Genetic variability among three Egyptian isolates of Heterorhabditis indica using a new marker technique (SCoT)

Abstract

Background Entomopathogenic nematodes (EPNs) are a group of nematode families, have the ability to search for their hosts, and are considered as promising biological control candidates for insect pests, providing protection to non-target organisms and the environment. Results This study was conducted to isolate indigenous EPN isolates from Egyptian agricultural soils for further use in biological control programs and study their genetic polymorphism among the previously isolated isolates under accession no. MH553167 and MK300683 and the new isolate (MH496627), using the start codon targeted (SCoT) marker. One out of 15 soil samples obtained from a banana cultivated field was positive for the presence of EPNs, using the Galleria baiting method. Morphological analysis and sequencing of the internal transcribed spacer (ITS) region suggested that the isolate obtained belongs to Heterorhabditis indica. The sequence of the ITS was submitted to the National Center for Biotechnology Information (NCBI) and registered under accession no. MH496627. Ten SCoT primers were used in the study; the polymorphic bands were 68 out of 76 with 89% as polymorphism percentage. The highest numbers of bands were 10 bands generated by SCoT 1 and SCoT 18 while SCoT 48 and SCoT 60 recorded the lowest band number (5 bands). Conclusions The present study is considered as a preliminary study to demonstrate the effectiveness of the SCoT marker for the first time in assessing genetic relationships in EPNs.

ISSR and SCoT for evaluation of hereditary differences of 29 wild plants in Al Jubail Saudi Arabian

“…The separation of the inspected species of unmistakable increases may be caused by diverse biological conditions, which might contribute to expanding the hereditary variety between species, which might contribute to growing the innate assortment between species. These results were agreed with other reports which used SCoT markers in estimating the genetic relationships, as in Tritordeum bergrothii L. - Poaceae ( Cabo et al, 2014 ), Saudi Arabian date palm cultivars ( Al-Qurainy et al, 2015 ), between Chinese Elymus sibricus L. - Poaceae accessions ( Zhang et al, 2015 ), between 14 cultivars of T. aestivum L. related to different Northern African countries ( Mohamed et al, 2017 ), and among indigenous EPNs belonging to H. indica species ( Abd El Azim and Khashaba, 2021 ). Hereditary characterizations of the halophyte Suaeda maritima in Thailand given by SCoT profiles ( Rittirongsakul et al, 2020 ), and cultivar characters based on hereditary solidness with SCoT molecular marker in modern new Polish lines of Chenopodium quinoa Willd ( Lema-Rumińska et al, 2018 ).…”