Isolation, genomic and biological characterizations of a rhabdovirus from mandarin fish (Siniperca chuatsi)

Recovery of Siniperca chuatsi rhabdovirus from cloned cDNA

“…A similar phenomenon occurred in the rescue of SHRV in Novirhabdovirus (Johnson et al, 2000). The minigenome assay of SCRV showed that the transfection with the T7 expression plasmid was not able to showed that SCRV was able to infect BSRT7 cells and cause significant CPE at 28°C indicating that SCRV could replicate well in BSRT7 cells (Liu et al, 2023). The mammalian cell line BHK-21 was also successfully used for the rescue of IHNV at 15°C (Zhao et al, 2019).…”

“…BSRT7 is one kind of genetically engineered mammalian cell (BHK‐21 cells) that stably expresses T7 RNA polymerase. Although the normal culture temperature for BSRT7 is 37°C, our study showed that SCRV was able to infect BSRT7 cells and cause significant CPE at 28°C indicating that SCRV could replicate well in BSRT7 cells (Liu et al, 2023). The mammalian cell line BHK‐21 was also successfully used for the rescue of IHNV at 15°C (Zhao et al, 2019).…”

“…The fish‐derived cells of EPC are very sensitive to SCRV. SCRV S3 can replicate in EPC cells and cause significant cytopathic effects (CPE) in 2 days (Liu et al, 2023). However, the recovery of recombinant SCRV by this method failed after several attempts.…”

“…Because mandarin fish is a carnivorous fish, it is difficult to cultivate in the laboratory. Our recent study indicated that red zebrafish can be used as an infection model for SCRV (Liu et al, 2023). We employed zebrafish for virus challenge experiments.…”

“…BSRT7 cells (baby hamster kidney cells expressing T7 RNA polymerase) were grown in Dulbecco's modified Eagle's medium (DMEM: HyClone; Thermo) supplemented with 10% (v/v) FBS. SCRV S3 strain was isolated from mandarin fish ( S. chuatsi ) in 2018 (Liu et al, 2023). The full genome sequence of S3 was deposited in the GenBank database (accession no.…”