Isolation, characterization and S2−-oxidation metabolic pathway of a sulfur-oxidizing strain from a black-odor river in Beijing

Abstract

A single strain capable of efficient S2−-oxidizing was isolated from a black-odor river in Beijing. The single strain was identified as Stenotrophomonas through the physiology and biochemical characteristics as well as the 16S rRNA sequencing experiment. This strain was named as Stenotrophomonas sp.sp3 (strain sp3). The experimental results showed that for the strain sp3 growth and S2− oxidization, the optimal conditions were as follows: 25 °C of temperature, initial pH 7, 2.5 g/L of initial glucose concentration and 1.00 g/L of initial cell concentration. It was found that there were 31 kinds of sulfur oxidation related genes in the strain sp3 through the whole genomic analysis. The results of the transcriptome analysis suggested that the main metabolic pathway of S2− to SO42− was the paracoccus sulfur oxidation process. The bioconversion processes of S2− to S0, S2− to SO32−, S2O32− to S0 and SO32−, and SO32− to SO42− were controlled by hdrA, cysIJ, tst and sox gene, respectively.

Bio-Augmentation of S2− Oxidation for a Heavily Polluted River by a Mixed Culture Microbial Consortium

“…In this study, three strains with better sulfur oxidation effects were isolated from the Dongsha River, a heavily polluted river located in Beijing, China. These strains, including Citrobacter sp.sp1, Ochrobactrum sp.sp2, and Stenotrophomonas sp.sp3, were used to remove S 2− with mixed culture in our previous work [4,26]. Although the consortium in previous work showed the potential to use S 2− as electron donors, the mechanism involved in the S 2− oxidation process was still unclear.…”