Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico

Morales-Estrada, Aurea Itzel; Hernández‐Castro, Rigoberto; López-Merino, Ahidé; Singh-Bedi, J; Contreras-Rodríguez, Araceli

doi:10.4067/s0301-732x2016000200014

Cited by 10 publications

(5 citation statements)

References 10 publications

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“…The resistance rate of the Brucella strains to trimethoprim/sulfamethoxazole determined in this study (1.6%) was notably lower than reported in Ulanqab in south-central Inner Mongolia (7.06%) [23], Xing'anmeng in Inner Mongolia (20%) [8, 9,24], while the rate of resistance to azithromycin was similar to that in Ulanqab and Xing'anmeng (100%), suggesting that Brucella could gradually become resistant to azithromycin in the near future [8,9]. In contrast to the results of the present study, isolates collected in Mexico in 2016, Kazakhstan in 2017, and Egypt in 2019 were resistant to cipro oxacin, streptomycin, rifampicin, and levo oxacin [10,24,25].…”

Section: Discussioncontrasting

confidence: 77%

Molecular Characterization and Antimicrobial Susceptibility of Human Brucella in Northeast China

Wang

et al. 2022

Preprint

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Background Brucella is a widespread and highly pathogenic bacteria that poses serious threats to public health and animal husbandry. The aim of this study was to identify the characteristics of Brucella spp. in Northeast China, to determine their antimicrobial resistance, and to preliminarily analyze the resistance mechanism. Methods In this study, 61 Brucella isolates were identified by abortus-melitensis-ovis-suis polymerase chain reaction, and epidemic potential was clarified by multi-locus sequence analysis. Whole-genome sequencing (WGS) was performed and the antibiotic susceptibility of the Brucella strains against 13 antibiotics was detected with the use of E-test strips. Results The results showed that all of the isolates were Brucella melitensis ST8, group CC4 with little genetic variation and obvious geographical characteristics. All 61 Brucella isolates were sensitive to doxycycline, tetracycline, minocycline, levofloxacin, ciprofloxacin, gentamicin, and streptomycin, while 24.6%, 86.9%, 65.6%, 27.9%, 3.3%, and 1.6% were resistant to rifampin, azithromycin, cefepime, cefoperazone/sulbactam, cefotaxime, and meperidine/sulfamethoxazole, respectively. This is the first report of cephalosporin-resistant B. melitensis in China. The WGS results indicated that about 60% of the antibiotic resistance genes were associated with efflux pumps (mainly the resistance nodulation division family). Conclusions Brucellosis is usually treated with antibiotics for several months, which can easily lead to the emergence of antibiotic resistance. To ensure the effectiveness and safety of antibiotics for treatment of brucellosis, continuous surveillance of antibiotic susceptibility is especially important.

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“…The resistance rate of the Brucella strains to trimethoprim/sulfamethoxazole determined in this study (1.6%) was notably lower than reported in Ulanqab in south-central Inner Mongolia (7.06%) [23], Xing'anmeng in Inner Mongolia (20%) [8, 9,24], while the rate of resistance to azithromycin was similar to that in Ulanqab and Xing'anmeng (100%), suggesting that Brucella could gradually become resistant to azithromycin in the near future [8,9]. In contrast to the results of the present study, isolates collected in Mexico in 2016, Kazakhstan in 2017, and Egypt in 2019 were resistant to cipro oxacin, streptomycin, rifampicin, and levo oxacin [10,24,25].…”

Section: Discussioncontrasting

confidence: 77%

Molecular Characterization and Antimicrobial Susceptibility of Human Brucella in Northeast China

Wang

et al. 2022

Preprint

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“…The resistance rate of the Brucella strains to trimethoprim/ sulfamethoxazole determined in this study (1.6%) was notably lower than reported in Ulanqab in south-central Inner Mongolia (7.06%), Xing'anmeng in Inner Mongolia (20%) (Morales-Estrada et al, 2016;Liu et al, 2018;Yuan et al, 2020), while the rate of resistance to azithromycin was similar to that in Ulanqab and Xing'anmeng (100%), suggesting that Brucella could gradually become resistant to azithromycin in the near future (Liu et al, 2018;Yuan et al, 2020). In contrast to the results of the present study, isolates collected in Mexico in 2016, Kazakhstan in 2017, and Egypt in 2019 were resistant to ciprofloxacin, streptomycin, rifampicin, and levofloxacin (Morales-Estrada et al, 2016;Shevtsov et al, 2017;Khan et al, 2019). Notably, this study is the first to report cephalosporin-resistant Brucella isolates in China and relatively high resistance to cefepime and cefoperazone/sulbactam.…”

Section: Discussioncontrasting

confidence: 74%

Molecular characterization and antimicrobial susceptibility of human Brucella in Northeast China

Wang

et al. 2023

Front. Microbiol.

