Isolation, identification and antibiogram profiles of enterovirulent Escherichia coli from diarrhoeic goat in some selected areas of Rangpur district of Bangladesh

Abstract

Enterovirulent Escherichia coli remain as an important etiological agent of goat diarrhoea inBangladesh. The present study was designed with a view to isolate and identifies E. coli from field cases. For this purpose, a total of 135 faecal samples (85 from diarrhoeic and 50 from apparently healthy goat) were collected during the period from January 2012 to July 2012 from different areas in Rangpur District. It was found that the prevalence of E. coli was higher (18.82 %) in diarrhoeic goats while it was lower (14.00 %) in non diarrhoeic goats. Age wise distribution of E. coli isolates were 26.42% in day old to 1 year, 10.53% in 1-2 years and 11.36% in above 2 years age of goat respectively. All the isolates of E. coli revealed greenish black colony with metallic sheen in Eosine methylene blue agar, bright pink color smooth transparent colony in MacConkey agar and slight pinkish smooth colony in Salmonella-Shigella agar. Gram stain and hanging drop techniques were performed with the cultured bacteria. Biochemical properties of the isolates were studied, and antibiotic sensitivity test was done by agar disk diffusion method. In Gram stain, the organisms revealed Gram negative, small rod shaped, occurs singly or paired. Biochemically, all of the isolates showed fermentation of dextrose, sucrose and maltose with the production of acid and gas, negative result to Voges-Proskauer test, positive result to Methylred test and differential result to Indol test. All the isolates of E. coli were highly sensitive to ciprofloxacin and gentamicin while moderately sensitive to colistin, livofloxacin and azithromycin and less sensitive to ceftraexon and tetracyclin and resistant to amoxycillin, ampicillin, erythromycin, and neomycin. Therefore, ciprofloxacin and gentamicin may be the antibiotics of first choice, and colistin, livofloxacin and azithromycin may be the second choice among the test antibiotics for the treatment of illness caused by these bacteria.

A systematic review of pre-clinical and clinical research reports on small ruminants published during the last six decades in the then East Pakistan and in Bangladesh

“…The Escherichia coli and Campylobacter jejuni of goats and E. coli and Salmonellae of sheep have been isolated and characterized. [158][159][160][161][162][163][164][165][166][167][168][169]171 Sero-molecular diagnosis of brucellosis in goats and sheep have been reported. 170 Bacterial pathogens have also been isolated and identified in samples of upper respiratory tract (pneumonia), buccal and intestinal samples and pneumo-enteritis affected goats and samples of slaughtered yards and meat stalls.…”

Prevalence of Potential Zoonotic Bacterial Pathogens Isolated From Household Pet Birds and Their Antimicrobial Profile in Northern Bangladesh

“…Recent studies reported 50-55% prevalence of E. coli in cloacal and oral swab of pigeons, 43.7% prevalence in feces of ornamental bird (Karim et al 2020;Akbari and Asapour 2022). Other studies recorded 60.5% prevalence of E. coli in chicken meat, 78.8% prevalence in poultry, 66.6% prevalence in duck, 14.4-24.5% prevalence in psittacine bird, 32.8-64.4% prevalence in parrot, 16.9% prevalence in budgerigar, 37.2-40% prevalence in quail, 10-31.2% prevalence in wild bird, 18.82% prevalence in diarrheic goat and 65% prevalence in animal feed (Hedawy and El-Shorbagy 2007;Akhter et al 2010;Dey et al 2013;Ghazi et al 2014;Youssef and Mansour 2014;Sanches et al 2017;Sultana et al 2017;Kamal et al 2018;Habib et al 2021). Our study revealed the presence of Salmonella spp.…”

“…A total of 240 samples were collected during July 2016 to June 2017 from various sources, cloacal swab (n=80), oral swab (n=80) and feces (n=80) in different locations across the district. All these samples were collected from pigeon (n=40), 20 parrot (n=20), budgerigar (n=12) and quail (n=8) were maintaining aseptic techniques (Kamal et al 2018;Haque et al 2022). Briefly the samples were collected by sterile cotton buds and took into sterile tube containing 1% peptone water.…”

Recent trends and scenario of antibiotic use in veterinary practices for livestock production in Bangladesh: a review

“…The improper antimicrobial drug used for humans is diffusive in developing countries and is a significant contributor to growing the public health threat of AMR-resistant bacteria (Roess et al, 2013). In recent years, the indiscriminate use of antibiotics in animals and poultry is thought to be an important factor to develop of AMR Al-Salauddin et al, 2015;Sohidullah et al, 2016;Rahman et al, 2016;Talukder and Ahmed, 2016;Roy et al, 2017;Jahan et al, 2017;Kabir et al, 2018a;Kabir et al, 2018b;Kamal et al, 2018;Alam et al, 2019;Rumi et al, 2019;Saif et al, 2019;Masud et al, 2020;Alam et al, 2020;Sarker et al, 2020;Mridha et al, 2020;Matubber et al, 2021;Uddin et al, 2021;Tresha et al, 2021;Eashmen et al, 2021;Haque et al, 2021;Hosain et al, 2021;Arif et al, 2022;Hoque et al, 2022). In Bangladesh, the most common reason for choosing an antimicrobial is personal experience and perception (68%), rather than the cultural sensitivity test which may be due to a lack of vet diagnostic facilities and the unwillingness of the veterinary personnel (Pokharel et al, 2020).…”