoptera: Pyralidae)

Alotaibi

Darwish

Zaynab

et al. 2022

Biology

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Virachola livia (Lepidoptera: Lycaenidae) and Ectomyelois ceratoniae (Lepidoptera: Pyralidae) are the key pests of pomegranates in Saudi Arabia that are managed mainly using broad-spectrum pesticides. Interactions between entomopathogenic nematodes (EPNs), Steinernematids, Heterorhabditids, and their entomopathogenic bacterial symbionts (EPBs) have long been considered monoxenic 2-partner associations responsible for killing insects and, therefore, are widely used in insect pest biocontrol. However, there are limited reports identifying such organisms in Taif, Saudi Arabia. The current study aimed to identify the EPNs and their associated bacteria isolated from Taif, Saudi Arabia, and evaluate their biocontrol potential on third instars of V. livia and E. ceratoniae larvae under laboratory conditions. A total of 35 EPN isolates belonging to Steinernema (20) and Heterorhabditis (15) were recovered from 320 soil samples. Twenty-six isolates of symbiotic or associated bacteria were isolated from EPNs and molecularly identified as Xenorhabdus (6 isolates), Photorhabdus (4 isolates), Pseudomonas (7), or Stenotrophomonas (9). A pathogenicity assay revealed that Steinernema spp. were more virulent than Heterorhabditis spp. against the two pomegranate insects, with LC50 values of 18.5 and 13.6 infective juveniles (IJs)/larva for Steinernema spp. and 52 and 32.4 IJs/larva for Heterorhabditis spp. at 48 and 72 h post-treatment of V. livia larvae, respectively. Moreover, LC50 values of 9 and 6.6 IJs/larva (Steinernema spp.) and 34.4 and 26.6 IJs/larva (Heterorhabditis spp.) were recorded for E. ceratoniae larvae at 48 and 72 h post-treatment. Moreover, the EPB Stenotrophomonas maltophilia CQ1, isolated from Steinernema spp., surpassed Pseudomonas mosselii SJ10, associated with Heterorhabditis spp., in their ability to kill V. livia or E. ceratoniae larvae within 6 h post-application, resulting in 100% mortality in both insects after 24 and 48 h of exposure. We conclude that applications of either the endogenous EPNs’ IJs or their associated EPB cells could serve as potential biocontrol agents for V. livia and E. ceratoniae.

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“…These baits were placed 15 cm below soil depth near the root zone in a slanting position at 45° angle and were labeled properly and the baiting sites were marked for easy retrieval. Traps were observed on 4th day after initial installation and after 8 days these baits were taken out and inspected for the larval mortality (Khashaba et al 2020). Dead insects were placed on top of filter paper draped inverted watch glass containing sterilized water.…”

Section: Isolation and Maintenance Of Epn Strainsmentioning

confidence: 99%

Efficacy of native strains of entomopathogenic nematode, Heterorhabditis indica against the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) from India

Shinde¹,

Ingole

Biradar³

et al. 2022

Egypt J Biol Pest Control

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Background The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a notorious polyphagous pest that has recently invaded India in 2018. Within a short period, this pest has spread throughout the country, causing a significant damage to maize. In order to manage this menace, farmers rely on chemical insecticides, but due to concealed feeding nature of this pest insecticides remain ineffective in reaching the target site. In this context, the present study aimed to isolate, characterize and evaluate the reproductive potential of the native strains of entomopathogenic nematode, Heterorhabditis indica against larval instars of S. frugiperda under laboratory conditions. Results Two strains of H. indica isolated and characterized from the rhizosphere of mango and curry leaf trees were designated as CICR-HI-MN and CICR-HI-CL, respectively. Both strains, CICR-HI-CL and CICR-HI-MN showed differential mortality against 3rd (LC50 value of 21.65, 48.91 IJs/larva), 4th (LC50 value of 25.46, 52.36 IJs/larva) and 5th (LC50 value of 59.20, 71.04 IJs/larva) instar larvae of S. frugiperda, respectively. The instar-wise mortality of S. frugiperda showed that 3rd instar was relatively more susceptible compared to 4th and 5th instar larvae. In terms of efficacy, CICR-HI-CL was almost twice effective than CICR-HI-MN when LC50 and LC95 values of 3rd instar larvae were compared. However, the reproductive potential of both the strains was significantly higher against 5th instar than the 3rd and 4th instar larvae. Strain CICR-HI-CL was more effective in larval mortality, while nematode reproduction was higher in CICR-HI-MN. Conclusions Native strains of H. indica evaluated in the present study were found effective against larval instars of fall armyworm, S. frugiperda. Higher reproduction on 5th instar larvae demonstrates the ability of these strains to multiply, sustain and perpetuate on late instars of this polyphagous pest and may serve as viable option in integrated pest management program.

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Isolation, identification of entomopathogenic nematodes, and preliminary study of their virulence against the great wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae)

Cited by 6 publications

References 18 publications

Genetic variability among three Egyptian isolates of Heterorhabditis indica using a new marker technique (SCoT)

Genetic variability among three Egyptian isolates of Heterorhabditis indica using a new marker technique (SCoT)

Isolation, Identification, and Biocontrol Potential of Entomopathogenic Nematodes and Associated Bacteria against Virachola livia (Lepidoptera: Lycaenidae) and Ectomyelois ceratoniae (Lepidoptera: Pyralidae)

Efficacy of native strains of entomopathogenic nematode, Heterorhabditis indica against the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) from India

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