Isolation, Identification and Partial Characterization of a Lactobacillus casei Strain with Bile Salt Hydrolase Activity from Pulque

Abstract

The aim of this study was to isolate, from pulque, Lactobacillus spp. capable of survival in simulated gastrointestinal stress conditions. Nine Gram-positive rods were isolated; however, only one strain (J57) shared identity with Lactobacillus and was registered as Lactobacillus casei J57 (GenBank accession: JN182264). The other strains were identified as Bacillus spp. The most significant observation during the test of tolerance to simulated gastrointestinal conditions (acidity, gastric juice and bile salts) was that L. casei J57 showed a rapid decrease (p ≤ 0.05) in the viable population at 0 h. Bile salts were the stress condition that most affected its survival, from which deoxycholic acid and the mix of bile salts (oxgall) were the most toxic. L. casei J57 showed bile salt hydrolase activity over primary and secondary bile salts as follows: 44.91, 671.72, 45.27 and 61.57 U/mg to glycocholate, taurocholate, glycodeoxycholate and taurodeoxycholate. In contrast, the control strain (L. casei Shirota) only showed activity over tauroconjugates. These results suggest that L. casei J57 shows potential for probiotic applications.

Pulque, a Traditional Mexican Alcoholic Fermented Beverage: Historical, Microbiological, and Technical Aspects

“…Antimicrobial activity against pathogenic bacteria such as enteropathogenic Escherichia coli, Salmonella enterica serovar Typhimurium, S. enterica serovar Typhi and Listeria monocytogenes (Castro-Rodríguez et al, 2015; González-Vázquez et al, 2015; Giles-Gómez et al, 2016; Torres-Maravilla et al, 2016). …”

“…He reported in the Boletin de la Sociedad de Geografía y Estadística , the isolation of Termobacterium mobile by Paul Lindner in 1924 (Weir, 2016), among others. Pulque 's microbiology, the isolation of strains, and more recently, its individual probiotic characterization, is probably the main research trend (Torres-Rodríguez et al, 2014; Castro-Rodríguez et al, 2015; González-Vázquez et al, 2015; Giles-Gómez et al, 2016; Torres-Maravilla et al, 2016). An additional research subject deals with the effect of pulque in the Mexican diet.…”

“…Emerging research on the microbiology of pulque focuses on the isolation and in vitro as in vivo assessment of probiotic LAB with promising capabilities (Castro-Rodríguez et al, 2015; González-Vázquez et al, 2015; Giles-Gómez et al, 2016; Reyes-Naya et al, 2016; Torres-Maravilla et al, 2016; Table 4). …”

In vitro assessment of hypocholesterolemic activity of Lactococcus lactis subsp. lactis

“…Lactobacillus spp. from the stationary phase of static cultures had high BSH activities at low pH values (González-Vázquez et al 2015;Sedláčková et al 2015).…”

“…Recently, several studies have been published on the distribution and activity of BSH in more than 300 lactic acid bacteria strains from the genera Bifidobacterium and Lactobacillus, and the species Lactococcus lactis, Leuconostoc mesenteroides, and Streptococcus thermophilus (González-Vázquez et al 2015;Xu et al 2016;Rani et al 2017). Lactobacillus spp.…”