Thermal and environmental stability of Siniperca chuatsi Rhabdovirus

Xu, Zhendong; Huang, Zhiyang; Zhong, Junyao; Zhu, Yinzhi; Liu, Xiaoyu; Wei, Yongwei

doi:10.1016/j.aquaculture.2023.739308

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…Additionally, UV radiation is another effective way to inactivate viruses in both laboratory and cultural settings. Our results showed that the CAPRV2023 isolate was highly sensitive to UV light and could be completely inactivated following irradiation for 45–60 s, which is in agreement with previous findings on SCRV and VHSV [39,43]. Currently, the possibility of the vertical transmission of CAPRV cannot be ruled out.…”

Section: Discussionsupporting

confidence: 91%

“…However, even when exposed to the highest recorded water temperature of 33°C in the South China Sea during summer, CAPRV can maintain its infectivity for up to 1–2 weeks. This duration is slightly shorter than that of the SCRV [39] but slightly longer than that of the VHSV [40]. Further, when exposed to CAPRV by immersion in seawater containing a pure culture of the virus, a high mortality rate in the infection group with characteristic clinical signs was observed, suggesting that the virus can be transmitted horizontally.…”

Section: Discussionmentioning

confidence: 99%

“…Thermal inactivation at 55°C for 30 min is a commonly used method for virus inactivation. The titer of the virus remained high when SCRV was inactivated at 55°C for 30 min [39]. The temperature and duration required to inactivate the NNV were relatively low [41].…”

Section: Discussionmentioning

confidence: 99%

“…reported in several studies [33,34]. Among these environmental factors, temperature is the key parameter for viral proliferation and pathogenicity because outbreaks of viral diseases in fish are typically temperature dependent [35][36][37][38][39][40][41][42][43][44][45]. For example, IHN outbreaks in salmonid fish usually occur at water temperatures of 8-14℃ and have not been observed above 15℃, whereas IHNV replicated most rapidly at 15℃ [35,36].…”

Section: Discussionmentioning

confidence: 99%

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Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Sun,

Huang,

Wang

et al. 2024

Preprint

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A severe epizootic outbreak occurred in a deep-water cultured golden pompano (Trachinotus ovatus) in Guangdong Province, China (August–November 2023); the cumulative mortality was 65–82%. The diseased fish showed clinical signs of lethargy, anorexia, whirling movements, and hemorrhage at the base of the fins and in the upper and lower jaws before mass mortality. A Rhabdovirus strain, CARPV2023, was successfully isolated using FHM cells. Cytopathic effects of the tissue filtrate of the diseased golden pompano on FHM and EPC cell monolayers were characterized by rounded cells, grape-like cluster formation, detachment, and lysis. Histopathology revealed congestion, hemorrhage, and necrosis in the spleen, liver, kidney, and intestinal tissues of naturally and experimentally infected golden pompanos. Electron microscopy showed that bullet-shaped viral particles 183.5–201.6-nm long and 57.3–82.7-nm wide budded from cell membrane or aggregated around the infected FHM cells. The virus remained stable for 7 days at 4–33 °C and grew optimally at 28 °C. Whole-genome sequencing and phylogenetic analysis based on the full genome further indicated that the CARPV2023 strain is a member of Carpione Rhabdovirus, which is related to Novirhabdovirus with unclassification. A specific nested reverse transcriptase-polymerase chain reaction confirmed the presence of CARPV in the affected golden pompano. Much higher mortality was observed in challenged golden pompanos than in the controls through intraperitoneal injection and immersion infection. In situ hybridization showed positive reactions in the fish tissues. This is the first study to report natural CARPV infections in marine fish in the world.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Sun,

Huang,

Wang

et al. 2024

Preprint

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“…Chinese rice-eld eel rhabdovirus (CrERV) shared the greatest similarity (94.5% identity) with SCRV (Liu et al, 2019a). SCRV can survive for months in liquids at moderate temperatures so that has caused high morbidity and mortality in sh (Xu et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic profiling of the immune response to Siniperca chuatsi rhabdovirus (SCRV) in E11 cells

Zhang,

Sun,

Tan

et al. 2024

Aquacult Int

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Siniperca chuatsi rhabdovirus (SCRV) has caused severe disease and mortality in commercial sh in China. To investigate the molecular relationships between pathogen and host, transcriptome analysis of SCRV-infected and non-infected E11 cells was obtained using the Illumina HiSeq X Ten sequencing system. There was 29,894 unigenes assembled and a total of 4137 differentially expressed genes (DEGs), consisting of 2189 up-regulated and 1948 down-regulated unigenes were detected between SCRV-infected and non-infected E11 cells. To understand the immune response of the virus in the host, pathway function analysis indicates that SCRV infection affected 31 typical immune-related pathways, including MAPK signaling pathway, PI3K-Akt signaling pathway, Endocytosis and Apoptosis, suggesting that E11 cells resist SCRV invasion through these innate immune pathways. Overall, qRT-PCR results showed similar expression to Illumina HiSeq, with reliable sequencing results. These results provided some vital clues which will be helpful to future study on SCRV-host interactions.

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Infection tracing and organ tropism of Siniperca chuatsi rhabdovirus expressing enhanced green fluorescent protein

Liu,

Huang,

Miao

et al. 2023

Aquaculture

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Thermal and environmental stability of Siniperca chuatsi Rhabdovirus

Cited by 5 publications

References 39 publications

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Mass mortality associated with Carpione Rhabdovirus in golden pompano (Trachinotus ovatus) in China: First report

Transcriptomic profiling of the immune response to Siniperca chuatsi rhabdovirus (SCRV) in E11 cells

Infection tracing and organ tropism of Siniperca chuatsi rhabdovirus expressing enhanced green fluorescent protein

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