Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3

Luo, Keming; Wang, Yunsheng; Zeng, Aiping; Zhou, Xiaomao; Luo, Feng; Bai, Lianyang

doi:10.1371/journal.pone.0108012

Cited by 29 publications

(19 citation statements)

References 12 publications

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“…Hatched boxes represent the transmembrane domains, while other domains are shown in gray; CACHE domains are predicted to have a role in small-molecule recognition; HAMP domains are composed of an α-helix forming a coiled-coil frequently found in signaling proteins, and methyl-accepting transducer domains are the signaling domains of chemoreceptors. On the right is a depiction of the full-length conservation of each chemoreceptor in different plant-colonizing Bacillus strains, namely, B. subtilis UD1022 (40), B. amyloliquefaciens GB03 and FZB42 (41, 42), B. methylotrophicus FKM10 (43), B. pumilus INR7 and WP8 (44, 45), and B. megaterium Q3 (46). …”

Section: Resultsmentioning

confidence: 99%

“…Since B. subtilis strain 3610 was shown to strongly colonize the root, we wanted to examine the degree of conservation of these chemoreceptors in other root-colonizing Bacillus strains, specifically B. subtilis UD1022 (40), B. amyloliquefaciens GB03 and FZB42 (41, 42), B. methylotrophicus FKM10 (43), B. pumilus INR7 and WP8 (44, 45), and B. megaterium Q3 (46). The published genomes of these bacterial strains were searched for homologs of all 10 B. subtilis chemoreceptors.…”

Section: Resultsmentioning

confidence: 99%

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Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Allard-Massicotte

Tessier

Lécuyer

et al. 2016

mBio

212

179

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Colonization of plant roots by Bacillus subtilis is mutually beneficial to plants and bacteria. Plants can secrete up to 30% of their fixed carbon via root exudates, thereby feeding the bacteria, and in return the associated B. subtilis bacteria provide the plant with many growth-promoting traits. Formation of a biofilm on the root by matrix-producing B. subtilis is a well-established requirement for long-term colonization. However, we observed that cells start forming a biofilm only several hours after motile cells first settle on the plant. We also found that intact chemotaxis machinery is required for early root colonization by B. subtilis and for plant protection. Arabidopsis thaliana root exudates attract B. subtilis in vitro, an activity mediated by the two characterized chemoreceptors, McpB and McpC, as well as by the orphan receptor TlpC. Nonetheless, bacteria lacking these chemoreceptors are still able to colonize the root, suggesting that other chemoreceptors might also play a role in this process. These observations suggest that A. thaliana actively recruits B. subtilis through root-secreted molecules, and our results stress the important roles of B. subtilis chemoreceptors for efficient colonization of plants in natural environments. These results demonstrate a remarkable strategy adapted by beneficial rhizobacteria to utilize carbon-rich root exudates, which may facilitate rhizobacterial colonization and a mutualistic association with the host.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Allard-Massicotte

Tessier

Lécuyer

et al. 2016

mBio

212

179

View full text Add to dashboard Cite

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“…When viewed under the scanning electron microscope (SEM), strain CTBmeg1 appeared to have a bacilli-like shape with width and length between 745 ± 69.5 nm and 1.96 ± 32.7 lm, respectively (Figure 2(d)). The biochemical properties of strain CTBmeg1 were closely affiliated with those of Bacillus megaterium strains YJB3 [41], WS24 [42], G3 [43] and BJC3.1 [44]. The overall properties of strain CTBmeg1 bacterium is tabulated in Table 1.…”

Section: Characterization Of Strain Ctbmeg1mentioning

confidence: 99%

Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds

Akcay

Kaya

2019

Biotechnology & Biotechnological Equipment

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Halogenated compounds pose long-term potential risks to the well-being of humans due to their recalcitrance and persistent toxicity. The quest for microorganisms capable of degrading such perilous substances merits urgent consideration. In this study, a new dehalogenase-producing bacterium was isolated from a hypersaline environment (TuzG€ ol€ u Lake, Turkey), and identified as Bacillus megaterium strain CTBmeg1 (Accession number MK128900). Under culture conditions (pH 8.0, NaCl 20%, 30 C, 200 rpm, 9 days), the B. megaterium strain CTBmeg1 showed an optimum growth on 10 mmol/L of 2,2-dichloropropionic acid with a doubling time of 26.41 h. Furthermore, the presence of a putative halotolerant dehalogenase gene (dehCTBmeg1) of B. megaterium strain CTBmeg1 was detected and amplified via PCR technique. Bio-prospecting for microorganisms in a highly saline environment capable of utilizing halogenated compounds as the sole carbon may prove to be a practical and safer means for bioremediation of contaminated coastal areas, an increasingly common place predicament faced by many nations.

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“…A study isolated endophytic bacteria Pseudomonas oryzihabitans and Burkholderia gladioliwhich from soybean grown in soil treated with glyphosate herbicide [ 22 ]. Recently, an endophytic quinclorac-degrading bacterium Bacillus megaterium Q3 was isolated from the root of tobacco grown in quinclorac-contaminated soil [ 23 ]. Up to now, it has not been reported endophytic microorganisms able to degrade diuron.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils

Wang

Feng

et al. 2017

PLoS ONE

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A diuron-degrading endophyte DP8-1 was isolated from sugarcane root grown in diuron-treated soil in the present study. The endophyte was identified as Neurospora intermedia based on the morphological characteristics and sequence analysis. The fermentation parameters including temperature, pH, inoculation size, carbon source, and initial diuron concentration were also investigated for the optimization of degradation efficiency. The results indicated that strain DP8-1 was capable of degrading up to 99% diuron within 3 days under the optimal degrading condition. The study of degradation spectrum indicated that strain DP8-1 could also degrade and utilize fenuron, monuron, metobromuron, isoproturon, chlorbromuron, linuron, and chlortoluron as substrate for strain growth. On basis of liquid chromatography-mass spectrometry analysis for the products of the degradation of diuron, strain DP8-1 metabolized diuron to produce N-(3,4-dichlorophenyl)-urea and N-(3,4-dichlorophenyl)-N-methylurea through sequential N-dealkylations. In a soil bioaugmentation experiment, the inoculation of strain DP8-1 into diuron-treated soil effectively enhanced the disappearance rate of diuron.

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Isolation, Identification and Characteristics of an Endophytic Quinclorac Degrading Bacterium Bacillus megaterium Q3

Cited by 29 publications

References 12 publications

Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds

Biodegradation of diuron by an endophytic fungus Neurospora intermedia DP8-1 isolated from sugarcane and its potential for remediating diuron-contaminated soils

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