Isolation, characterization and selection of indigenous Bradyrhizobium strains with outstanding symbiotic performance to increase soybean yields in Mozambique

Chibeba, Amaral Machaculeha; Kyei-Boahen, Stephen; Guimarães, María de Fátima; Nogueira, Marco Antônio; Hungría, Mariangela

doi:10.1016/j.agee.2017.06.017

Cited by 76 publications

(81 citation statements)

References 66 publications

(105 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Like most legumes, the N 2 -fixing trait of cowpea confers adaptation to low nutrient soils, with some genotypes deriving up to 96% of their nitrogen (N) requirements from symbiosis, which often leads to significant N contribution to cropping systems and grain yield increases [1] [4] [5]. However, due to variations in legume symbiotic performance resulting from genotypic differences and N 2 -fixing efficiency of the rhizobial symbionts, there is often the need to screen for improved N 2 fixation among legume germplasm [6] [7]. Of the many techniques used to estimate legume symbiotic performance, the 15 N natural abundance has so far been useful in quantifying N 2 fixation in field-grown legumes [8] [9].…”

Section: Introductionmentioning

confidence: 99%

Screening Cowpea (Vigna unguiculata (L.) Walp.) Genotypes for Enhanced N2 Fixation and Water Use Efficiency under Field Conditions in Ghana

Yahaya¹,

Denwar²,

Mohammed³

et al. 2019

AJPS

View full text Add to dashboard Cite

To explore the variations in symbiotic N 2 fixation and water use efficiency in cowpea, this study evaluated 25 USDA cowpea genotypes subjected to drought under field conditions at two locations (Kpachi and Woribogu) in the Northern region of Ghana. The 15 N and 13 C natural abundance techniques were respectively used to assess N 2 fixation and water use efficiency. The test genotypes elicited high symbiotic dependence in association with indigenous rhizobia, deriving between 55% and 98% of their N requirements from symbiosis. Consequently, the amounts of N-fixed by the genotypes showed remarkable variations, with values ranging from 37 kg•N-fixed•ha −1 to 337 kg•N-fixed•ha −1. Most genotypes elicited contrasting symbiotic performance between locations, a finding that highlights the effect of complex host/soil microbiome compatibility on the efficiency of the cowpea-rhizobia symbiosis. The test genotypes showed marked variations in water use efficiency, with most of the genotypes recording higher δ 13 C values when planted at Kpachi. Despite the high symbiotic dependence, the grain yield of the test cowpeas was low due to the imposed drought, and ranged from 56 kg/ha to 556 kg/ha at Kpachi, and 143 kg/ha to 748 kg/ha at Woribogu. The fact that some genotypes could grow and produce grain yields of 627-748 kg/ha under drought imposition is an important trait that could be tapped for further improvement of cowpea. These findings highlight the importance of the cowpea-rhizobia symbiosis and enhanced water relations in the crop's wider adaptation to adverse edaphoclimatic conditions.

show abstract

Section: Introductionmentioning

confidence: 99%

Screening Cowpea (Vigna unguiculata (L.) Walp.) Genotypes for Enhanced N2 Fixation and Water Use Efficiency under Field Conditions in Ghana

Yahaya¹,

Denwar²,

Mohammed³

et al. 2019

AJPS

View full text Add to dashboard Cite

show abstract

“…Hungria et al [19] also reported that inoculation of soybean significantly increased the nodule number over the control. Attempts realized in Mozambique were also found that some indigenous rhizobia were effective than five reference strains used in the country to improve soybean production [20]. Unlike Doko and Piramama varieties, local rhizobia were unable to increase the number of nodules on Canarana compared IRAT FA3 excepted RSC119, which induced nodules production like the latter.…”

Section: Impact Of Indigenousrhizobia On Nodule Number and Nodule Drymentioning

confidence: 99%

Response of Indigenous Rhizobia to the Inoculation of Soybean [Glycine max (L.) Merrill] Varieties Cultivated under Controlled Conditions in Côte d’Ivoire

