Isolation, Identification and Tyrosinase Inhibitory Activities of the Extractives from Allamanda cathartica

Yamauchi, Kosei; Mitsunaga, Tohru; Batubara, Irmanida

doi:10.4236/nr.2011.23022

Cited by 29 publications

(21 citation statements)

References 14 publications

(17 reference statements)

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“…It has been used as a purgative or emetic, febrifuge, as well as for the treatment of coughs, headaches, jaundice and enlarged spleen resulting from malaria. The milky sap is also known to possess antibacterial and possibly anticancer properties [12,13] . Bixa orellana L. (Bixaceae) (B. orellana) is a tropical shrub, with a bright red heart shaped, bristly fruit and seeds embedded in orange-red pulp.…”

Section: Introductionmentioning

confidence: 99%

In vivo antioxidant assessment of two antimalarial plants–Allamamda cathartica and Bixa orellana

Conrad

Dike

Agbara

2013

Asian Pacific Journal of Tropical Biomedicine

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Section: Introductionmentioning

confidence: 99%

In vivo antioxidant assessment of two antimalarial plants–Allamamda cathartica and Bixa orellana

Conrad

Dike

Agbara

2013

Asian Pacific Journal of Tropical Biomedicine

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“…4, one of the flavonol and widely distributed in plants including onion, guava leaves and seaweeds, exhibits potent tyrosinase inhibitory activity [38,39]. Glabridin, an isoflavan derivatives, was isolated from Allamanda cathartica stem as potent tyrosinase inhibitor [40,41]. Artocarbene, chlorophorin, norartocarpanone, and 4-propylresorcinol including 2,4-substituted resorcinol moiety induce high tyrosinase inhibitory activity [42,43].…”

Section: Melanogenesis Controlling Effect Of Medicinal Plants Extractmentioning

confidence: 99%

Effect of quercetin derivatives on melanogenesis stimulation of melanoma cells

Mitsunaga

Yamauchi

2015

J Wood Sci

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Controlling melanogenesis is important for maintaining the good health and cosmetic appearance of a human body. This study aims to search the active compounds from natural products exhibiting the melanogenesis modulating activity and elucidate the mechanism underlying the observed activity. Two novel quercetin glycosideswere isolated and identified from Helminthostachys zeylanica roots 50 % ethanol extract. Compound 1 exhibited intracellular melanogenesis stimulatory activity, while 2 showed no effect even the structural similarity. To understand the structure-activity relationships, twelve quercetin glycosides and seven methylquercetins were synthesized from rutin as starting material. As the result of bioassay using synthesized nineteen quercetin derivatives in B16 melanoma cells, some quercetin-3-O-b-D-glucopyranosides stimulated the intracellular melanogenesis. On the other hand, synthesized 3-O-methylquercetin 12 and 3,4 0 ,7-Otrimethylquercetin 15 increased both intra and extracellular melanin contents with no cytotoxicity. Compoud 15 increased the phosphorylated p38 mitogen-activated protein kinase (MAPK) and microphthalmia-associated transcription factor (MITF) which regulates the expression of tyrosinase, TRP-1 and TRP-2. While 12 enhanced the expression of the melanogenic enzymes without involving the MITF, as evidenced by its lack of any stimulation of the expression of MITF and p-p38 MAPK. This result indicates that 12 may stimulate the expression of tyrosinase, TRP-1, and TRP-2 by stimulating currently unidentified transcriptional factors and/or by regulating the degradation of melanogenic enzymes.

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“…The reaction was performed as previously described in a 50 mM phosphate buffer at pH 6.8, with 0.5 mM L-tyrosine, 10 mg/mL of kojic acid, and 5000 U/mL of mushroom tyrosinase at 30 • C [42]. Experimental conditions were also based on previous studies [44,45]. After incubation, absorbance were measured at 450 nm every 2 min, for a total of 10 min using 96-well reader [44].…”

Section: Acetylcholinesterase and Tyrosinase Activitymentioning

confidence: 99%

Supercritical CO2 Extracts and Volatile Oil of Basil (Ocimum basilicum L.) Comparison with Conventional Methods

et al. 2018

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Interest in new products from aromatic plants as medical and nutritional compounds is increasing. The aim of this work was to apply different extraction methods, including the use of supercritical carbon dioxide extraction, and to test the antioxidant activity of basil (Ocimum basilicum L.) extracts. In vitro efficacy assessments were performed using enzymatic assays. Essential oil obtained by hydrodistillation and volatile oil obtained from supercritical fluid extraction were analyzed by gas chromatography to quantify components. The total phenolic content in the extracts ranged from 35.5 ± 2.9 to 85.3 ± 8.6 mg of gallic acid equivalents and the total flavonoid content ranged from 35.5 ± 2.9 to 93.3 ± 3.9 micromole catechin equivalents per gram of dry weight of extract. All the extracts showed an antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), and the reducing power test. Extracts obtained from methanol had a higher antioxidant capacity per the DPPH test results (IC 50 = 3.05 ± 0.36 mg/mL) and the reducing power test assay 306.8 ± 21.8 µmol of trolox equivalents per gram of extract (TE/g) compared with ethanolic or supercritical fluid extracts. However, using the ABTS assay, the extract obtained by supercritical fluid extraction had a higher antioxidant capacity with an IC 50 of 1.74 ± 0.05 mg/mL. Finally, the examined extracts showed practically no acetylcholinesterase (AChE) inhibitory capacity and a slight inhibitory activity against tyrosinase.

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Isolation, Identification and Tyrosinase Inhibitory Activities of the Extractives from Allamanda cathartica

Abstract: ABSTRACT

Cited by 29 publications

References 14 publications

In vivo antioxidant assessment of two antimalarial plants–Allamamda cathartica and Bixa orellana

In vivo antioxidant assessment of two antimalarial plants–Allamamda cathartica and Bixa orellana

Effect of quercetin derivatives on melanogenesis stimulation of melanoma cells

Supercritical CO2 Extracts and Volatile Oil of Basil (Ocimum basilicum L.) Comparison with Conventional Methods

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