Isolation, characterization and evolution of NBS-LRR encoding disease-resistance gene analogs in eggplant against bacterial wilt

Reddy, Anand C.; Venkat, Sudarshini K.; Singh, T. H.; Aswath, C.; Reddy, K. Madhavi; Reddy, D. C. Lakshmana

doi:10.1007/s10658-015-0693-9

Cited by 13 publications

(16 citation statements)

References 40 publications

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“…These NBS-LRR proteins are mainly of two types, TIR and non-TIR types both involved in pathogen recognition but are distinctive in sequence and signalling pathways (Michale et al 2006). In our study, the cloned sequences belonged to both TIR and non-TIR-NBS-LRR type RGAs, which is similar to results reported in eggplant (Reddy et al 2015), cucumber (Wan et al 2010) and peppers (Wan et al 2012) using the same degenerate primers, indicating the success of homology based PCR cloning of NBS-LRR class disease resistance gene analogs in Capsicum. Out of 28 sequences, CaTRGA1-13 sequences were TIR type having highly conserved aspartic acid (D) at the end of the kinase-2 domain and CaTRGA14-28 sequences were classified as non-TIR type as indicated by the final residue tryptophan (W) at the end of kinase-2 motif.…”

Section: Discussionsupporting

confidence: 86%

“…But in eggplant only non-TIR RGAs were isolated by Zhuang et al (2012). Thus it indicates that the amount and ratio of TIR and non-TIR types varies across the plant species (An and Yang 2014;Reddy et al 2015).…”

Section: Discussionmentioning

confidence: 94%

“…Through transposon tagging using Mu transposon, Hm1 gene, a plant R-gene have been cloned for the first time in maize (Johal and Briggs 1992). Later several R-genes such as N, M, RPF, Gpa2 and others have been cloned in different crops like eggplant (Reddy et al 2015), grapevine (Donald et al 2002), citrus (Deng et al 2000) and other species (Sharma et al 2009). The comparison of the sequences of these R-genes has shown that majority possess conserved amino acid motifs consisting of a NBS domain and a hydrophobic domain (HD) with a consensus amino acid sequence, Gly-Leu-Pro-Leu (GLPL), downstream to NBS (Reddy et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Later several R-genes such as N, M, RPF, Gpa2 and others have been cloned in different crops like eggplant (Reddy et al 2015), grapevine (Donald et al 2002), citrus (Deng et al 2000) and other species (Sharma et al 2009). The comparison of the sequences of these R-genes has shown that majority possess conserved amino acid motifs consisting of a NBS domain and a hydrophobic domain (HD) with a consensus amino acid sequence, Gly-Leu-Pro-Leu (GLPL), downstream to NBS (Reddy et al 2015). Molecular markers facilitate and accelerate the disease resistance breeding programme by reducing the frequency of phenotyping in the process of selection across the generations.…”

Section: Introductionmentioning

confidence: 99%

“…Leister et al (1996) developed a PCR based method to easily isolate R-gene analogues (RGAs), from a wide variety of plant species, in which they used degenerate primers that amplify between the kinase 1a motif of the NB-ARC domain and the GLPL motif. RGAs have been successfully isolated using the PCR based approach from a wide range of plant species (Sharma et al 2009), Cucumis (Wan et al 2010), hot pepper (Wan et al 2012) and wild eggplant (Zhuang et al 2012;Reddy et al 2015). Cloning of resistant genes and development of molecular markers will be highly useful in marker assisted breeding programs.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)