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IntroductionNortheast China has always been an area with severe brucellosis prevalence. This study will identify Brucella in Northeast China and test its resistance to antibiotics, in order to clarify its resistance mechanism. Brucella is a widespread and highly pathogenic bacteria that poses serious threats to public health and animal husbandry.MethodsIn this study, 61 Brucella isolates were identified by abortus-melitensis-ovis-suis polymerase chain reaction (AMOS-PCR) for biotypes and epidemic potential was clarified by multi-locus sequence analysis. Whole-genome sequencing (WGS) was performed and the antibiotic susceptibility of the Brucella strains against 13 antibiotics was detected with the use of E-test strips.ResultsThe results showed that all of the isolates were Brucella melitensis ST8, group CC4 with little genetic variation and obvious geographical characteristics. All 61 Brucella isolates were sensitive to doxycycline, tetracycline, minocycline, levofloxacin, ciprofloxacin, gentamicin, and streptomycin, while 24.6%, 86.9%, 65.6%, 27.9%, 3.3%, and 1.6% were resistant to rifampin, azithromycin, cefepime, cefoperazone/sulbactam, cefotaxime, and meperidine/sulfamethoxazole, respectively. This is the first report of cephalosporin-resistant B. melitensis in China. The WGS results indicated that about 60% of the antibiotic resistance genes were associated with efflux pumps (mainly the resistance nodulation division family).DiscussionBrucellosis is usually treated with antibiotics for several months, which can easily lead to the emergence of antibiotic resistance. To ensure the effectiveness and safety of antibiotics for treatment of brucellosis, continuous surveillance of antibiotic susceptibility is especially important.

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“…Although the Brucella species are generally considered to be host-specific, it is not very uncommon (unlikely) that cross-species infections can occur in animals under certain circumstances like sharing the same stables, pasture, and facilities, or due to the close proximity of the farms and the uncontrolled movement of infected animals (Morales-Estrada et al 2012;Morales-Estrada et al 2016;Kolo et al 2019). Kolo et al (2019) Cattle can also be infected with B. suis and B. melitensis when they graze together with infected pigs, goats or sheep.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterisation of Brucella melitensis by bacteriological and molecular methods from livestock in North Cyprus

Demirpence¹,

Saytekin²,

Sareyyüpoğlu³

et al. 2022

Vet. Med. - Czech

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In this study, the isolation, biotyping and molecular characterisation of Brucella melitensis from cattle, sheep and goats in North Cyprus are reported on. A total of 319 raw milk samples obtained from seropositive dairy livestock (190 cattle, 74 sheep and 55 goats) and tissue samples including the liver, spleen and abomasal contents obtained from 32 aborted foetal samples (5 cattle, 18 sheep and 9 goats) were analysed for the presence and characterisation of the agent. B. melitensis was isolated and identified from 90 out of 319 (28.2%) milk and 19 out of 32 (59.4%) foetal samples by conventional bacteriological methods. Identification of all 109 isolates was confirmed by using real-time PCR with genus and species-specific primers. Following the preliminary identification, 27 selected isolates representing various counties and herds were further analysed by conventional methods. Twenty (74.1%) isolates were identified as B. melitensis biovar 1 and seven (25.9%) were identified as B. melitensis biovar 3. The Bruce-ladder multiplex PCR assay revealed that all the isolates were field strains. The results of the present study confirmed the presence of B. melitensis in livestock including the cattle population in North Cyprus. Even though the majority of the samples came from seropositive cattle, Brucella abortus was not isolated in the study. The results also revealed the potential public health risk of brucellosis in livestock emphasising the need of implementing strict control and eradication strategies against the disease in animal populations in order to protect human health.

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Isolation, identification, and antimicrobial susceptibility of Brucella spp. cultured from cows and goats manure in Mexico

Cited by 10 publications

References 10 publications

Molecular Characterization and Antimicrobial Susceptibility of Human Brucella in Northeast China

Molecular Characterization and Antimicrobial Susceptibility of Human Brucella in Northeast China

Molecular characterization and antimicrobial susceptibility of human Brucella in Northeast China

Isolation and characterisation of Brucella melitensis by bacteriological and molecular methods from livestock in North Cyprus

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