Isolation, identification and characterization of bacterial flora from the respiratory tract of apparently healthy sheep

Sarker, Chandan Kumar; Hossen, A; Yousuf, Md. Abu; Uddin, Md. Ala; Akter, Most Sumona; Rahman, M. Mostafizur; Rahman, Bahanur

doi:10.3329/ajmbr.v1i3.26496

Cited by 4 publications

(4 citation statements)

References 3 publications

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“…The results of Gram's staining of E. coli in this study are similar with the findings of Thomas et al (2005); similarly, the results for Staphylococcus spp. are same as reported by Brooks et al (2002) and Sarker (2009), the results for Salmonella spp. are same as of Cheesbrough (1985) and Sannat et al (2017), and results for Bacillus spp.…”

Section: Morphological Characterization Of the Isolated Bacteriasupporting

confidence: 91%

Bacterial Flora Isolated From Dried Fishes Sold at Retail Markets Within Dhaka City Corporation of Bangladesh

Hassan

Poppy

Ahamed

et al. 2021

Res. Agric. Livest. Fish.

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Along with other contaminants, bacterial contamination in the dried fishes is a common issue which severely affects the quality of cured fishes. Hence, the present study was aimed to isolate and identify different bacterial flora contaminating different dried fishes, sold at different retail markets within Dhaka Metropolitan city, Dhaka, Bangladesh. A total of 25 different dried fishes were collected from Dhaka North city corporation area (Town Hall kacha bazar, Krishi market, and Mohammadpur kacha bazar) and Dhaka South city corporation area (New market kacha bazar, and Jatrabari chowrasta bazar). Each sample was divided into three regions (head, body, and tail) and bacterial isolation and identification was done by studying their cultural, staining and biochemical properties. Of the 25 dried fishes, Escherichia coli, Bacillus spp., Staphylococcus spp., Salmonella spp. and unidentified bacteria isolation rates were 44%, 56%, 80%, 48%, and 24%, respectively. The presence of E. coli and Salmonella spp. in various dried fish samples could be considered as indication of lack of hygienic condition during dried fish processing. Confirmation through molecular detection methods, pathogenicity, and antibiogram of the isolated bacteria could be included for future study. Res. Agric., Livest. Fish.8(1): 109-115, April 2021

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Section: Morphological Characterization Of the Isolated Bacteriasupporting

confidence: 91%

Bacterial Flora Isolated From Dried Fishes Sold at Retail Markets Within Dhaka City Corporation of Bangladesh

Hassan

Poppy

Ahamed

et al. 2021

Res. Agric. Livest. Fish.

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“…These clinical manifestations resembled to those described by [30]. The identi cation of M. haemolytica with biochemical methods is often challenging in some conditions due to antibiotic treatment, vaccination, transportation and storages [31]. Of the 213 cultures two (2) nasal swabs were tentatively identi ed as M.haemolytica.…”

Section: Discussionsupporting

confidence: 68%

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

Umer,

Bulto,

Bekele

et al. 2023

Preprint

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Background:Mannhemia haemolytica is one of the most important bacteria among causative agent of pneumonic pasteurellosis in small ruminants throughout worldwide and it is also economically devastating pathogen in Ethiopia. Methods: A cross sectional study was carried out from November 2021 to May 2022 with the aim to identify Mannheimia haemolytica from sheep and goat in Sebeta and Holeta town, Oromia special zone, Ethiopia. A total of 235 samples (213 nasal swabs and 22 whole blood) were collected. Sheep and goat with clinical signs suggestive of pneumonic pasteurollosis were purposively sampling. Bacterial identification was conducted using biochemical, Biolog, Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI TOF MS) and Real time PCR detection. Moreover, antimicrobials susceptibility test was also conducted on the identified bacterial isolates using disc diffusion method. Results: The result showed that from a total of 235 samples, only two nasal swab samples were positive for M. haemolytica (0.85%). The two isolates were confirmed by all the tests and similar result was obtained by; biochemical, Biolog, MALDI TOF MS and real time PCR. Up on antimicrobial susceptibility testing, the two isolates were resistant to Streptomycin, Erythromycin and Clindamycin whereas they were susceptible to Tetracycline, Chloramphenicol, Trimethoprim/sulfonamides and Penicillin. Generally, this study revealed that M. haemolytica is among the causative agent of pneumonic pasteurellosis in sheep and goat in the study area. Although, the other remaining bacteria responsible for the disease. Conclusion: The research suggests that a combination of diagnostic methods such as MALDI TOF MS, Biolog, and real-time PCR should be used, as well as for a more in-depth investigation to identify the strain or serotype of M. haemolytica using advanced molecular sequencing and also analysis of the remaining causal agent from various species and locations in countries are significant to address the present vaccination and antibiotic resistance issues.

