Isolation, Identification and Evaluation of Bacterial Antagonists against <i>Botrytis elliptica</i> on Lily

Chiou, Aih-Jing; Wu, Wen-Jong

doi:10.1046/j.1439-0434.2001.00627.x

Cited by 35 publications

(27 citation statements)

References 33 publications

(34 reference statements)

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“…In relation to B. gladioli that was only recovered from enrichment isolation and resistant to glyphosate, Shimosaka et al (2000) observed quitinase and quitosanase production by this species that may also be interesting for biological control. Chiou and Wu (2001) observed that B. gladioli strains presented antagonistic effects against Botrytis elliptica on three lily cultivars in greenhouse trials. Furthermore, other Burkholderia species have potential application in biological control and plant growth promotion (Chen et al, 1995;Heydari and Misaghi, 1998;Heydari et al, 1997;Huang and Wong, 1998;Traˆn Van et al, 2000).…”

Section: Discussionmentioning

confidence: 95%

Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide

et al. 2005

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Endophytic bacteria are ubiquitous in most plant species influencing the host fitness by disease suppression, contaminant degradation, and plant growth promotion. This endophytic bacterial community may be affected by crop management such as the use of chemical compounds. For instance, application of glyphosate herbicide is common mainly due to the use of glyphosate-resistant transgenic plants. In this case, the bacterial equilibrium in plant-endophyte interaction could be shifted because some microbial groups are able to use glyphosate as a source of energy and nutrients, whereas this herbicide may be toxic to other groups. Therefore, the aim of this work was to study cultivable and noncultivable endophytic bacterial populations from soybean (Glycine max) plants cultivated in soil with and without glyphosate application (preplanting). The cultivable endophytic bacterial community recovered from soybean leaves, stems, and roots included Acinetobacter calcoaceticus, A. junii, Burkholderia sp., B. gladioli, Enterobacter sakazaki, Klebsiella pneumoniae, Pseudomonas oryzihabitans, P. straminea, Ralstonia pickettii, and Sphingomonas sp. The DGGE (Denaturing Gradient Gel Electrophoresis) analysis from soybean roots revealed some groups not observed by isolation that were exclusive for plants cultivated in soil with pre-planting glyphosate application, such as Herbaspirillum sp., and other groups in plants that were cultivated in soil without glyphosate, such as Xanthomonas sp. and Stenotrophomonas maltophilia. Furthermore, only two bacterial species were recovered from soybean plants by glyphosate enrichment isolation. They were Pseudomonas oryzihabitans and Burkholderia gladioli which showed different sensibility profiles to the glyphosate. These results suggest that the application at pre-planting of the glyphosate herbicide may interfere with the endophytic bacterial community's equilibrium. This community is composed of different species with the capacity for plant growth promotion and biological control that may be affected. However, the evaluation of this treatment in plant production should be carried out by long-term experiments in field conditions.

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Section: Discussionmentioning

confidence: 95%

Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide

et al. 2005

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“…Several antifungal compounds synthesized by Bacillus species are active against filamentous fungi and yeasts. In a study conducted by Chiou and Wu (2001), several antagonistic bacteria were selected and examined for their biocontrol activities. Bacillus subtilis has been used as a biological control agent to control Fusarium moniliforme (Sakai et al 1998;Bacon et al 2001), and is known to have antagonistic activities against several other plant pathogenic fungi such as R. solani, Pythium ultimum (Kim et al 1997) and Botrytis cinerea (Walker et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Antifungal chitinases from Bacillus pumilus SG2: preliminary report

Ghasemi

Ahmadian

Jelodar

et al. 2010

World J Microbiol Biotechnol

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A halotolerant bacterium capable of chitinase production was previously isolated from saline soils of Gavkhooni marsh, Iran. The strain was identified as Bacillus pumilus strain SG2. This strain secretes two chitinases into the medium in response to the presence of chitin in the medium. The two chitinases, ChiS and ChiL, were active on both polymeric as well as oligosaccharide substrates. In this study, these two extracellular chitinases were purified from the supernatant of the Bacillus culture by ammonium sulfate precipitation. The optimum pH and temperature for both of these chitinases were pH 6 and 37°C, respectively. According to the conserved domain analysis, ChiS and ChiL were categorized in family 18 of the glycosylhydrolases. Three essential conserved amino acid residues in in ChiL were found within the active site. Antifungal activities of the two purified chitinases were assessed on Rhizoctonia solani, Verticillium sp., Nigrospora sp., Stemphyllium botryosum and Bipolaris sp. demonstrating inhibition of all the tested strains. However, these chitinases did not inhibit cell wall formation of the non-chitin-containing fungi (oomycetes) Phytophthora citricola and Phytophthora capsici. This is the first investigation demonstrating antifungal activity of chitinases purified from B. pumilus SG2.

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“…Leaves without antagonistic bacteria served as a control in this experiment. Each treatment consisted of three detached leaves in three replications (Chiou and Wu 2001).…”

Section: Identification Of the Efficient Isolates Based On 16s Rdna Hmentioning

confidence: 99%

Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis

Lee

Kamala‐Kannan

Sub

et al. 2007

World J Microbiol Biotechnol

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Phytophthora blight is one of the most important devastating diseases of red pepper plants. Forty-one bacterial isolates were obtained from rhizosphere soil and subsequently tested for antagonistic activity under in vitro and in vivo conditions. Among the 41 isolates tested, 12 exhibited a maximum antagonistic activity in dual culture assay. These 12 isolates were further screened for disease suppression on red pepper plants in both natural and greenhouse conditions. All the antagonists showed varying levels of antagonism, whereas the isolates R33 and R13 exhibited the maximum (86.8 and 71%) ability to reduce the disease severity in in vivo conditions. Based on the 16S rDNA sequencing, the most effective isolates were identified as Bacillus subtilis. In addition, the isolates were also screened for siderophores, hydrogen cyanide and hydrolytic enzymes. Further, the isolates increased the root and shoot length of the red pepper, which is an added advantage of the isolates while performing the desired function.

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Isolation, Identification and Evaluation of Bacterial Antagonists against Botrytis elliptica on Lily

Cited by 35 publications

References 33 publications

Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide

Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide

Antifungal chitinases from Bacillus pumilus SG2: preliminary report

Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis

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