Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil

Nandhini, S.; Sudha, S.; Jeslin, V. Anusha; Sharma, Mohan Lal

doi:10.1016/j.bcab.2018.06.024

Cited by 25 publications

(16 citation statements)

References 11 publications

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“…In fact, the capability of solvents used for extraction from cell free culture filtrates of Streptomycetes spp. differs according to both solvents used and chemical nature of the antifungal agent produced [43]. These results support previous studies in this respect [44].…”

Section: Discussionsupporting

confidence: 90%

Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

Abdel-Shafi

Al-Mohammadi²,

Almanaa

et al. 2020

Antibiotics

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There is a need to continue research to find out other anti-dermatophytic agents to inhibit causal pathogenic skin diseases including many types of tinea. We undertook the production, purification, and identification of an anti-dermatophytic substance by Streptomyces atrovirens. Out of 103 streptomycete isolates tested, only 20 of them showed antidermatophytic activity with variable degrees against Trichophyton tonsurans CCASU 56400 (T. tonsurans), Microsporum canis CCASU 56402 (M. canis), and Trichophyton mentagrophytes CCASU 56404 (T. mentagrophytes). The most potent isolate, S10Q6, was identified based on the tests conducted that identified morphological and physiological characteristics and using 16S rRNA gene sequencing. The isolate was found to be closely correlated to previously described species Streptomyces atrovirens; it was designated Streptomyces atrovirens KM192347 (S. atrovirens). Maximum antifungal activity of the strain KM192347 was obtained in modified starch nitrate medium (MSNM) adjusted initially at pH 7.0 and incubated at 30 °C in shaken cultures (150 rpm) for seven days. The antifungal compound was purified by using two steps protocol including solvent extraction and column chromatography. The MIC of it was 20 µg/mL against the dermatophyte cultures tested. According to the data obtained from instrumental analysis and surveying the novel antibiotics database, the antidermatophytic substance produced by the strain KM192347 was characterized as an oxaborole-6-benzene sulphonamide derivative and designated oxaborole-6-benzene sulphonamide (OXBS) with the chemical formula C13H12 BNO4S. The crude OXBS didn’t show any toxicity on living cells. Finally, the results obtained herein described another anti-dermatophytic substance named an OXBS derivative.

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Section: Discussionsupporting

confidence: 90%

Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

Abdel-Shafi

Al-Mohammadi²,

Almanaa

et al. 2020

Antibiotics

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“…Various methods to detect fungal growth inhibition in vitro , such as the pairwise challenge assays performed in this study, have been successfully developed and applied in the past [53,54,73,74]. In vitro screening assays, such as these FBI, can be useful in selecting microbial antagonists for in vivo experiments.…”

Section: Discussionmentioning

confidence: 99%

Valley Fever on the Rise—Searching for Microbial Antagonists to the Fungal Pathogen Coccidioides immitis

et al. 2019

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The incidence of coccidioidomycosis, also known as Valley Fever, is increasing in the Southwestern United States and Mexico. Despite considerable efforts, a vaccine to protect humans from this disease is not forthcoming. The aim of this project was to isolate and phylogenetically compare bacterial species that could serve as biocontrol candidates to suppress the growth of Coccidioides immitis, the causative agent of coccidioidomycosis, in eroded soils or in areas close to human settlements that are being developed. Soil erosion in Coccidioides endemic areas is leading to substantial emissions of fugitive dust that can contain arthroconidia of the pathogen and thus it is becoming a health hazard. Natural microbial antagonists to C. immitis, that are adapted to arid desert soils could be used for biocontrol attempts to suppress the growth of the pathogen in situ to reduce the risk for humans and animals of contracting coccidioidomycosis. Bacteria were isolated from soil samples obtained near Bakersfield, California. Subsequently, pairwise challenge assays with bacterial pure cultures were initially performed against Uncinocarpus reesii, a non-pathogenic relative of C. immitis on media plates. Bacterial isolates that exhibited strongly antifungal properties were then re-challenged against C. immitis. Strongly anti-C. immitis bacterial isolates related to Bacillus subtilis and Streptomyces spp. were isolated, and their antifungal spectrum was investigated using a selection of environmental fungi.

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“…is the primary natural source of bioactive secondary metabolites of great importance, such as antifungals, antivirals, antitumors, antihypertensives, immunosuppressants, and mainly antibiotics (McKenzie et al, 2010;de Lima Procópio et al, 2012). The genus Streptomyces alone accounts for approximately 75% of all bioactive compounds, including antibiotics (Usha Nandhini et al, 2018). The ability of this genus to produce antibiotics and other secondary metabolites has been an evolutionary advantage, facilitating its adaptation to different and variable stress situations.…”

Section: Generalities Of the Streptomyces Genusmentioning

confidence: 99%

Application of Streptomyces Antimicrobial Compounds for the Control of Phytopathogens

Pacios‐Michelena

González

Álvarez-Pérez

et al. 2021

Front. Sustain. Food Syst.

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One of the relevant problems in today's agriculture is related to phytopathogenic microorganisms that cause between 30–40% of crop losses. Synthetic chemical pesticides and antibiotics have brought human and environmental health problems and microbial resistance to these treatments. So, the search for natural alternatives is necessary. The genus Streptomyces have broad biotechnological potential, being a promising candidate for the biocontrol of phytopathogenic microorganisms. The efficacy of some species of this genus in plant protection and their continued presence in the intensely competitive rhizosphere is due to its great potential to produce a wide variety of soluble bioactive secondary metabolites and volatile organic compounds. However, more attention is still needed to develop novel formulations that could increase the shelf life of streptomycetes, ensuring their efficacy as a microbial pesticide. In this sense, encapsulation offers an advantageous and environmentally friendly option. The present review aims to describe some phytopathogenic microorganisms with economic importance that require biological control. In addition, it focuses mainly on the Streptomyces genus as a great producer of secondary metabolites that act on other microorganisms and plants, exercising its role as biological control. The review also covers some strategies and products based on Streptomyces and the problems of its application in the field.

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Isolation, identification and extraction of antimicrobial compounds produced by Streptomyces sps from terrestrial soil

Cited by 25 publications

References 11 publications

Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

Valley Fever on the Rise—Searching for Microbial Antagonists to the Fungal Pathogen Coccidioides immitis

Application of Streptomyces Antimicrobial Compounds for the Control of Phytopathogens

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