Kouadio¹,

Fondio²,

Konaté³

et al. 2020

AiM

View full text Add to dashboard Cite

Soybean [Glycine max (L.) Merrill] is an important crop known to improve population nutritional status and increase soil fertility and its productivity through biological nitrogen fixation. In Côte d'Ivoire, the introduced Bradyrhizobium japonicum used as inoculum had slight compatibility to several soybean varieties compromising their vulgarization. Therefore, the present study was conducted to examine the infectiveness and the effectiveness of indigenous rhizobial isolates on three soybean varieties (Canarana, Doko and Piramana) cultivated in Côte d'Ivoire. The experiment was conducted with potted plant filled with sterilized sand and was statistically laid in Completely Randomized Design (CRD) with sixteen (16) natives rhizobia, one (01) reference strain and uninoculated control (with or without nitrogen) with three replications. The results showed that inoculation significantly improved nodule number, nodule dry weight, plant height and total dry matter of soybean over the negative control treatment. Among the inoculated treatments, five indigenous rhizobia RSC119, RSC324, RSC502, RSC504 and RSC508 significantly (P < 0.05) increased the nodulation and plant growth parameters than B. japonicum (IRAT FA3) with Doko and Piramana. RSC502 produced highest nodule number (64) on Piramama, nodule dry weight was most promoted with RSC504 (321 mg•plant −1 ) compared to IRAT FA3 strain (95 mg•plant −1 ) on Doko. The higher effectiveness was recorded with RSC119, RSC504 and RSC502 with 206.73%; 201.79% and 200.45% respectively compared to TN How to cite this paper: Amani, K., Fondio, L., (100%). The correlation analysis indicated significant association of nodule number and total biomass indicating the importance of symbiotic nitrogen fixation. Based on their infectiveness and their effectiveness, the isolates RSC119, RSC324, RSC502, RSC504 and RSC508 could be used as elite local rhizobia and tested in field conditions to establish their potential contribution on soybean productivity.

show abstract

“…In order for a rhizobial strain to serve as an effective agricultural inoculant, it must be able to (i) fix large amounts of nitrogen and (ii) effectively outcompete the indigenous rhizobial population for nodule occupancy. Unfortunately, the native rhizobial community that is often the most competitive for nodule occupancy tends to exhibit low N 2 fixation rates (for example see A.A. Cardoso et al 2017;Chibeba et al 2017). Indeed, for decades the failure of rhizobia to improve crop yields has often been attributed to the poor competitive abilities of the inoculant and not to poor N 2 fixation abilities (Triplett and Sadowsky 1992;Streeter 1994;Ndungu et al 2018).…”

Section: Synthetic Biology Approaches To Engineering the Symbiosismentioning

confidence: 99%

Multidisciplinary approaches for studying rhizobium–legume symbioses

et al. 2019

View full text Add to dashboard Cite

The rhizobium-legume symbiosis is a major source of fixed nitrogen (ammonia) in the biosphere. The potential for this process to increase agricultural yield while reducing the reliance on nitrogen-based fertilizers has generated interest in understanding and manipulating this process. For decades, rhizobium research has benefited from the use of leading techniques from a very broad set of fields, including population genetics, molecular genetics, genomics, and systems biology. In this review, we summarize many of the research strategies that have been employed in the study of rhizobia and the unique knowledge gained from these diverse tools, with a focus on genome- and systems-level approaches. We then describe ongoing synthetic biology approaches aimed at improving existing symbioses or engineering completely new symbiotic interactions. The review concludes with our perspective of the future directions and challenges of the field, with an emphasis on how the application of a multidisciplinary approach and the development of new methods will be necessary to ensure successful biotechnological manipulation of the symbiosis.

show abstract

Isolation, characterization and selection of indigenous Bradyrhizobium strains with outstanding symbiotic performance to increase soybean yields in Mozambique

Cited by 76 publications

References 66 publications

Screening Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes for Enhanced N<sub>2</sub> Fixation and Water Use Efficiency under Field Conditions in Ghana

Screening Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes for Enhanced N<sub>2</sub> Fixation and Water Use Efficiency under Field Conditions in Ghana

Response of Indigenous Rhizobia to the Inoculation of Soybean [<i>Glycine max</i> (L.) Merrill] Varieties Cultivated under Controlled Conditions in Côte d’Ivoire

Multidisciplinary approaches for studying rhizobium–legume symbioses

Contact Info

Product

Resources

About

Isolation, characterization and selection of indigenous Bradyrhizobium strains with outstanding symbiotic performance to increase soybean yields in Mozambique

Cited by 76 publications

References 66 publications

Screening Cowpea (&lt;i&gt;Vigna unguiculata&lt;/i&gt; (L.) Walp.) Genotypes for Enhanced N&lt;sub&gt;2&lt;/sub&gt; Fixation and Water Use Efficiency under Field Conditions in Ghana

Screening Cowpea (&lt;i&gt;Vigna unguiculata&lt;/i&gt; (L.) Walp.) Genotypes for Enhanced N&lt;sub&gt;2&lt;/sub&gt; Fixation and Water Use Efficiency under Field Conditions in Ghana

Response of Indigenous Rhizobia to the Inoculation of Soybean [&lt;i&gt;Glycine max&lt;/i&gt; (L.) Merrill] Varieties Cultivated under Controlled Conditions in C&#244;te d’Ivoire

Multidisciplinary approaches for studying rhizobium–legume symbioses

Contact Info

Product

Resources

About

Screening Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes for Enhanced N<sub>2</sub> Fixation and Water Use Efficiency under Field Conditions in Ghana

Screening Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes for Enhanced N<sub>2</sub> Fixation and Water Use Efficiency under Field Conditions in Ghana

Response of Indigenous Rhizobia to the Inoculation of Soybean [<i>Glycine max</i> (L.) Merrill] Varieties Cultivated under Controlled Conditions in Côte d’Ivoire