Isolation, identification and characterization of algicidal bacteria against Stephanodiscus hantzschii and Peridinium bipes for the control of freshwater winter algal blooms

Abstract

Five strains (HYY0510-SK04, HYY0511-SK09, HYK0512-SK12, HYK0512-PK04 and HYY0512-PK05) of algicidal bacteria against the harmful bloom forming diatom Stephanodiscus hantzschii and dinoflagellate Peridinium bipes, were isolated. Among these strains, HYY0510-SK04, HYY0511-SK09 and HYK0512-SK12 have an effective algicidal activity for S. hantzschii, while HYK0512-PK04 and HYY0512-PK05 have an algicidal effect against P. bipes. Sequence analysis of 16S rDNA showed that HYY0510-SK04 and HYY0511-SK09 were closely related to Acidovorax delafieldii ATCC 17505 T . HYK0512-SK12, HYK0512-PK04 and HYY0512-PK05 showed high homology with Variovorax paradoxus IAM 12373 T (98.9%), Hydrogenophaga palleronii ATCC 49743 T (98.8%) and Pseudomonas plecoglossicida ATCC 700383 T (98.3%), respectively. HYY0510-SK04, HYY0511-SK09 and HYK0512-SK12 degraded S. hantzschii cells within two weeks when those bacteria were inoculated at densities of ≥10 7 cells mL −1 to the lag or logarithmic growth phase of the algal culture. HYK0512-PK04 and HYY0512-PK05 degraded more than 90% of P. bipes cells within 14 and 8 days, respectively, when these bacteria were inoculated at densities of ≥10 7 cells mL −1 . Among the five bacterial strains, HYK0512-SK12 and HYY0512-PK05 showed the most effective growth inhibition of all the algae and cyanobacteria tested. Biochemical assays revealed that the main algicidal substance from all isolates were likely to be extracellular substances. These results indicate that the bacterial strains isolated for this study are potential agents for the control of harmful algal blooms in eutrophic reservoirs.

Characterisation of algicidal bacterial exometabolites against the lipid-accumulating diatom Skeletonema sp.

“…The existence of naturallyoccurring lytic agents which could be used to disrupt microalgal blooms is of increasing interest in the developing field of environmental biotechnology. The induction of algal cell lysis by viruses [3] and bacteria [4] is a well-known phenomenon and could provide a low-cost natural method for disrupting cell structures and so controlling microalgal growth. The recent interest in large-scale growth of microalga for the production of bulk and speciality chemicals, including biofuels, provides a further interest in developing low cost lysis methods which could be adapted for use in bioprocessing [5].…”

The antialgal activity of 40 medicinal plants against Microcystis aeruginosa

“…Chemical agents such as copper sulfate (Han et al 2001) and hydrogen peroxide (Drabkova et al 2007) are effective in controlling blooms within a short period after application, but their usage in aquatic ecosystems is potentially dangerous (Jeong et al 2000). Biological agents, such as bacteria (Kang et al 2008), viruses (Yoshida et al 2006), planktonic ciliates (Mayali and Doucette 2002), and fungi (Sigee et al 1999), have disadvantages such as difficulty of application, and in some cases, they have the potential of disastrous ecological consequences. None of these methods can specifically control harmful algal blooms without causing problems towards other organisms.…”

Acute Toxicity Assessment of New Algicide, Thiazolidinedione Derivative (TD53) to Marine Ecosystem

“…이러한 유해 조류를 조절하기 위한 방법으로 황산구리 [2], 과산화수소 [3], 황토 [4,5]나 점토 [6], 바이러스 생물학 제제 [7]와 박테리 아 [8]를 이용하는 방법에 관한 많은 연구가 수행되었다. 그러 나 이러한 방법들은 잠재적으로 심각한 환경적인 문제를 초래 할 수 있다.…”