Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Abstract

Developing wisdom teeth are easy-accessible source of stem cells during the adulthood which could be obtained by routine orthodontic treatments. Human pulp-derived stem cells (hDPSCs) possess high proliferation potential with multi-lineage differentiation capacity compare to the ordinary source of adult stem cells [1][2][3][4][5][6][7][8]

How has dental pulp stem cells isolation been conducted? A scoping review

“…The DPSCs isolation process has evolved overtime and currently it can be done using two main techniques: explant 18,19,20 and enzymatic; 19,20 the Table 4. Distribution according to the supplementation used in DPSCs culture medium preparation (some studies used more than one culture medium).…”

The Effect of two Different Culture Media on the Proliferation of Dental Pulp Stem Cells: Bovine Fetal Serum and Platelet Lysate

“…In particular there is a higher percentage of cells with CD45+ characteristics and a lower percentage of CD90, CD 105positive cells (Table 2). These results suggest that experimental population of cells obtained in vitro isa mixed population and does not represent the characteristics of a pure mesenchymal stem cell population that was expected to be seen (Karamzadeh et al, 2012).…”

Dental Stem Cells Characterization and Bone Regenerative Potential in Oral Medicine

“…Other methods like Magnetic activated cell sorting (MACS) and Fluorescence activated cell sorting (FACS) are also available [17,[24][25][26][27].…”

“…The next step is disinfection followed by another washing with PBSA. Pulp tissue is isolated from the pulp chamber and placed into a sterile petri dish, washed at least three times with PBSA [17,25,26].…”

“…Suspensions are then seeded in culture dishes containing proliferation medium and incubated at 37°C in 5% CO 2 . Culture medium can be substituted every three days until cell confluence is achieved [17,[24][25][26][27].…”