Ponnam

Reddy

et al. 2017

3 Biotech

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Viruses are serious threat to chilli crop production worldwide. Resistance screening against several viruses resulted in identifying a multiple virus resistant genotype 'IHR 2451'. Degenerate primers based on the conserved regions between P-Loop and GLPL of Resistance genes (R-genes) were used to amplify nucleotide binding sites (NBS)-encoding regions from genotype 'IHR 2451'. Alignment of deduced amino acid sequences and phylogenetic analyses of isolated sequences distinguished into two groups representing toll interleukin-1 receptor (TIR) and non-TIR, and different families within the group confirming the hypotheses that dicots have both the types of NBS-LRR genes. The alignment of deduced amino acid sequences revealed conservation of subdomains P-loop, RNBS-A, kinase2, RNBS-B, and GLPL. The distinctive five RGAs showing specific conserved motifs were subjected to BLASTp and indicated high homology at deduced amino acid level with R genes identified such as Pvr9 gene for potyvirus resistance, putative late blight resistance protein homolog R1B-23 and other disease resistance genes suggesting high correlation with resistance to different pathogens. These pepper RGAs could be regarded as candidate sequences of resistant genes for marker development.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)

Ponnam

Reddy

et al. 2017

3 Biotech

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Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

et al. 2021

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Tibetan hulless barley (Hordeum vulgare L. variety nudum Hook. f.) is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Here, we compared the miRNA profiles before and after BLS in a BLS-sensitive ('Z1141') and tolerant ('Kunlun14') Tibetan hulless barley for the first time. A total of 36 conserved and 56 novel miRNAs were identified, of which 24 differentially expressed miRNAs were found in Z1141 and Kunlun14, and an additional 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among these genes, 131 were differentially expressed in normal and infected leaves of Kunlun14 and Z1141.Gene ontology (GO), pathway, and Blast analyses indicated 10 candidate target genes including seven functional genes and three transcription factors were involved in barley disease resistance. These functional genes were two members of the CYP450 genes, a RGA gene, a LIN gene, a SAM gene, a PSD gene, and a NDB gene; the three transcription factor genes were all from the WRKY family. These 10 candidate target genes were regulated by eight miRNAs (hvu-miR168-3p, hvu-miR171-5p, hvu-miR159b, hvu-miR156a, hvu-novel-91, hvu-novel-46, hvu-novel-52, and hvu-novel-11). According to quantitative real-time polymerase chain reaction (PCR) results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Thus, these miRNAs and their target genes can be used to improve BLS resistance in Tibetan hulless barley though breeding programs or genetic engineering.

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Plant-Microbe Interactions: A Molecular Approach

Babar

Khan

Zargaham

et al. 2016

Plant, Soil and Microbes

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Plants thrive in a complex environment comprising of various biotic and abiotic agents. Like all biological systems, these agents tend to interact with the plant body. Microorganisms form a major portion of the ecosystem and have been found to inoculate or infect members of all the kingdoms. Plants and microbes have developed molecular mechanisms to interact with one another and attain the maximum benefi t from the interactions. This mutualistic relationship provides benefi t not only to the microbes but also to the plants. Based upon this complex molecular interplay, a number of mechanisms have been studied and are currently being employed for the agricultural, environmental, and health benefi ts. The principles of biofertilization and bioremediation utilize the plant-microbe interactions for the survival of the two players along with contributing to the food chain and the ecosystem. Similarly, the secondary metabolites obtained from these organisms contribute to human medical and agricultural welfare. These processes are regulated by a variety of biological, physical, chemical, and environmental factors, the study of which can be helpful in exploiting better outcomes from the interaction. The advent of modern techniques has helped in deciphering the role of various molecular players of the plant-microbe interactions. Moreover, they can be employed for regulating the plant-microbe interaction for improved effi ciency. The current chapter discusses the molecular mechanisms involved in the plant-microbe interactions exhibited in biofertilization, bioremediation, biocontrol, and induced systemic resistance. Afterwards, the factors affecting the molecular machinery involved in these pathways have been discussed. Toward the end, a brief introduction of the genetic 2 manipulative techniques and their applications in the plant-microbe interactions has been presented.

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Isolation, characterization and evolution of NBS-LRR encoding disease-resistance gene analogs in eggplant against bacterial wilt

Cited by 13 publications

References 40 publications

Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)

Isolation, characterization and genetic diversity of NBS-LRR class disease-resistant gene analogs in multiple virus resistant line of chilli (Capsicum annuum L.)

Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

Plant-Microbe Interactions: A Molecular Approach

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