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“…[177][178][179] Bacteria of the respiratory tract of sheep have also been isolated and identified. 180 Among the virus diseases, the genetic characterization of PPR in goats, 181,182 isolation and characterization of contagious ecthyma (CE) in both the goats and sheep 183 and epidemiology and phylogenetic studies on CE in goats 184 have been reported from Bangladesh. [181][182][183][184] Molecular detection of cellular prion protein in goats have also been reported.…”

Section: Microbiological Researchmentioning

confidence: 99%

A systematic review of pre-clinical and clinical research reports on small ruminants published during the last six decades in the then East Pakistan and in Bangladesh

Samad¹

2019

JVMOHR

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Background: A dozen of veterinary academic institutions offer degree courses with pre-clinical and clinical studies and conduct research works but their activities are not coordinated and unified in Bangladesh. An attempt has been made to review the pre-clinical and clinical research reports in small ruminants which would help the concerned academicians, research scientists and research funded organizations to update their knowledge of specialization and use this reference as a starting point for formulation of the future academic and research guidelines. Objectives: The main objectives are to provide an integrated, systematic overview of all the available published research reports on the pre-clinical and clinical aspects of small ruminants to update knowledge and to identify research insights, existing gaps and future research directions. Materials and Methods: The available research reports on pre-clinical and clinical aspects of small ruminants published mainly in journals during the last six decades in Bangladesh from 1966 to 2019 were reviewed. Data obtained were analyzed statistically based on pre-clinical and clinical subjects, host species, age, breeds, clinical prevalence and decade-wise to assess the rate of publication. Results: Of the 665 selected articles, 621 research papers published on small ruminants during the last six decades from Bangladesh were systematically reviewed, of which 340 (54.75%) articles reported as pre-clinical and 281 (45.25%) on clinical aspects with significantly higher rates in goats (n = 486; 78.26%) than sheep (n = 135; 21.74%). Out of 126 recorded clinical diseases and disorders in small ruminants, 125 (99.21%) reported in goats and 44 (34.92%) in sheep, with 43 (34.13%) common in both goats and sheep but only pregnancy toxaemia (0.79%) reported in sheep. The influence of age and breeds on the prevalence of clinical diseases and disorders associated with their morbidity and mortality are described and discussed. Conclusions: This unified review article would serve as a guide for the veterinary medical academicians, research scientists and organizations for future studies and research directions. The disease profiles and their morbidity and mortality status in small ruminants might help the practicing veterinarians, government, human health care providers and neighboring countries who may need to take action.

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Isolation, identification and characterization of bacterial flora from the respiratory tract of apparently healthy sheep

Cited by 4 publications

References 3 publications

Bacterial Flora Isolated From Dried Fishes Sold at Retail Markets Within Dhaka City Corporation of Bangladesh

Bacterial Flora Isolated From Dried Fishes Sold at Retail Markets Within Dhaka City Corporation of Bangladesh

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

A systematic review of pre-clinical and clinical research reports on small ruminants published during the last six decades in the then East Pakistan and in Bangladesh

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