Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview

Abstract

The aim of this review was to describe the current state-of-the-art regarding isolation, characterization and aging of adipose tissue-derived mesenchymal stem cells (ADSCs). Mesenchymal stem cells (MSCs) have recently received widespread attention because of their potential use in tissue-engineering applications. Various studies have indicated that MSCs with a fibroblast-like morphology migrate to the sites of injury and help to regenerate damaged tissue. Over the past few years, it has been recognized that fat is not only an energy supply, but also a rich source of multipotent stem cells that can be easily harvested, isolated and selected as compared with other tissues. ADSCs are particularly interesting because of their rapid proliferation and multidirectional differentiation potential.

Curcumin Affects Adipose Tissue-Derived Mesenchymal Stem Cell Aging Through TERT Gene Expression

“…The use of stem cells from different tissues for regenerative medicine applications has increased over the past few years [21][22][23][24]. Similar to normal somatic cells, adult stem cells are exposed to stressors during their life span, leading to an age-dependent decline in their number and function [25]. MSCs transplanted from the aged donors are less effective.…”

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways

“…A slight influence on the stem cells may cause unexpected consequences to its related adult cells [30]. MSCs are pluripotent cells and are characterized by their self-renewal abilities through mitotic cell division, as well as by their potential to differentiate into multiple types of lineages such as: chondrogenic, osteogenic, adipogenic, myogenic, neurogenic, and hepatogenic [31, 32]. Adipose tissue has become a rich source of MSCs, providing abundant and accessible source of ADSCs with minimal patient discomfort.…”

Adipose-derived stem cells: a review of osteogenesis differentiation

“…They secrete factors such as: TGF-β , 11,, inducible nitric oxide synthase (iNOS) and hemooxygenase (HO). MSCs can modulate immunological responses through T-cell-mediated (Takano et al 2014, Rahimzadeh et al 2014 They release also multiple angiogenic and growth factors: VEGF, HGH or IGH-1 (Fathi et al 2016, Soulnier et al 2010. It suggest that they are improving neovascularization and promote angiogenesis process (Abudusaimi et al 2011).…”

“…Procedure of liposuction can provide a lot of tissue and cells. Moreover isolation of stem cells from bone marrow is less effective and cells often are contaminate (Chen at al.2013, Fathi et al 2016, Dai et al 2016. Comparison of mesenchymal stem cells obtained from different tissues (fat tissue, bone marrow, placenta) showed that adipose stem cells did not differ morphologically from bone marrow cells.…”

“…Based on it human MSCs identified by adherence to plastic and expression of cell surface markers including CD90, CD73, CD105 and lack of expression of CD45, CD34, CD14 or CD11b, CD79a or CD19 and HLA-DR surface proteins. There also must differentiate to osteoblasts, adipocytes and chondroblasts in vitro condition (Dominici et al 2006, Fathi et al 2016, Bourin 2013)(Tab. 1).…”