Isolation, identification and characterization of fluoride resistant bacteria: Possible role in bioremediation

Chouhan, Swapnila; Tuteja, Urmil; Flora, S.J.S.

doi:10.1134/s0003683812010036

Cited by 40 publications

(27 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acinetobacter strains have been explored for degradation of persistent organic pollutants (POPs) like atrazine (Cail et al 2003). In 2012, five fluoride-resistant strains were isolated and a maximum fluoride removal of 22.1 % was achieved by Pseudomonas aerugenosa (Chouhan et al 2012). Table 3 gives a comparative study of fluoride removal by processes other than bioremediation.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a fluoride-resistant bacterium Acinetobacter sp. RH5 towards assessment of its water defluoridation capability

et al. 2015

View full text Add to dashboard Cite

The present study investigates the defluoridation capability of fluoride-resistant bacteria from contaminated groundwater collected from Asanjola and Madhabpur, West Bengal, India. Seven strains of fluorideresistant bacteria were isolated employing culture media containing 10-250 mg/L of fluoride to evaluate their ability in reducing fluoride concentration in water. Five isolates exhibited significant amount of reduction in fluoride. Isolate RH5 achieved a maximum fluoride removal of 25.7 % from the media at 30°C and pH 7 after 8 days of incubation. Based on morphological, physiological characteristics and analysis of 16S rDNA gene sequence, isolate RH5 was identified as Acinetobacter sp. RH5. Growth of RH5 was analysed at a diverse pH range, and it could thrive at pH 5-10. The present investigation revealed that the selective pressure of fluoride results in growth of fluoride-resistant bacteria capable of secreting high-affinity anion-binding compounds. This bacterium played a dominant bioremediative role by concentrating the anions so that they become less available. Hence, the fluoride-resistant bacteria, Acinetobacter sp. RH5, could be used as a promising strain for application in water defluoridation from contaminated sites.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of a fluoride-resistant bacterium Acinetobacter sp. RH5 towards assessment of its water defluoridation capability

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The main advantage of this method is that it can provide information on the dynamics of demineralization and remineralization of the surface and subsurface of the hard tissues of the tooth. It can be used to study the process of demineralization and remineralization of dental caries, as well as the mechanism of action of fluoride in remineralization (Chouhan, Tuteja, & Flora, 2011). Although the chemical caries method has many advantages, it is also associated with a shortcoming namely, insufficient simulation of caries biology.…”

Section: Discussionmentioning

confidence: 99%

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

et al. 2020

View full text Add to dashboard Cite

With the gradual discovery of functional domains in natural proteins, several biologically inspired peptides have been designed for use as biomaterials for hard tissue regeneration and repair. In this study, we designed a tuftelin-derived peptide (TDP) and tested its effects on hydroxyapatite crystallization and remineralization of initial enamel carious lesions in vitro. Using circular dichroism spectroscopy, we found that TDP contained 36.1% β-sheets and β-turns, which could be influenced by calcium ions. We verified the ability of TDP to crystallize hydroxyapatite using transmission electron microscopy and its ability to bind to the enamel surface and hydroxyapatite using confocal laser scanning microscopy and Langmuir adsorption isotherms (K = 881.56, N = 1.41 × 10 −5). Artificial enamel lesions were generated on human enamel blocks and subjected to a 12-day pH cycling model and were treated with 25 μM TDP, 1 g/L sodium fluoride (NaF), or deionized water. We analyzed the results of remineralization by surface microhardness testing, polarized light microscopy, and transverse microradiography. The TDP group showed significantly higher surface microhardness recovery (49.21 ± 1.66%), shallower lesions (34.89 ± 4.05 μm), and less mineral loss (871.33 ± 81.49 vol%•μm) after pH cycling than the deionized water group (p < .05). There were no significant differences between the TDP and NaF groups. Our experiment indicated that TDP could regulate hydroxyapatite crystallization and promote remineralization of enamel caries in vitro.

show abstract

“…Sphingomonas metabolizes 3-fluorobenzoate by dioxygenation, yielding 3-and 4-fluorocatechol which after intradiol cleavage form 2-and 3-fluoromuconic acid (Boersma et al 2004). Chouhan et al (2012) isolated fluoride-resistant Pseudomonas aerugenosa. Also, Mukherjee et al (2015) isolated Acinetobacter RH5 which showed 25.7% bioremediation.…”

Section: Microbial Degradation Of Fluoride Compoundsmentioning

confidence: 99%

A reappraisal on biodegradation of fluoride compounds: role of microbes

Singh

Gothalwal

2017

Water & Environment J

View full text Add to dashboard Cite

Fluorine is a highly reactive gas, rarely occurs in nature, in elemental form. Fluorosis is an excruciating and crippling disease caused by fluoride intake. Microbial degradation is cost‐effective and eco‐friendly method of removing fluoride compounds. The enzymes and genes involved in the fluoride‐degrading pathway have been identified and characterised. The in‐silico studies on degradative enzymes and their molecular interaction can be more advantageous in understanding fluoride toxicity and its degradative efficiency. Microbes exhibiting fluoride degradative potential or fluoride adsorption potential can be used for bioremediation of fluoride‐contaminated water. Large population is affected by fluorosis; both the welfare state and researchers are required to address the problem. The microbial degradation of fluoride provides sustainable, cost‐effective and ecofriendly technology. In the present review, an attempt has been made to compile all the available literature related to biodegradation of fluoride compounds.

show abstract

Isolation, identification and characterization of fluoride resistant bacteria: Possible role in bioremediation

Cited by 40 publications

References 28 publications

Characterization of a fluoride-resistant bacterium Acinetobacter sp. RH5 towards assessment of its water defluoridation capability

Characterization of a fluoride-resistant bacterium Acinetobacter sp. RH5 towards assessment of its water defluoridation capability

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

A reappraisal on biodegradation of fluoride compounds: role of microbes

Contact Info

Product

Resources

About