Isolation, identification, and fenvalerate-degrading potential of <i>Bacillus licheniformis</i> CY-012

Tang, Jie; Liu, Bo; Shi, Ying; Zeng, Chaoyi; Chen, Tingting; Zeng, Lin; Zhang, Qing

doi:10.1080/13102818.2018.1438210

Cited by 22 publications

(16 citation statements)

References 35 publications

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“…Subsequently, 5 ml medium aliquots were transferred to new Erlenmeyer flasks containing 50 ml LB/MM medium and different DBP concentrations (50,100,200, 400, 800, 1,600 mg/l). After six rounds of culturing, the final suspensions were diluted, plated, and incubated on MM agar plates containing 50 mg/l DBP [1] to obtain a single colony using DBP as the sole carbon and energy source. The degradation ability of the isolates was confirmed using high-performance liquid chromatography (HPLC).…”

Section: Enrichment Isolation and Identification Of Dbp-degrading Strainmentioning

confidence: 99%

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

Tang

Zhou

et al. 2022

J. Microbiol. Biotechnol.

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Continued fenvalerate use has caused serious environmental pollution and requires large-scale remediation. Dibutyl phthalate (DBP) was discovered in fenvalerate metabolites degraded byCitrobacter freundii CD-9. Coculturing is an effective method for bioremediation, but few studies have analyzed the degradation pathways and potential mechanisms of cocultures. Here, a DBPdegrading strain (BDBP 071) was isolated from soil contaminated with pyrethroid pesticides (PPs) and identified as Stenotrophomonas acidaminiphila. The optimum conditions for DBP degradation were determined by response surface methodology (RSM) analysis to be 30.9 mg/l DBP concentration, pH 7.5, at a culture temperature of 37.2°C. Under the optimized conditions, approximately 88% of DBP was degraded within 48 h and five metabolites were detected. Coculturing C. freundii CD-9 and S. acidaminiphila BDBP 071 promoted fenvalerate degradation. When CD-9 was cultured for 16 h before adding BDBP 071, the strain inoculation ratio was 5:5 (v/v), fenvalerate concentration was 75.0 mg/l, fenvalerate was degraded to 84.37 ± 1.25%, and DBP level was reduced by 5.21 mg/l. In addition, 12 fenvalerate metabolites were identified and a pathway for fenvalerate degradation by the cocultured strains was proposed. These results provide theoretical data for further exploration of the mechanisms used by this coculture system to degrade fenvalerate and DBP, and also offer a promising method for effective bioremediation of PPs and their related metabolites in polluted environments.

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Section: Enrichment Isolation and Identification Of Dbp-degrading Strainmentioning

confidence: 99%

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

Tang

Zhou

et al. 2022

J. Microbiol. Biotechnol.

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“…were isolated from Brazilian savannah, sea, and the tropical peat normally described as “turfa” soil to accelerate the degradation of esfenvalerate and a biometabolic pathway was proposed (Birolli et al 2016 ). In a separate study, a strain of Bacillus licheniformis CY-012 was isolated from soil in a garden that had been sprayed with pyrethroids, and the bacteria was found to efficiently degrade 80% fenvalerate approximately (Tang et al 2018 ). Citrobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9

et al. 2022

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Citrobacter freundii CD-9 is a Gram-negative bacteria sourced from factory sludge that can use fenvalerate as its sole carbon source and has a broad degradation spectrum for pyrethroid pesticides. The whole genome of CD-9 sequenced using Illumina HiSeq PE150 was reported in this study. The CD-9 genome size was 5.33 Mb and the G + C content was 51.55%. A total of 5291 coding genes, 9 5s-rRNA, and 79 tRNA were predicted bioinformatically. 3586 genes annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that can be involved in 173 metabolic pathways, including various microbial metabolic pathways that degrade exogenous chemicals, especially those that degrade aromatic compounds, and also produce a variety of bioactive substances. Fifty genes related to pyrethroid degradation were identified in the C. freundii CD-9 genome, including 9 dioxygenase, 25 hydrolase, and 16 esterase genes. Notably, RT-qPCR results showed that from the predicted 13 genes related to fenvalerate degradation, the expression of six genes, including esterase, HAD family hydrolase, lipolytic enzyme, and gentisic acid dioxygenase, was induced in the presence of fenvalerate. In this study, the key genes and degradation mechanism of C. freundii CD-9 were analyzed and the results provide scientific evidence to support its application in environmental bioremediation. It can establish application models for different environmental pollution management by constructing genetically engineered bacteria for efficient fenvalerate or developing enzyme formulations that can be industrially produced.

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“…In a separate study, a strain of Bacillus licheniformis CY-012 was isolated from soil in a garden that had been sprayed with pyrethroids, and the bacteria was found to e ciently degrade 80% fenvalerate approximately. (Tang et al 2018). Citrobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Whole Genome Sequencing and Analysis of Fenvalerate Degrading Bacteria Citrobacter freundii CD-9

Zhou

Lei

Tang

et al. 2022

Preprint

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Citrobacter freundii CD-9 is a gram-negative bacteria sourced from factory sludge that can use fenvalerate as its sole carbon source and has a broad degradation spectrum for pyrethroid pesticides. The whole genome of CD-9 sequenced using Illumina HiSeq PE150 was reported in this study. The CD-9 genome size was 5.33 Mb and the G + C content was 51.55%. A total of 5,291 coding genes, 9 5s- rRNA, and 79 tRNA were predicted bioinformatically. 3586 genes annotated to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that can be involved in 173 metabolic pathways, including various microbial metabolic pathways that degrade exogenous chemicals, especially those that degrade aromatic compounds, and also produce a variety of bioactive substances. Fifty genes related to pyrethroid degradation were identified in the C. freundii CD-9 genome, including 9 dioxygenase, 25 hydrolase, and 16 esterase genes. Notably, RT-qPCR results showed that from the predicted 13 genes related to fenvalerate degradation, the expression of six genes, including esterase, HAD family hydrolase, lipolytic enzyme, and gentisic acid dioxygenase, was induced in the presence of fenvalerate. In this study, the key genes and degradation mechanism of C. freundii CD-9 were analyzed and the results provide scientific evidence to support its application in environmental bioremediation.

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Isolation, identification, and fenvalerate-degrading potential of Bacillus licheniformis CY-012

Cited by 22 publications

References 35 publications

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate

Whole genome sequencing and analysis of fenvalerate degrading bacteria Citrobacter freundii CD-9

Whole Genome Sequencing and Analysis of Fenvalerate Degrading Bacteria Citrobacter freundii CD-9

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