Identification and Testing of Antidermatophytic Oxaborole-6-Benzene Sulphonamide Derivative (OXBS) from Streptomyces atrovirens KM192347 Isolated from Soil

Abstract

There is a need to continue research to find out other anti-dermatophytic agents to inhibit causal pathogenic skin diseases including many types of tinea. We undertook the production, purification, and identification of an anti-dermatophytic substance by Streptomyces atrovirens. Out of 103 streptomycete isolates tested, only 20 of them showed antidermatophytic activity with variable degrees against Trichophyton tonsurans CCASU 56400 (T. tonsurans), Microsporum canis CCASU 56402 (M. canis), and Trichophyton mentagrophytes CCASU 56404 (T. mentagrophytes). The most potent isolate, S10Q6, was identified based on the tests conducted that identified morphological and physiological characteristics and using 16S rRNA gene sequencing. The isolate was found to be closely correlated to previously described species Streptomyces atrovirens; it was designated Streptomyces atrovirens KM192347 (S. atrovirens). Maximum antifungal activity of the strain KM192347 was obtained in modified starch nitrate medium (MSNM) adjusted initially at pH 7.0 and incubated at 30 °C in shaken cultures (150 rpm) for seven days. The antifungal compound was purified by using two steps protocol including solvent extraction and column chromatography. The MIC of it was 20 µg/mL against the dermatophyte cultures tested. According to the data obtained from instrumental analysis and surveying the novel antibiotics database, the antidermatophytic substance produced by the strain KM192347 was characterized as an oxaborole-6-benzene sulphonamide derivative and designated oxaborole-6-benzene sulphonamide (OXBS) with the chemical formula C13H12 BNO4S. The crude OXBS didn’t show any toxicity on living cells. Finally, the results obtained herein described another anti-dermatophytic substance named an OXBS derivative.

Inhibition of Staphylococcus aureus LC 554891 by Moringa oleifera Seed Extract either Singly or in Combination with Antibiotics

“…The controls were filter paper discs soaked in sterile distilled water. Samples and controls were incubated at 37 °C for 24–48 h. IZDs were measured after subtracting the diameter of the paper disc [ 17 , 21 , 64 ].…”

Powerful Antibacterial Peptides from Egg Albumin Hydrolysates

“…Bioactive peptides can be obtained from different protein sources, including milk [ 27 ] and legume proteins [ 28 , 29 ]. Peptides from various protein resources have been reported to have multiple functions, including antioxidative ability, angiotensin I converting enzyme (ACE) inhibitor properties [ 30 ], hepatoprotective activity [ 31 ], and antibacterial activity [ 6 , 19 , 20 , 32 , 33 , 34 , 35 , 36 ].…”

Silver Nanoparticles (AgNPs) Biosynthesized by Aspergillus flavus KF946095; their Characterization and Antibacterial Activity

“…The detection of a newly research for suppressing and blocking the various impedance microscopic organisms to antibiotic is urgent (Enan et al, 2013;Enan et al, 2014;Abdel-Shafi et al, 2020). Nanotechnology introduce effectiveness ways in inhibition of pathogenic multidrug resistant bacteria (Gu et al, 2003; El-Gazzar and Ismail 2020).The emergence of nanotechnology in the last decade offers recent biotechnology in development of drugs based nanoparticles (El-Gazzar and Enan, 2020).…”