Isolation, Characterization, Determination of Probiotic Properties of Lactic Acid Bacteria from Human Milk

Jayaram, Kavitha; Devasena, T

doi:10.9790/3008-0730107

Cited by 19 publications

(27 citation statements)

References 14 publications

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“…Probiotic strains can be isolated directly from natural fermented milk products or milk and can then be added as starter cultures for fermentation in products such as cheese, yogurt, and butter. Potential probiotics have been isolated from human sources from different parts of the human body, including the human feces of both healthy adults [ 51 ] and breast-fed infants [ 52 ], as well as human breast milk [ 53 , 54 ].…”

Section: Introductionmentioning

confidence: 99%

“…Probiotics isolated from human breast milk have usually been of the Lactobacillus genus [ 54 , 55 ], while probiotics from the feces of healthy human adults and breast-fed infants have been from at least two genera: Lactobacillus [ 55 – 57 ] and Bifidobacterium [ 52 ]. A few studies have reported probiotic Enterococcus faecalis found in human feces [ 58 ] .…”

Section: Introductionmentioning

confidence: 99%

“…L. salivarius has been found in human milk and infant feces in individuals of a mother-child pair [ 59 ]. L. rhamnosus and L. casei were isolated from human breast milk and showed resistance to low pH (pH 3), tolerance against a 0.3 % bile concentration and antimicrobial activity against Escherichia coli , Bacillus cereus and Staphylococcus aureus [ 54 ]. Another genus of probiotic, Pediococcus , which produces a bacteriocin-like substance, can be isolated from healthy human breast milk [ 60 ].…”

Section: Introductionmentioning

confidence: 99%

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Probiotic isolates from unconventional sources: a review

2016

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The use of probiotics for human and animal health is continuously increasing. The probiotics used in humans commonly come from dairy foods, whereas the sources of probiotics used in animals are often the animals’ own digestive tracts. Increasingly, probiotics from sources other than milk products are being selected for use in people who are lactose intolerant. These sources are non-dairy fermented foods and beverages, non-dairy and non-fermented foods such as fresh fruits and vegetables, feces of breast-fed infants and human breast milk. The probiotics that are used in both humans and animals are selected in stages; after the initial isolation of the appropriate culture medium, the probiotics must meet important qualifications, including being non-pathogenic acid and bile-tolerant strains that possess the ability to act against pathogens in the gastrointestinal tract and the safety-enhancing property of not being able to transfer any antibiotic resistance genes to other bacteria. The final stages of selection involve the accurate identification of the probiotic species.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Probiotic isolates from unconventional sources: a review

2016

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“…After incubation, plates were observed and zones of inhibitions were measured. 20,25,42,4 All the experiments in this study were carried out in triplicate and average values were taken under consideration, result and conclusion.…”

Section: Determination Of Antimicrobial Activitymentioning

confidence: 99%

“…Probiotic properties include antibacterial-antifungal activities, bile salt hydrolase activity, ability to survive at low pH and high salt concentration, adhesion assay, production of gamma amino butyric acid (GABA), β-galactosidase assay, hemolytic activity, mucin degradation etc. [1][2][3][4][5][6] Various methods are used for determination of these properties. Selection of method is highly dependent on the character and type of microorganism.…”

Section: Introductionmentioning

confidence: 99%

Determination of Probiotic Properties of L. fermentum MT308789 and L.oris MT308790 Isolated from Mother Breast Milk

Gondaliya¹,

Ramani

2022

Int J Life Sci Pharm Res

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Mother milk is a complete food for the child. It contains vital nutrients and biomolecules required for the growth and development of a child. It mainly contains carbohydrates, proteins, fats and minerals. Recently, it was also found that it contains certain different microorganisms which play a vital role in the body. Most of the microorganism of milk belongs to the Lactobacilli family. Knowing the importance of Lactobacilli in health, certain food industries have focused on these microorganisms to be used as a health supplement nowadays called probiotics. Here in this study, Lactobacilli were isolated from mother breast milk and their probiotic characteristics were studied to determine their potential for probiotic. Strains were isolated using MRS medium and analyzed for their biochemical properties, pH resistance, bile salt tolerance, NaCl concentration and antimicrobial activity. Results of the study have shown that out of two strains, one strain was L. fermentum MT308789 and other was L oris MT308790. Both the species have shown good resistance to acidic pH and bile salt. They were also having good antimicrobial activity. Based on the study it was found that both strains have potential to be used as probiotic.

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